CULTIVATION OF POLIOMYELITIS VIRUS IN TISSUE CULTURE

1952 ◽  
Vol 30 (3) ◽  
pp. 231-245 ◽  
Author(s):  
Joan C. Thicke ◽  
Darline Duncan ◽  
William Wood ◽  
A. E. Franklin ◽  
A. J. Rhodes
Keyword(s):  
Tissue Culture ◽  
Cell Morphology ◽  
Synthetic Medium ◽  
Embryonic Brain ◽  
Human Embryonic Kidney ◽  
Virus Growth ◽  
Nutritive Medium ◽  
Poliomyelitis Virus ◽  
Cytopathogenic Effect ◽  
And Control

This paper presents observations on the growth of Lansing poliomyelitis virus in fluid cultures of various human embryonic and adult tissues. The evidence suggests that viral multiplication has occurred in cultures of monkey testis, human embryonic kidney, and mixtures of brain and cord. Satisfactory virus growth has been obtained particularly in cultures containing human embryonic brain and cord. Virus is present in tissue culture fluids in which the original inoculum has been diluted 10−33.3 by subcultivation. Preliminary observations suggest that a synthetic medium (Mixture 199) devised by Morgan, Morton, and Parker is superior to Hanks–Simms solution as a nutritive medium in such cultures. The cytopathogenic effect of the virus, as revealed by pH determinations and cell morphology, has been noted, although a characteristic pH differential between virus infected and control flasks was not commonly observed. Attempts to grow the virus on a larger scale in Kolle flasks are described.

CULTIVATION OF POLIOMYELITIS VIRUS IN TISSUE CULTURE IV. FURTHER OBSERVATIONS ON VIRUS PROPAGATION IN HUMAN TISSUES WITH A SYNTHETIC NUTRIENT MEDIUM

1953 ◽  
Vol 31 (1) ◽  
pp. 64-74 ◽  
Author(s):  
A. E. Franklin ◽  
D. Duncan ◽  
W. Wood ◽  
A. J. Rhodes
Keyword(s):  
Tissue Culture ◽  
Human Tissue ◽  
Nutrient Medium ◽  
Glucose Utilization ◽  
Embryonic Brain ◽  
Human Tissues ◽  
Human Embryonic Kidney ◽  
Virus Propagation ◽  
Poliomyelitis Virus ◽  
Synthetic Nutrient Medium

This paper presents further observations on the propagation of the Lansing strain of poliomyelitis virus in Maitland-type cultures of human tissue derived from embryonic brain and cord and from kidney, in a synthetic nutrient medium. The survival time of cultures of human embryonic brain and cord was previously found to be over 70 days, and we now report that cultures of human embryonic kidney have survived for over 100 days. Virus has been detected in the supernatant fluids of cultures of brain and cord for more than 60 days, and of kidney for more than 100 days. Virus titers of more than 10−3.0 have been obtained in cultures of human embryonic kidney. Human tonsillar tissue has survived for more than 50 days in cultures, and virus has been detected in the supernatant fluids for more than 40 days. Studies on glucose utilization have been of value in estimating the level of metabolism of these tissues.

CULTIVATION OF POLIOMYELITIS VIRUS IN TISSUE CULTURE V. OBSERVATIONS ON VIRUS PROPAGATION IN CERTAIN ANIMAL TISSUES WITH A SYNTHETIC NUTRIENT MEDIUM

1953 ◽  
Vol 31 (1) ◽  
pp. 75-83 ◽  
Author(s):  
D. Duncan ◽  
A. E. Franklin ◽  
W. Wood ◽  
A. J. Rhodes
Keyword(s):  
Tissue Culture ◽  
Rhesus Monkeys ◽  
Synthetic Medium ◽  
Tissue Cultures ◽  
Animal Tissues ◽  
Virus Propagation ◽  
Virus Growth ◽  
Poliomyelitis Virus ◽  
The Brain ◽  
Synthetic Nutrient Medium

Further observations have been made on the propagation of Lansing poliomyelitis virus in tissue cultures. It has been observed that tissues derived from several organs of rhesus monkeys will support virus growth in tissue cultures in Erlenmeyer flasks with a synthetic medium as the source of nutrient. Cultures of tissues from monkey testis, lung, kidney, and gut have survived for long periods, and virus has been regularly recovered even from fluids removed from cultures after as late as 125 days. Cultures of tissues from monkey brain and cord, and muscle, did not survive as long, and less virus was demonstrated in the supernatant fluids. Muscle from the diaphragm did not appear to support growth. Cultures of tissues from the brain, kidney, and lung of beef embryos survived for long periods, but no virus was found in any of the culture fluids.

Cultivation of Poliomyelitis Virus in Tissue Culture III. Synthetic Medium in Roller Tube Cultures.

