Interactions between a commercial preparation of Bacillus thuringiensis and β-exotoxin in the fall armyworm, Spodoptera frugiperda

1987 ◽  
Vol 50 (3) ◽  
pp. 201-206 ◽  
Author(s):  
Mark D. Mueller ◽  
James D. Harper
Keyword(s):  
Bacillus Thuringiensis ◽  
Spodoptera Frugiperda ◽  
Fall Armyworm ◽  
Commercial Preparation

Molecular characterization and distribution of Bacillus thuringiensis cry1 genes from Brazilian strains effective against the fall armyworm, Spodoptera frugiperda

2010 ◽  
Vol 53 (3) ◽  
pp. 360-366 ◽  
Author(s):  
Fernando Hercos Valicente ◽  
Edgard Augusto de Toledo Picoli ◽  
Maria José Vilaça de Vasconcelos ◽  
Newton Portilho Carneiro ◽  
Andréia Almeida Carneiro ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Molecular Characterization ◽  
Spodoptera Frugiperda ◽  
Fall Armyworm ◽  
Cry1 Genes

scholarly journals Insecticidal activity of culture supernatants from Bacillus thuringiensis Berliner strains against Spodoptera frugiperda Smith (Lepidoptera: Noctuidae) larvae

1999 ◽  
Vol 28 (4) ◽  
pp. 675-685 ◽  
Author(s):  
Marliton R. Barreto ◽  
Leandro L. Loguercio ◽  
Fernando H. Valicente ◽  
Edilson Paiva
Keyword(s):  
Bacillus Thuringiensis ◽  
Protein Secretion ◽  
Spodoptera Frugiperda ◽  
Wide Spectrum ◽  
Insect Pest ◽  
Polyacrylamide Gel Electrophoresis ◽  
Fall Armyworm ◽  
Tropical Maize ◽  
Potential Applicability ◽  
Culture Supernatants

Novel vegetative insecticidal proteins (Vips) identified in the supernatant of Bacillus thuringiensis (B.t.) cultures have shown to provide adequate control over a wide spectrum of economically important crop pests. To evaluate the potential applicability of these proteins against fall armyworm (Spodoptera frugiperda Smith) larvae, the most important insect pest for tropical maize, the characteristics and mortality effects of culture supernatants from five B.t. strains were investigated. Striking differences among strains were detected, not only in terms of efficiency in killing the insect, but also regarding to mortality effects of heated and non-heated supernatants, which were used to distinguish the heat-sensitive protein-derived insecticidal fraction from a thermostable one, with a non-protein nature (b-exotoxinas). The qualitative, quantitative and temporal patterns of total protein secretion in the medium (supernatant) were assessed through spectrophotometry and polyacrylamide gel electrophoresis. The strains showed remarkably distinct rates of growth and timing for protein secretion relative to cell density in culture. Moreover, the electrophoretic-banding patterns also varied in a strain-specific manner, both in denaturing and non denaturing conditions. Polypeptides displaying a molecular weight that is very close to the expected for previously identified Vip3A proteins were found for the strains with high supernatant-mortality ratios. The data suggest the feasibility and usefulness of searching for protein-derived (Vip-like) insecticidal fractions in B.t. supernatants as a mean of developing especific and efficient alternatives of biological control to be employed in integrated pest management programs of S. frugiperda in tropical maize.

scholarly journals Biorational management of maize fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) using Bacillus thuringiensis (Berliner) enriched with chemical additives

2021 ◽  
Vol 13 (4) ◽  
pp. 1231-1237
Author(s):  
M. Priyanka ◽  
P. Yasodha ◽  
C.Gailce Leo Justin ◽  
J. Ejilane ◽  
Venugopal Rajanbabu
Keyword(s):  
Bacillus Thuringiensis ◽  
Boric Acid ◽  
Sodium Nitrate ◽  
Spodoptera Frugiperda ◽  
Seedling Emergence ◽  
Fall Armyworm ◽  
Invasive Pest ◽  
Chemical Additives ◽  
Stage Of Development ◽  
Lepidoptera Noctuidae

