Genetic variation within Pseudoterranova decipiens (Nematoda: Ascaridoidea) from Canadian Atlantic marine fishes and seals: characterization by RFLP analysis of genomic DNA

1996 ◽  
Vol 53 (2) ◽  
pp. 333-341 ◽  
Author(s):  
J Brattey ◽  
W S Davidson
Keyword(s):  
Genetic Variation ◽  
Genomic Dna ◽  
Rflp Analysis ◽  
Marine Fishes ◽  
Pseudoterranova Decipiens

Species diversity and genetic variation of caligids (Copepoda: Siphonostomatoida) infecting the marine fishes of Kerala coast, India

2021 ◽  
Vol 52 (1) ◽  
Author(s):  
MV Nikhila Reshmi ◽  
K Rijin ◽  
OK Drisya ◽  
TA Jose Priya ◽  
Sudha Kappalli
Keyword(s):  
Genetic Variation ◽  
Species Diversity ◽  
Marine Fishes ◽  
Kerala Coast

Assessment of the degree of genetic variation in beet based on RFLP analysis and the taxonomy of Beta

Euphytica ◽  
1991 ◽  
Vol 55 (1) ◽  
pp. 1-6 ◽  
Author(s):  
G. Mita ◽  
M. Dani ◽  
P. Casciari ◽  
A. Pasquali ◽  
E. Selva ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Rflp Analysis

Characterization of six wheat × Thinopyrum intermedium derivatives by GISH, RFLP, and multicolor GISH

Genome ◽  
2003 ◽  
Vol 46 (3) ◽  
pp. 490-495 ◽  
Author(s):  
F P Han ◽  
G Fedak ◽  
A Benabdelmouna ◽  
K Armstrong ◽  
T Ouellet
Keyword(s):  
Genomic Dna ◽  
Aegilops Tauschii ◽  
Rflp Analysis ◽  
Aegilops Speltoides ◽  
Thinopyrum Intermedium ◽  
B Genome ◽  
A Genome ◽  
Genetic Stocks ◽  
Disomic Addition Lines ◽  
Thinopyrum Elongatum

Restriction fragment length polymorphism (RFLP) analysis and multicolor genomic in situ hybridization (GISH) are useful tools to precisely characterize genetic stocks derived from crosses of wheat (Triticum aestivum) with Thinopyrum intermedium and Thinopyrum elongatum. The wheat × Th. intermedium derived stocks designated Z1, Z2, Z3, Z4, Z5, and Z6 were initially screened by multicolor GISH using Aegilops speltoides genomic DNA for blocking and various combinations of genomic DNA from Th. intermedium, Triticum urartu, and Aegilops tauschii for probes. The probing (GISH) results indicated that lines Z1 and Z3 were alien disomic addition lines with chromosome numbers of 2n = 44. Z2 was a substitution line in which chromosome 2D was substituted by a pair of Th. intermedium chromosomes; this was confirmed by RFLP and muticolour GISH. Z4 (2n = 44) contained two pairs of wheat – Th. intermedium translocated chromosomes; one pair involved A-genome chromosomes, the other involved D- and A-genome chromosomes. Z5 (2n = 44) contained one pair of wheat – Th. intermedium translocated chromosomes involving the D- and A-genome chromosomes of wheat. Z6 (2n = 44) contained one pair of chromosomes derived from Th. intermedium plus another pair of translocated chromosomes involving B-genome chromosomes of wheat. Line Z2 was of special interest because it has some resistance to infection by Fusarium graminearum.Key words: wheat, Thinopyrum intermedium, addition, substitution, and translocation lines, GISH, multicolor GISH, RFLP.

