Environmental persistence of Tulane virus — a surrogate for human norovirus

2016 ◽  
Vol 62 (5) ◽  
pp. 449-454 ◽  
Author(s):  
Sabastine Eugene Arthur ◽  
Kristen Elizabeth Gibson
Keyword(s):  
Culture System ◽  
Viral Gastroenteritis ◽  
Cell Culture System ◽  
Environmental Waters ◽  
Environmental Persistence ◽  
Human Norovirus ◽  
Tulane Virus ◽  
A Cell ◽  
D Values ◽  
Acute Viral Gastroenteritis

Human noroviruses (HuNoVs) are the leading cause of acute viral gastroenteritis worldwide. The persistence of HuNoV in the environment contributes significantly to its transmission to humans. Surrogate viruses are used to study HuNoV owing to the lack of a cell culture system for this virus. Here, the persistence of Tulane virus (TV) — a novel HuNoV surrogate — in surface water (SW) and groundwater (GW) as well as on acrylic-based solid (ABS) and stainless steel (SS) surfaces was investigated. After 28 days, TV remained stable in SW (<1 log10 reduction) but was reduced by ≥3.5 to 4 log10 in GW by day 21. TV had a higher rate of reduction on SS compared with ABS, with corresponding D values of 18.5 ± 0.34 and 13.1 ± 0.36 days, respectively. This is the first study to demonstrate the persistence of TV in environmental waters and on fomite surfaces.

scholarly journals Dasabuvir inhibits human norovirus infection in human intestinal enteroids

2021 ◽  
Author(s):  
Tsuyoshi Hayashi ◽  
Kosuke Murakami ◽  
Junki Hirano ◽  
Yoshiki Fujii ◽  
Yoko Yamaoka ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Severe Acute Respiratory Syndrome ◽  
Culture System ◽  
Age Groups ◽  
Viral Gastroenteritis ◽  
Compound Library ◽  
Human Norovirus ◽  
Antiviral Compound ◽  
Acute Viral Gastroenteritis

Human noroviruses (HuNoVs) are acute viral gastroenteritis pathogens that affect all age groups, yet no approved vaccines and drugs to treat HuNoV infection are available. In this study, with a human intestinal enteroid (HIE) culture system where HuNoVs are able to replicate reproducibly, we screened an antiviral compound library to identify compound(s) showing anti-HuNoV activity. Dasabuvir, which has been developed as an anti-hepatitis C virus agent, was found to inhibit HuNoV infection in HIEs at micromolar concentrations. Dasabuvir also inhibited severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and human A rotavirus (RVA) infection in HIEs. To our knowledge, this is the first study to screen an antiviral compound library for HuNoV using HIEs and we successfully identified dasabuvir as a novel anti-HuNoV inhibitor that warrants further investigation.

A Cell Culture System for Study of the Development of Toxoplasma gondii Bradyzoites

1995 ◽  
Vol 42 (2) ◽  
pp. 150-157 ◽  
Author(s):  
LOUIS M. WEISS ◽  
DENISE LAPLACE ◽  
PETER M. TAKVORIAN ◽  
HERBERT B. TANOWITZ ◽  
ANN CALI ◽  
...  
Keyword(s):  
Cell Culture ◽  
Toxoplasma Gondii ◽  
Culture System ◽  
Cell Culture System ◽  
A Cell

scholarly journals ISOLATION OF VIRUS CAUSING HEMORRHAGIC FEVER WITH RENAL SYNDROME (HFRS) THROUGH A CELL CULTURE SYSTEM

1983 ◽  
Vol 36 (1) ◽  
pp. 17-25 ◽  
Author(s):  
TAKASHI KITAMURA ◽  
TOSHIHIKO KOMATSU ◽  
KAZUYOSHI SUGIYAMA ◽  
CHIHARU MORITA ◽  
KIYOSHI IMAIZUMI ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Hemorrhagic Fever ◽  
Cell Culture System ◽  
A Cell

The Development and Characterization of a Cell Culture System from Indian Mud Crabs Scylla serrata

2019 ◽  
Vol 31 (3) ◽  
pp. 244-258
Author(s):  
Selvaraj Sivakumar ◽  
T. Raja Swaminathan ◽  
Raj Kumar ◽  
Natarajan Kalaimani
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Scylla Serrata ◽  
Cell Culture System ◽  
A Cell ◽  
Mud Crabs
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