1952 ◽  
Vol 81 (2) ◽  
pp. 434-438 ◽  
Author(s):  
W. Wood ◽  
A. E. Franklin ◽  
E. M. Clark ◽  
D. Duncan ◽  
A. J. Rhodes
Keyword(s):  
Tissue Culture ◽  
Synthetic Medium ◽  
Poliomyelitis Virus ◽  
Roller Tube

scholarly journals Effect of Force 6® Poultry on Infectious Bursal disease Virus in Vitro

2015 ◽  
Vol 39 (2) ◽  
pp. 87-90
Author(s):  
Amjed H. . Ulaiwi
Keyword(s):  
Tissue Culture ◽  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Chicken Embryo Fibroblast ◽  
Virus Titer ◽  
Infectious Bursal Disease ◽  
Virus Growth ◽  
Bursal Disease ◽  
And Control

     The aim of this study was to investigate the effect of force 6 poultry (conc.) in Log. of infectious bursal disease Virus on tissue culture and Virus, on Virus alone  and   on tissue culture alone. Different concentrations (0.5, 12.5, 25 and 50 µg/ml) were used to consider the anti-viral activity. The result showed effect of force 6® poultry (conc.) in Log. of Infectious Bursal Disease Virus. On tissue culture and Virus, the results revealed higher value with more significant (P˂0.05) differences at concentration (0.5 µg/ml) than other concentrations and control; On Virus alone, it showed more significant (P˂0.05) differences at concentration (0.5 µg/ml) than concentration (50 µg/ml) which showed less value of Virus growth. And on tissue culture alone (chicken embryo fibroblast) it showed lesser value and significant (P˂0.05) differences than other concentrations. In conclusion, the main changes in tissue culture explained at concentration (0.5, 25 and 50 µg/ml) but not (12.5 µg/ml and control). This group also were more affected on Virus titer when compared with other than two groups (tissue culture and Virus and Virus alone).

Adipose cell morphology and control of lipolysis in a patient with partial lipodystrophy

Metabolism ◽  
1979 ◽  
Vol 28 (5) ◽  
pp. 519-526 ◽  
Author(s):  
Robert S. Bernstein ◽  
Richard N. Pierson ◽  
Steven F. Ryan ◽  
Stephen B. Crespin
Keyword(s):  
Cell Morphology ◽  
Partial Lipodystrophy ◽  
Adipose Cell ◽  
And Control

scholarly journals The behavioral effects of antibiotic treatment on the snailBiomphalaria glabrata

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e4171 ◽  
Author(s):  
Euan R.O. Allan ◽  
Michael S. Blouin
Keyword(s):  
Tissue Culture ◽  
Intermediate Host ◽  
Antibiotic Treatment ◽  
New World ◽  
Biomphalaria Glabrata ◽  
Behavioral Effects ◽  
Neglected Tropical Disease ◽  
Intermediate Hosts ◽  
And Control ◽  
Main Model

Schistosomiasis is a detrimental neglected tropical disease that is transmitted by Planorbid snails. Understanding the transmission and control of this disease requires an extensive understanding of these intermediate hosts, which is only achieved by the effective rearing and study of species such asBiomphalaria glabrata. This species is the intermediate host forSchistosoma mansoniin the New World, and is also the main model for studying schistosomes in mollusks. Antibiotics are used routinely inB. glabratatissue culture, and occasionally on live snails. Here we show that standard doses of three common antibiotics (penicillin, streptomycin and gentamicin) drastically diminish the activity of healthyB. glabrata, but that treated snails recover rapidly when placed in fresh water. Ampicillin treated snails did not show altered activity. We suggest that researchers keep these apparent toxicities in mind if a need for antibiotic treatment of live Planorbid snails arises.

Production and control of extracellular enzymes in Micrococcus sodonensis

1974 ◽  
Vol 20 (1) ◽  
pp. 81-90 ◽  
Author(s):  
Cecily Mills ◽  
J. N. Campbell
Keyword(s):  
Alkaline Phosphatase ◽  
Inorganic Phosphate ◽  
Culture Medium ◽  
Extracellular Enzymes ◽  
Synthetic Medium ◽  
Culture Conditions ◽  
The Other ◽  
Organic Nutrients ◽  
And Control ◽  
Logarithmic Growth

Micrococcus sodonensis has been shown to produce several extracellular enzymes: an alkaline phosphatase, at least two forms of phosphodiesterase, a 5′-nucleotidase, and an alkaline proteinase. The quantitative release of these enzymes into the culture medium during logarithmic growth under all the various culture conditions tested indicates that these enzymes are truly extracellular in nature. Inorganic phosphate repressed the production of the alkaline phosphatase in synthetic as well as in complex media, whereas, the repression of the production of active diesterase and 5′-nucleotidase by inorganic phosphate was partly reversed by the addition of supplemental organic nutrients to the culture medium. Proteinase production was independent of the culture conditions used. A mutant strain of M. sodonensis with an altered production of diesterase was obtained; the other extracellular enzymes were unaffected. These results suggest that the extracellular enzymes of M. sodonensis are not produced in a pleiotropic fashion since the level of one of the enzymes can be changed without affecting a corresponding change in the levels of the other enzymes. An extracellular high molecular weight carbohydrate fraction was shown to be produced by M. sodonensis in synthetic medium. The fraction was also shown to contain glycoprotein.

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