Characterization of Bacillus thuringiensis subsp. kurstaki strain S93 effective against the fall armyworm Spodoptera frugiperda)

1999 ◽  
Vol 45 (6) ◽  
pp. 464-471 ◽  
Author(s):  
Joseilde O. Silva-Werneck ◽  
Marlene T. De-Souza ◽  
José M.C. de S. Dias ◽  
Bergmann M. Ribeiro
Keyword(s):  
Bacillus Thuringiensis ◽  
Spodoptera Frugiperda ◽  
Fall Armyworm

scholarly journals Molecular Characterization of Bacillus thuringiensis cyt Genes and Their Effect Against Fall Armyworm, Spodoptera frugiperda

2014 ◽  
Vol 6 (7) ◽  
Author(s):  
Maria L. M. Costa ◽  
Ubiraci G. P. Lana ◽  
Emerson C. Barros ◽  
Luciano V. Paiva ◽  
Fernando H. Valicente
Keyword(s):  
Bacillus Thuringiensis ◽  
Molecular Characterization ◽  
Spodoptera Frugiperda ◽  
Fall Armyworm

Virulence and Genetic Characterization of Six Baculovirus Strains Isolated From Different Populations of Spodoptera Frugiperda (Lepidoptera: Noctuidae)

2021 ◽  
Author(s):  
Ingrid Zanella-Saenz ◽  
Elisabeth A. Herniou ◽  
Jorge E. Ibarra ◽  
Ma.Cristina Del Rincón-Castro ◽  
Ilse Alejandra Huerta-Arredondo
Keyword(s):  
Biological Control ◽  
Cell Cultures ◽  
Spodoptera Frugiperda ◽  
Fall Armyworm ◽  
The United States ◽  
Infection Mechanisms ◽  
Different Populations

Abstract Fall armyworm (FAW), Spodoptera frugiperda (Smith, 1797), is a polyphagous, voracious, and economically important agricultural pest. Biological control of FAW is a strategy that must be further explored. This study evaluated six baculovirus strains isolated from infected FAW larvae from Mexico, Argentina, Honduras, and the United States. Five alphabaculoviruses (SfNPV-An2, SfNPV-Arg, SfNPV-Fx, SfNPV-Ho and SfNPV-Sin) and one betabaculovirus (SfGV-RV), were tested against FAW larvae, showing a wide diversity of virulence levels among strains when their estimated LC50s were compared, being SfNPVArg, SfNPV-Ho and SfNPV-Fx more virulent than SfNPV-An 2 , SfNPV-Sin and SfGV-RV. To determine any virulence difference in vitro studies of these isolates, Sf9 cell cultures were used. Interestingly, only ODVs from four of the test SfNPV strains showed infectivity on Sf9 cell cultures, and some differences in virulence were observed. Genomic restriction analyses and partial sequences of lef-8, lef-9 , and polh/granulin genes showed little variability among alphabaculoviruses, both, among them and with previously reported sequences. However, sequences from SfGV-RV were closer to previously reported sequences from the SfGVVG008 strain than the SfGV-Arg and SfGV-VG014 strains. The great difference in the in vivo virulence was not correlated with great similarity among the isolates. The characterization of these six baculoviruses isolates offers the basis for exploring their potential as biological control agents against S. frugiperda, as well the initial studies on their specific infection mechanisms, evolution, and ecology.

Molecular characterization and distribution of Bacillus thuringiensis cry1 genes from Brazilian strains effective against the fall armyworm, Spodoptera frugiperda

2010 ◽  
Vol 53 (3) ◽  
pp. 360-366 ◽  
Author(s):  
Fernando Hercos Valicente ◽  
Edgard Augusto de Toledo Picoli ◽  
Maria José Vilaça de Vasconcelos ◽  
Newton Portilho Carneiro ◽  
Andréia Almeida Carneiro ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Molecular Characterization ◽  
Spodoptera Frugiperda ◽  
Fall Armyworm ◽  
Cry1 Genes

scholarly journals Insecticidal activity of culture supernatants from Bacillus thuringiensis Berliner strains against Spodoptera frugiperda Smith (Lepidoptera: Noctuidae) larvae

1999 ◽  
Vol 28 (4) ◽  
pp. 675-685 ◽  
Author(s):  
Marliton R. Barreto ◽  
Leandro L. Loguercio ◽  
Fernando H. Valicente ◽  
Edilson Paiva
Keyword(s):  
Bacillus Thuringiensis ◽  
Protein Secretion ◽  
Spodoptera Frugiperda ◽  
Wide Spectrum ◽  
Insect Pest ◽  
Polyacrylamide Gel Electrophoresis ◽  
Fall Armyworm ◽  
Tropical Maize ◽  
Potential Applicability ◽  
Culture Supernatants

