Ultrastructural organization of Rhizophlyctis harderi zoospores and redefinition of the type 1 microbody – lipid globule complex

1992 ◽  
Vol 70 (4) ◽  
pp. 750-761 ◽  
Author(s):  
Martha J. Powell ◽  
Sonali Roychoudhury
Keyword(s):  
Fine Structure ◽  
Three Dimensional ◽  
Ultrastructural Organization ◽  
Serial Sections ◽  
Dense Cores ◽  
Lipid Globule ◽  
Computer Aided ◽  
Glycogen Particles ◽  
Peripheral Cytoplasm

Because ultrastructural features of zoospores are considered primary characters in Chytridiomycete systematics, computer-aided three-dimensional reconstructions of serial sections were used to analyze zoospore fine structure of Rhizophlyctis harderi, a questionable member of the genus Rhizophlyctis. A secondary centriole was parallel to the kinetosome, but the nucleus was not structurally or spatially associated with the kinetosomes. Mitochondria and cisternae associated with vesicles bounded a ribosomal aggregation in which the nucleus was partially embedded. The peripheral cytoplasm between the plasma membrane and ribosomal aggregation contained α-glycogen particles, vacuoles with osmiophilic globules, vesicles with clear matrices, and vesicles with electron-dense cores. A new, compound form of microbody – lipid globule complex (MLC) was identified. This type of MLC incorporated posteriorly located lipid globules associated with rumposomes and anteriorly located lipid globules associated with simple cisternae, microbodies, and highly branched mitochondria. Based on these and other recent observations, the concept of the type 1 MLC was redefined. Sources for variation in ultrastructural features of zoospores are discussed. Zoospore ultrastructure of R. harderi is different from that described for other chytrid zoospores. Key words: Chytridiomycetes, microbody – lipid globule complex, Rhizophlyctis, taxonomy, ultrastructure, zoospore.

scholarly journals Three-dimensional ultrastructure of a unicellular cyanobacterium.

1983 ◽  
Vol 97 (3) ◽  
pp. 713-722 ◽  
Author(s):  
S A Nierzwicki-Bauer ◽  
D L Balkwill ◽  
S E Stevens
Keyword(s):  
Inclusion Bodies ◽  
Cytoplasmic Membrane ◽  
Three Dimensional ◽  
Thylakoid Membranes ◽  
Serial Sections ◽  
Unicellular Cyanobacterium ◽  
High Voltage Electron Microscopy ◽  
Voltage Electron ◽  
Inner Surface ◽  
Computer Aided

The first complete three-dimensional ultrastructural reconstruction of a cyanobacterium was accomplished with high-voltage electron microscopy and computer-aided assembly of serial sections. The precise arrangement of subcellular features within the cell body was very consistent from one cell to another. Specialized inclusion bodies always occupied specific intracellular locations. The photosynthetic thylakoid membranes entirely surrounded the central portion of the cytoplasm, thereby compartmentalizing it from the rest of the cell. The thylakoid membranes formed an interconnecting network of concentric shells, merging only at the inner surface of the cytoplasmic membrane. The thylakoids were in contact with the cytoplasmic membrane at several locations, apparently to maintain the overall configuration of the thylakoid system. These results clarified several unresolved issues regarding structure-function relationships in cyanobacteria.

Three dimensional structure of the leishmania amastigote as revealed by computer-aided reconstruction from serial sections

Parasitology ◽  
1986 ◽  
Vol 92 (1) ◽  
pp. 13-23 ◽  
Author(s):  
G. H. Coombs ◽  
L. Tetley ◽  
V. A. Moss ◽  
K. Vickerman
Keyword(s):  
Cell Volume ◽  
Three Dimensional ◽  
Spatial Arrangement ◽  
Dimensional Structure ◽  
Serial Sections ◽  
Leishmania Mexicana ◽  
Cell Organelles ◽  
3 Dimensional ◽  
Computer Aided ◽  
Total Cell Volume

SUMMARYComputer-aided reconstruction from serial sections has been used to analyse the 3-dimensional structure of entire amastigotes of Leishmania mexicana mexicana and to determine the number, arrangement and volume of each organelle. In two reconstructions, the lysosome-like ‘megasomes’ were the most numerous organelle, there being 34 in one amastigote, and they comprised as much as 15% of the total cell volume. In contrast, as few as 9 glycosomes were present, accounting for less than 1% of the cell volume. The unitary nature of the mitochondrion was confirmed and its complex basket-like structure was revealed. The spatial arrangement of the cell organelles is here displayed in stereo-pairs.

Three-dimensional morphometry of biological fine structure using HVEM stereo images

1991 ◽  
Vol 49 ◽  
pp. 432-433
Author(s):  
Kiyoshi Hama
Keyword(s):  
Fine Structure ◽  
Tilt Angle ◽  
Three Dimensional ◽  
Serial Sections ◽  
Analysis Method ◽  
Stereo Images ◽  
Advantages And Disadvantages ◽  
Paired Electron ◽  
Fine Structures ◽  
The Given

There are several approaches of extracting three dimensional (3D) morphometrical information from biological fine structures, computer 3D reconstruction using thin serial sections, stereologica1 analysis of random thin section images, reconstruction from the results obtained by Fourier analysis of continuously tilted images, and so on. We have developed a computer analysis method of HVEM stereo images of thick biological specimens. Each method has advantages and disadvantages and one can choose one or other method according to the nature of samples and/or the nature of the required information.In our method, stereo paired electron micrographs are processed to produce skeletonized images, they are automatically traced, and the z coordinates of the given specimen points are calculated from the parallaxes between the two tilted images and the tilt angle.

Computer-aided three-dimensional reconstruction of nematode embryos from EM serial sections

1986 ◽  
Vol 166 (1) ◽  
pp. 247-252 ◽  
Author(s):  
Einhard Schierenberg ◽  
Tom Cole ◽  
Christopher Carlson ◽  
Wayne Sidio
Keyword(s):  
Three Dimensional ◽  
Serial Sections ◽  
Three Dimensional Reconstruction ◽  
Computer Aided ◽  
Dimensional Reconstruction

The functional organization and fine structure of the tail musculature of the cercariae of Cryptocotyle lingua and Himasthla secunda

Parasitology ◽  
1973 ◽  
Vol 66 (3) ◽  
pp. 487-497 ◽  
Author(s):  
H. D. Chapman
Keyword(s):  
Fine Structure ◽  
Functional Organization ◽  
Three Dimensional ◽  
Muscle Cells ◽  
Serial Sections ◽  
Three Dimensional Reconstruction ◽  
Transmission Electron ◽  
Dimensional Reconstruction ◽  
Electron Microscopes

The functional organization of the tail musculature of two species of cercariae has been studied by examination of serial sections in the light and transmission electron microscopes. A three-dimensional reconstruction of the tail has been established. The fine structure of the muscle cells has been investigated and the complexity of organization is related to the requirement for a fast contracting system in the cercarial tail.

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