High degree of genetic variation inProchlorococcus(Prochlorophyta) revealed by RFLP analysis

SummaryA rare recombinant within the multigenic Hor 2 locus of barley was detected by SD-PAGE of hordein fractions from F2 grain from the cross Bomi × P12/3. Analysis of a homozygous F4 line by 2-D IEF/SDS-PAGE showed that recombination between the class I/II and class III subfamilies of genes had occurred, indicating that they are spatially separate within the Hor 2 locus. RFLP analysis using a B hordein-related cDNA clone confirmed that recombination had occurred, while similar analysis using a genomic clone related to γ-type hordein (encoded by the Hor 5/HrdF locus) indicated the order of the two subfamilies of genes on the short arm of chromosome 5, the class I/II genes being closer to the centromere. The results are consistent with the origin of the B hordein gene family from initial duplication of a single ancestral gene to give two genes which diverged to become the ancestors of the class I/II and class III subfamilies. Subsequent cycles of duplication and divergence have resulted in the present high degree of polymorphism.

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Rapd variation of late-maturing sorghum [ Sorghum bicolor (L.) Moench] landraces from Ethiopia

Acta Agronomica Hungarica ◽

10.1556/aagr.55.2007.3.14 ◽

2007 ◽

Vol 55 (3) ◽

pp. 375-382 ◽

Cited By ~ 3

Author(s):

S. Mamo ◽

A. Ayana ◽

T. Tesso

Keyword(s):

Genetic Variation ◽

Genetic Distance ◽

Sorghum Bicolor ◽

Rapd Markers ◽

Diversity Index ◽

Random Amplified Polymorphic Dna ◽

The Mean ◽

Polymorphic Bands ◽

High Degree ◽

Sorghum Bicolor L

A study on the extent and pattern of genetic variability in late-maturing sorghum [ Sorghum bicolor (L.) Moench] landraces collected from the Wello and Hararge areas of Ethiopia was conducted using random amplified polymorphic DNA (RAPD) markers for 70 individuals representing 14 populations. Four oligonucleotide primers generated a total of 55 polymorphic bands with 13–19 bands per primer and a mean of 16 bands across the 70 individuals. The value of the Shannon diversity index among the populations (0.26) and between the two regions (0.24) was low to moderate, despite the high degree of polymorphic bands per primer. The mean genetic distance (0.25) between the populations was found to be low. The low genetic variation may be due to the reduced population size of late-maturing sorghum landraces in the two regions of Ethiopia because of farmers’ decisions in the process of planting, managing, harvesting and processing their crops. Partitioning of the genetic variation into variation between and within the population revealed that 92.9% and 7.10% of the variation was found to be between and within the populations, respectively. Cluster analysis of genetic distance estimates further confirmed a low level of differentiation in late-maturing sorghum populations both between and within the regions. The implications of the results for genetic conservation purposes are discussed.

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RFLP Analysis of Mitochondrial DNA to Evaluate Genetic Variation in Striped Red Mullet (Mullus surmuletus L.) and Red Mullet (Mullus barbatus L.) Populations

Marine Biotechnology ◽

10.1007/s101260000075 ◽

2001 ◽

Vol 3 (3) ◽

pp. 264-274 ◽

Cited By ~ 12

Author(s):

Zissis Mamuris ◽

Costas Stamatis ◽

Katerina A. Moutou ◽

Apostolos P. Apostolidis ◽

Costas Triantaphyllidis

Keyword(s):

Mitochondrial Dna ◽

Genetic Variation ◽

Rflp Analysis ◽

Red Mullet ◽

Mullus Barbatus ◽

Mullus Surmuletus

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Genetic variation between and within two populations of Rhabdocline pseudotsugae in Germany

Canadian Journal of Forest Research ◽

10.1139/cjfr-2015-0430 ◽

2016 ◽

Vol 46 (5) ◽

pp. 716-724 ◽

Cited By ~ 1

Author(s):

Kristin Morgenstern ◽

Jens-Ulrich Polster ◽

Doris Krabel

Keyword(s):

Genetic Diversity ◽

Genetic Variation ◽

Spatial Distribution ◽

Pseudotsuga Menziesii ◽

Early Stage ◽

Start Codon ◽

Fruiting Bodies ◽

Clear Differentiation ◽

High Degree ◽

Two Populations

Knowledge of the ecology and biology of Rhabdocline pseudotsugae Sydow is still at a very early stage. Recent results indicate the existence of an endophytic stage of the fungus in the form of symptomless infections in various Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) tissue types. This study represents the first description of genetic variation between and within two populations of R. pseudotsugae. Needles featuring fruiting bodies of R. pseudotsugae were collected from sample areas in Saxony and North Rhine-Westphalia (in Germany), with different fungal genotypes distinguished using the start codon targeted polymorphism (SCoT) and branch point signal sequences (BPS) techniques. Five of the 20 primers tested could be selected for further investigation. A total of 349 fragments were amplified at an average of 69.8 fragments per primer. Results showed the two sample areas to exhibit a high degree of both genetic variability and genetic diversity. A clear differentiation between the sample areas was not observed. There was nevertheless a clear connection between the genetic distance and spatial distribution of the fruiting bodies of R. pseudotsugae within the sample areas themselves.

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Analisis Keragaman Genetik Acremonium yang Berasosiasi dengan Tanaman Gaharu Menggunakan Teknik Random Amplified Polymorphic DNA (RAPD)

Jurnal AgroBiogen ◽

10.21082/jbio.v5n2.2009.p65-70 ◽

2016 ◽

Vol 5 (2) ◽

pp. 65

Author(s):

Dewi Rahmawati ◽

Nurita Toruan-Mathius

Keyword(s):

Cluster Analysis ◽

Genetic Variation ◽

Genetic Similarity ◽

Random Amplified Polymorphic Dna ◽

Economic Value ◽

Similarity Index ◽

Similarity Coefficients ◽

West Sumatra ◽

High Degree ◽

Geographical Locations

Agarwood or gaharu is a plant that has a high economic value in Asia, due to its use for production of incense and traditional medicines. The agarwood formation occurs in the trunk and roots of trees that have been infected by a fungus, such as Acremonium spp. Various fungi were associated with the agarwood formation. Acremonium is generally considered as highly polyphyletic, contains distantly related fungi. A study was done to identify genetic diversities in 10 isolates of Acremonium spp. from four different areas in Indonesia that are associated with Aquilaria and Gyrinops verstigii using the Random Amplified Polymorphic DNA (RAPD) technique. Eight RAPD primers, i.e., OPA 02, OPB 04, OPB 07, OPB 17, OPC 11, OPD 03, OPD 05, and OPE 07 were used in the analyses. The results indicated that similarity index values of the genetic variation ranged from 0.21 to 0.97. Based on the Nei and Li’s similarity coefficients, these values indicating the presence of high degree of genetic variability. The lowest degree of genetic similarity were found between isolates F (Acremonium spp., which is associated with G. verstigii from Mataram, Nusa Tenggara Barat), and LM2 from south coastal area of West Sumatra. The highest genetic similarity value (0.97) was found between isolates Sr2 and Sr4 from Sorong, Papua. Results from the cluster analysis indicated that the isolates could be grouped into two major clusters that were associated with their geographical locations.

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