An invasive pest, fall armyworm, Spodoptera frugiperda (J.E.Smith) (Lepidoptera: Noctuidae) attacks maize at every stage of development, from seedling emergence up to cob formation. Early instar larvae were seen mostly on leaves of maize with characteristics pin or shot hole symptoms. Later instar larvae were confined to deep whorls, leaving typically ragged like appearance and fed on the reproductive stage of the crop especially tassels and developing cobs resulting in quality and quantity loss of maize produce. The effect of commercially available Bacillus thuringiensis subsp. kurstaki product, Dipel® against the second instar larvae of Fall Armyworm (FAW )was not promising under laboratory conditions. Hence, an effort was made to add an adjuvant along with B. thuringiensis to increase the virulence of commercially available B. thuringiensis.The Laboratory bioassays with B. thuringiensis and seven chemical additives ( T1- Bt + Boric acid, T2- Bt + Zinc oxide, T3- Bt + Sodium nitrate, T4- Bt + Peptone, T5- Bt + Urea, T6- Bt + EDTA, T7- Bt + Citric acid & T8-  Bt alone T9- Control) were tested against second instar larvae of Spodoptera frugiperda larvae. The results showed that B. thuringiensis plus sodium nitrate (T3) promoted maximum mortality 82.2 per cent with a minimum LC50 value of 54.620 mg/l. Sodium nitrate boosted B. thuringiensis activity at a concentration of 0.05 per cent by 2.128-fold than B. thuringiensis alone. Overall, sodium nitrate improved the efficacy of B. thuringiensis spray at the maximum level followed by boric acid, urea, EDTA and peptone.

scholarly journals Bacillus thuringiensisCry1Da_7 and Cry1B.868 Protein Interactions with Novel Receptors Allow Control of Resistant Fall Armyworms,Spodoptera frugiperda(J.E. Smith)

2019 ◽  
Vol 85 (16) ◽  
Author(s):  
Yanfei Wang ◽  
Jinling Wang ◽  
Xiaoran Fu ◽  
Jeffrey R. Nageotte ◽  
Jennifer Silverman ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Protein Interactions ◽  
Spodoptera Frugiperda ◽  
Insect Cells ◽  
Fall Armyworm ◽  
Insecticidal Protein ◽  
Row Crops ◽  
Content Type ◽  
Resistance Risk ◽  
Important Pest

ABSTRACTTwo new modifiedBacillus thuringiensis(Bt) proteins, Cry1Da_7 and Cry1B.868, with activity against fall armyworms (FAW),Spodoptera frugiperda(J.E. Smith), were evaluated for their potential to bind new insect receptors compared to proteins currently deployed as plant-incorporated protectants (PIPs) in row crops. Results from resistant insect bioassays, disabled insecticidal protein (DIP) bioassays, and cell-based assays using insect cells expressing individual receptors demonstrate that receptor utilizations of the newly modified Cry1Da_7 and Cry1B.868 proteins are distinct from each other and from those of commercially availableBtproteins such as Cry1F, Cry1A.105, Cry2Ab, and Vip3A. Accordingly, these two proteins target different insect proteins in FAW midgut cells and when pyramided together should provide durability in the field against this economically important pest.IMPORTANCEThere is increased concern with the development of resistance to insecticidal proteins currently expressed in crop plants, especially against high-resistance-risk pests such as fall armyworm (FAW),Spodoptera frugiperda, a maize pest that already has developed resistance toBacillus thuringiensis(Bt) proteins such as Cry1F. Lepidopteran-specific proteins that bind new insect receptors will be critical in managing current Cry1F-resistant FAW and delaying future resistance development. Results from resistant insect assays, disabled insecticidal protein (DIP) bioassays, and cell-based assays using insect cells expressing individual receptors demonstrate that target receptors of the Cry1Da_7 and Cry1B.868 proteins are different from each other and from those of commercially availableBtproteins such as Cry1F, Cry1A.105, Cry2Ab, and Vip3A. Therefore, pyramiding these two new proteins in maize will provide durable control of this economically important pest in production agriculture.

scholarly journals Efecto de la aplicación de Bacillus thuringiensis en el control del gusano cogollero (Spodoptera frugiperda J.E. Smith) del híbrido de Maíz (Zea mays) INIAP H-551 en la comuna Río Verde provincia de Santa Elena

2018 ◽  
Vol 5 (1) ◽  
pp. 47-56
Author(s):  
Andres Drouet Candell
Keyword(s):  
Zea Mays ◽  
Bacillus Thuringiensis ◽  
Spodoptera Frugiperda ◽  
Fall Armyworm ◽  
Control Treatment ◽  
Economic Damage ◽  
Harmful Substances ◽  
Santa Elena ◽  
Absolute Control ◽  
Different Doses