Novel vegetative insecticidal proteins (Vips) identified in the supernatant of Bacillus thuringiensis (B.t.) cultures have shown to provide adequate control over a wide spectrum of economically important crop pests. To evaluate the potential applicability of these proteins against fall armyworm (Spodoptera frugiperda Smith) larvae, the most important insect pest for tropical maize, the characteristics and mortality effects of culture supernatants from five B.t. strains were investigated. Striking differences among strains were detected, not only in terms of efficiency in killing the insect, but also regarding to mortality effects of heated and non-heated supernatants, which were used to distinguish the heat-sensitive protein-derived insecticidal fraction from a thermostable one, with a non-protein nature (b-exotoxinas). The qualitative, quantitative and temporal patterns of total protein secretion in the medium (supernatant) were assessed through spectrophotometry and polyacrylamide gel electrophoresis. The strains showed remarkably distinct rates of growth and timing for protein secretion relative to cell density in culture. Moreover, the electrophoretic-banding patterns also varied in a strain-specific manner, both in denaturing and non denaturing conditions. Polypeptides displaying a molecular weight that is very close to the expected for previously identified Vip3A proteins were found for the strains with high supernatant-mortality ratios. The data suggest the feasibility and usefulness of searching for protein-derived (Vip-like) insecticidal fractions in B.t. supernatants as a mean of developing especific and efficient alternatives of biological control to be employed in integrated pest management programs of S. frugiperda in tropical maize.

Selection and characterization of the Bacillus thuringiensis strains toxic to Spodoptera eridania (Cramer), Spodoptera cosmioides (Walker) and Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae)

2009 ◽  
Vol 50 (2) ◽  
pp. 157-163 ◽  
Author(s):  
Karen Bianchi dos Santos ◽  
Pedro Neves ◽  
Ana Maria Meneguim ◽  
Rachel Bianchi dos Santos ◽  
Walter Jorge dos Santos ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Spodoptera Frugiperda ◽  
Spodoptera Eridania ◽  
Lepidoptera Noctuidae

scholarly journals Characterization of field‐evolved resistance to Bacillus thuringiensis ‐derived Cry1F δ‐endotoxin in Spodoptera frugiperda populations from Argentina

2017 ◽  
Vol 74 (3) ◽  
pp. 746-754 ◽  
Author(s):  
Desmi I Chandrasena ◽  
Ana M Signorini ◽  
Gustavo Abratti ◽  
Nicholas P Storer ◽  
Magdalena L Olaciregui ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Spodoptera Frugiperda

scholarly journals Biorational management of maize fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) using Bacillus thuringiensis (Berliner) enriched with chemical additives

2021 ◽  
Vol 13 (4) ◽  
pp. 1231-1237
Author(s):  
M. Priyanka ◽  
P. Yasodha ◽  
C.Gailce Leo Justin ◽  
J. Ejilane ◽  
Venugopal Rajanbabu
Keyword(s):  
Bacillus Thuringiensis ◽  
Boric Acid ◽  
Sodium Nitrate ◽  
Spodoptera Frugiperda ◽  
Seedling Emergence ◽  
Fall Armyworm ◽  
Invasive Pest ◽  
Chemical Additives ◽  
Stage Of Development ◽  
Lepidoptera Noctuidae

An invasive pest, fall armyworm, Spodoptera frugiperda (J.E.Smith) (Lepidoptera: Noctuidae) attacks maize at every stage of development, from seedling emergence up to cob formation. Early instar larvae were seen mostly on leaves of maize with characteristics pin or shot hole symptoms. Later instar larvae were confined to deep whorls, leaving typically ragged like appearance and fed on the reproductive stage of the crop especially tassels and developing cobs resulting in quality and quantity loss of maize produce. The effect of commercially available Bacillus thuringiensis subsp. kurstaki product, Dipel® against the second instar larvae of Fall Armyworm (FAW )was not promising under laboratory conditions. Hence, an effort was made to add an adjuvant along with B. thuringiensis to increase the virulence of commercially available B. thuringiensis.The Laboratory bioassays with B. thuringiensis and seven chemical additives ( T1- Bt + Boric acid, T2- Bt + Zinc oxide, T3- Bt + Sodium nitrate, T4- Bt + Peptone, T5- Bt + Urea, T6- Bt + EDTA, T7- Bt + Citric acid & T8-  Bt alone T9- Control) were tested against second instar larvae of Spodoptera frugiperda larvae. The results showed that B. thuringiensis plus sodium nitrate (T3) promoted maximum mortality 82.2 per cent with a minimum LC50 value of 54.620 mg/l. Sodium nitrate boosted B. thuringiensis activity at a concentration of 0.05 per cent by 2.128-fold than B. thuringiensis alone. Overall, sodium nitrate improved the efficacy of B. thuringiensis spray at the maximum level followed by boric acid, urea, EDTA and peptone.

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