RFLP Analysis of Mitochondrial DNA to Evaluate Genetic Variation in Striped Red Mullet (Mullus surmuletus L.) and Red Mullet (Mullus barbatus L.) Populations

2001 ◽  
Vol 3 (3) ◽  
pp. 264-274 ◽  
Author(s):  
Zissis Mamuris ◽  
Costas Stamatis ◽  
Katerina A. Moutou ◽  
Apostolos P. Apostolidis ◽  
Costas Triantaphyllidis
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Variation ◽  
Rflp Analysis ◽  
Red Mullet ◽  
Mullus Barbatus ◽  
Mullus Surmuletus

scholarly journals High degree of genetic variation inProchlorococcus(Prochlorophyta) revealed by RFLP analysis

1996 ◽  
Vol 31 (1) ◽  
pp. 1-9 ◽  
Author(s):  
David J. Scanlan ◽  
Wolfgang R. Hess ◽  
Frédéric Partensky ◽  
Julie Newman ◽  
Daniel Vaulot
Keyword(s):  
Genetic Variation ◽  
Rflp Analysis ◽  
High Degree

scholarly journals Genetic variation detected by quantitative analysis of end-labeled genomic DNA fragments.

1994 ◽  
Vol 91 (19) ◽  
pp. 9052-9056 ◽  
Author(s):  
J. Asakawa ◽  
R. Kuick ◽  
J. V. Neel ◽  
M. Kodaira ◽  
C. Satoh ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Quantitative Analysis ◽  
Genomic Dna ◽  
Dna Fragments

scholarly journals 469 PB 336 INTER- AND INTRA-LINE GENOTYPIC VARIATION OF U. S. COLLARD CULTIVARS AND LANDRACES DETERMINED BY RAPD ANALYSIS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 498d-498
Author(s):  
Mark W. Farnham
Keyword(s):  
Genetic Variation ◽  
Genomic Dna ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
Genotypic Variation ◽  
Land Race ◽  
Commercial Cultivars ◽  
Polymorphic Bands ◽  
Line Analysis ◽  
Brassica Oleracea L

Collard (Brassica oleracea L. var. acephala) is an important vegetable the southeastern U. S. There are few (about 10) commercial cultivars, half being open-pollinating (OP) lines, the remainder more recent F1 hybrids. There is a potential untapped B. oleracea germplasm pool in the form of collard landraces perpetuated by southeastern gardeners and farmers. To determine the amount of genetic variation among cultivars and also whether landraces represent unique genotypes, ten cultivars and eight lines or landraces were evaluated using RAPD analysis. Decamer primers were used to amplify total genomic DNA and to differentiate collard lines and other B. oleracea crop cultivars. Additionally, individuals of an OP collard cultivar and a land-race were analyzed to evaluate intra-line variation. Virtually all primers detected polymorphic bands among lines although some identified considerably more variants. Intra-line analysis indicated that OP lines are genetically broad-based populations. Many unique RAPD markers were identified in landraces indicating that the lines represent unique genotypes and that further line collection is warranted.

scholarly journals Genetic diversity among forty coffee varieties assessed by RAPD markers associated with restriction digestion

2005 ◽  
Vol 48 (4) ◽  
pp. 511-521 ◽  
Author(s):  
Leandro Eugênio Cardamoni Diniz ◽  
Claudete de Fátima Ruas ◽  
Valdemar de Paula Carvalho ◽  
Fabrício Medeiros Torres ◽  
Eduardo Augusto Ruas ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Genetic Variability ◽  
Clustering Analysis ◽  
Genomic Dna ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Pcr Amplification ◽  
Restriction Digestion ◽  
Agronomic Features

The genetic variability of 40 accessions of_C. arabica was evaluated using a combination of the random amplified polymorphic DNA (RAPD) technique and restriction digestion of genomic DNA. The genetic variability and the relatedness among all accessions were initially evaluated using 195 RAPD primers which revealed a very low level of genetic variation. To improve the efficiency in the detection of polymorphism, the genomic DNA of all accessions were submitted to digestion with restriction endonucleases prior to PCR amplification. A total of 24 primers combined with restriction digestion of DNA rendered 318 bands, of which 266 (83.65%) were polymorphic. The associations among genotypes were estimated using UPGMA-clustering analysis. The accessions were properly clustered according to pedigree and agronomic features. The ability to distinguish among coffee accessions was greater for RAPD plus restriction digestion than for RAPD alone, providing evidences that the combination of the techniques was very efficient for the estimation of genetic relationship among_C. arabica genotypes.

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