El maíz es uno de los principales cultivos que se siembra en el litoral ecuatoriano, atacado fundamentalmente por  Spodoptera frugiperda.  Por lo que esta investigación tiene como objetivo evaluar el efecto de la aplicación de Bacillus thuringiensis (BT) en el control del gusano cogollero (Spodoptera frugiperda) del híbrido de maíz (Zea mays) INIAP H-551 en la comuna Río Verde provincia de Santa Elena. Se realizó una investigación aplicada y experimental, en la que se estudiaron dos variables: el efecto del BT sobre el cogollero y la población del cogollero en el cultivo de maíz. Se establecieron siete tratamientos, con cinco dosis diferentes de BT, las dosis fueron comparadas con dos tratamientos, uno químico y el tratamiento testigo. Se demostró que el testigo absoluto fue el tratamiento más atacado y que las aplicaciones de BT  y de cipermetrina no permitieron que se llegara al umbral de daño económico del cultivo. Se recomienda emplear el BT como una alternativa tecnológica limpia para el control del gusano cogollero, con el fin de disminuir la contaminación de los suelos y las aguas, así como evitar que se introduzcan sustancias nocivas en la cadena alimenticia. ABSTRACT Corn is a major crop that is planted in the Ecuadorian coastline, primarily attacked by Spodoptera frugiperda. So, this research aims to evaluate the effect of the application of Bacillus thuringiensis (BT) in controlling fall armyworm (Spodoptera frugiperda) of hybrid corn (Zea mays) INIAP H-551 in Rio Verde commune province of Santa Elena. An applied and experimental research was conducted in which two variables were studied: the effect on BT armyworm and fall armyworm population in maize. Seven treatments were established with five different doses of BT, doses were compared with two treatments, one chemical and the control treatment. It was demonstrated that the absolute control was the most targeted treatment and applications of BT and cypermethrin did not allow it reached the threshold of economic damage to the crop. It is recommended to use BT technology as a clean alternative for armyworm control in order to reduce contamination of soil and water, as well as prevent harmful substances entering the food chain.

scholarly journals Susceptibility of Anthonomus grandis (Cotton Boll Weevil) and Spodoptera frugiperda (Fall Armyworm) to a Cry1Ia-type Toxin from a Brazilian Bacillus thuringiensis Strain

BMB Reports ◽  
2007 ◽  
Vol 40 (5) ◽  
pp. 773-782 ◽  
Author(s):  
Maria Fatima Grossi-De-Sa ◽  
Mariana Quezado De Magalhaes ◽  
Marilia Santos Silva ◽  
Shirley Margareth.Buffon Silva ◽  
Simoni Campos Dias ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Spodoptera Frugiperda ◽  
Fall Armyworm ◽  
Boll Weevil ◽  
Anthonomus Grandis ◽  
Cotton Boll ◽  
Cotton Boll Weevil ◽  
Thuringiensis Strain

Concentration–Mortality Response of Mexican Populations Fall Armyworm (Lepidoptera: Noctuidae) to Commercial Formulations of Bacillus Thuringiensis

2021 ◽  
Vol 56 (1) ◽  
pp. 70-83
Author(s):  
María del Carmen Lara-Becerra ◽  
Manuel Alejandro Tejeda-Reyes ◽  
Ángel Lagunes-Tejeda ◽  
Gonzalo Silva-Aguayo ◽  
J. Concepción Rodríguez-Maciel
Keyword(s):  
Bacillus Thuringiensis ◽  
Spodoptera Frugiperda ◽  
Zea Mays L ◽  
Fall Armyworm ◽  
Mortality Response ◽  
Important Pest ◽  
Median Lethal Concentrations ◽  
Lepidoptera Noctuidae ◽  
Laboratory Bioassays ◽  
Mexican Populations

Abstract The fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), is the most economically important pest of maize (Zea mays L.) grown in Mexico. In order to identify biologically based management tactics for this pest, we determined the concentration–mortality response of 16 S. frugiperda populations collected in Mexico to two commercial formulations of Bacillus thuringiensis Berliner (Bt) (XenTari® [Bt subsp. aizawai] and DiPel® [Bt subsp. kurstaki]; Valent de México SA De CV, Jalisco, México). Laboratory bioassays established median lethal concentrations (LC50s) for each Bt formulation × population combination. We also determined mean larval weight 7 d after exposure and the number of larvae that reached the third instar of development. The populations were susceptible to both Bt formulations but they were, overall, more susceptible to Bt subsp. aizawai (XenTari) than to Bt subsp. kurstaki (DiPel). These results can serve as a reference to detect changes in S. frugiperda response to these Bt commercial products over time.

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