scholarly journals Effect of Force 6® Poultry on Infectious Bursal disease Virus in Vitro

2015 ◽  
Vol 39 (2) ◽  
pp. 87-90
Author(s):  
Amjed H. . Ulaiwi
Keyword(s):  
Tissue Culture ◽  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Chicken Embryo Fibroblast ◽  
Virus Titer ◽  
Infectious Bursal Disease ◽  
Virus Growth ◽  
Bursal Disease ◽  
And Control

     The aim of this study was to investigate the effect of force 6 poultry (conc.) in Log. of infectious bursal disease Virus on tissue culture and Virus, on Virus alone  and   on tissue culture alone. Different concentrations (0.5, 12.5, 25 and 50 µg/ml) were used to consider the anti-viral activity. The result showed effect of force 6® poultry (conc.) in Log. of Infectious Bursal Disease Virus. On tissue culture and Virus, the results revealed higher value with more significant (P˂0.05) differences at concentration (0.5 µg/ml) than other concentrations and control; On Virus alone, it showed more significant (P˂0.05) differences at concentration (0.5 µg/ml) than concentration (50 µg/ml) which showed less value of Virus growth. And on tissue culture alone (chicken embryo fibroblast) it showed lesser value and significant (P˂0.05) differences than other concentrations. In conclusion, the main changes in tissue culture explained at concentration (0.5, 25 and 50 µg/ml) but not (12.5 µg/ml and control). This group also were more affected on Virus titer when compared with other than two groups (tissue culture and Virus and Virus alone).

scholarly journals Immunogenicity and Efficacy Evaluation of Vero Cell-Adapted Infectious Bursal Disease Virus LC-75 Vaccine Strain in Ethiopia 

2021 ◽  
Author(s):  
Wakjira Kebebe ◽  
Molalegne Bitew ◽  
Fufa Dawo ◽  
Bedaso Mammo ◽  
Hawa Mohammed ◽  
...  
Keyword(s):  
Vero Cell ◽  
Disease Virus ◽  
Vaccine Strain ◽  
Infectious Bursal Disease Virus ◽  
Chicken Embryo Fibroblast ◽  
Vero Cells ◽  
Infectious Bursal Disease ◽  
Efficacy Evaluation ◽  
Viral Pathogen ◽  
Bursal Disease

Abstract Background: Infectious bursal disease virus (IBDV) is an avian viral pathogen that causes infectious bursal disease (IBD) of chickens. The disease is endemic in Ethiopia since 2002 and vaccination is the major means of disease prevention and control. IBD vaccine is produced in Ethiopia using primary chicken embryo fibroblast (CEF) cell; which is time-consuming, laborious, and uneconomical. The present study was carried out to develop cell-based IBDV LC-75 vaccine using Vero cells, and to evaluate the immunogenicity and protection level.Results: Identity of the vaccine seed was confirmed using gene-specific primers using reverse transcription polymerase chain reaction. Confluent monolayer of Vero cells was infected with vaccine virus and serial passage continued till passage ten. Characteristic virus induced cytopathic effect was observed starting from passage 2 on the third day post-infection. The infectious titer of adapted virus showed a linear increment along the passage level. Virus induced specific antibody was determined using indirect ELISA after vaccination of 14 days old chicks through ocular route. Accordingly, the antibody titer measured from Vero cells vaccinated chicks revealed similar level with the currently available CEF cell-based vaccine. Chicks vaccinated with Vero cell adapted virus showed complete protection against very virulent IBDV, while unvaccinated group had 60% morbidity and 25% mortality.Conclusions: The IBDV vaccine strain well adapted on Vero cells and found to be immunogenic induces antibodies development and successfully protects chicks against challenging with the circulating field IBDV isolate. Hence, it is recommended to produce IBD vaccine using Vero cell culture with enough quantity to conquer the limitations using CEF cells and thus to vaccinate chicks to protect against IBDV infection.

scholarly journals Alteration of amino acids in VP2 of very virulent infectious bursal disease virus results in tissue culture adaptation and attenuation in chickens

2002 ◽  
Vol 83 (1) ◽  
pp. 121-129 ◽  
Author(s):  
A. A. W. M. van Loon ◽  
N. de Haas ◽  
I. Zeyda ◽  
E. Mundt
Keyword(s):  
Amino Acids ◽  
Tissue Culture ◽  
Amino Acid ◽  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Site Directed Mutagenesis ◽  
Infectious Bursal Disease ◽  
Single Amino Acid ◽  
Culture Adaptation ◽  
Bursal Disease

Reverse genetics technology offers the possibility to study the influence of particular amino acids of infectious bursal disease virus (IBDV) on adaptation to tissue culture. Genomic segments A and B of the very virulent (vv) IBDV field strain UK661 were completely cloned and sequenced, and the strain was rescued from full-length cDNA copies of both segments (UK661rev). Using site-directed mutagenesis, alteration of a single amino acid in the segment A-encoded VP2 (A284T) resulted in a limited capacity of UK661 to replicate in tissue culture. Additional alteration of a second amino acid (Q253H) increased replication efficiency in tissue culture. The second mutant (UK661-Q253H-A284T) was used to infect chickens and results were compared with UK661 and UK661rev. Whereas UK661 and UK661rev induced 100% morbidity and 50–80% mortality, UK661-Q253H-A284T proved to be strikingly attenuated, producing neither morbidity nor mortality. Moreover, UK661-Q253H-A284T-infected animals were protected from challenge infection. Thus, alteration of two specific amino acids in the VP2 region of IBDV resulted in tissue culture adaptation and attenuation in chickens of vvIBDV. The data demonstrate that VP2 plays a decisive role in pathogenicity of IBDV.

scholarly journals Complete Genome Sequence of a Chicken Embryo Fibroblast-Adapted Attenuated Infectious Bursal Disease Virus Isolate from India

2016 ◽  
Vol 4 (3) ◽  
Author(s):  
T. M. A. Senthilkumar ◽  
C. V. Priyadharsini ◽  
P. Raja ◽  
K. Kumanan
Keyword(s):  
Disease Virus ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Infectious Bursal Disease Virus ◽  
Chicken Embryo Fibroblast ◽  
Infectious Bursal Disease ◽  
Avian Pathogen ◽  
Bursal Disease ◽  
Commercial Broiler

Infectious bursal disease virus is an avian pathogen that causes huge morbidity and mortality in the poultry sector all over the world. Here, we report the full-length genome sequence of an Indian strain, MB11/ABT/MVC/2016, isolated from a commercial broiler flock. This is a first report of a complete genome sequence of infectious bursal disease virus from India.

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