Identification of RFLP markers for resistance to wheat spindle streak mosaic bymovirus (WSSMV) disease

Wheat spindle streak mosaic bymovirus (WSSMV) causes an economically important disease of winter wheat in Europe and North America. Artificial inoculation with this virus to identify resistant wheat genotypes is difficult. This study was conducted to identify restriction fragment length polymorphism (RFLP) markers associated with resistance to this disease. A population, consisting of 104 F5 recombinant inbred lines from a cross between hexaploid Triticum aestivum cultivars 'Geneva' (resistant) and 'Augusta' (susceptible), was evaluated for WSSMV symptoms under field conditions for four years. Two linked markers on the long arm of chromosome 2D, Xbcd1095 and Xcdo373, were determined to be associated with WSSMV resistance by bulked segregant analysis of the 10 most resistant and 10 most susceptible lines. Marker Xcdo373 accounted for 79% and Xbcd1095 for 73% of the phenotypic variation. Our results suggest that resistance to WSSMV in this population is qualitative in nature and is controlled by few genes. These markers should be useful in the development of wheat cultivars resistant to WSSMV and perhaps also to wheat yellow mosaic bymovirus (WYMV).Key words: wheat, wheat spindle streak mosaic virus (WSSMV), RFLP markers, resistance, bymovirus, wheat yellow mosaic virus, barley yellow mosaic virus.

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Biological and Genetic Diversity of Wheat yellow mosaic virus (Genus Bymovirus)

Phytopathology ◽

10.1094/phyto-06-13-0150-r ◽

2014 ◽

Vol 104 (3) ◽

pp. 313-319 ◽

Cited By ~ 12

Author(s):

Takehiro Ohki ◽

Osamu Netsu ◽

Hisayo Kojima ◽

Junichi Sakai ◽

Masatoshi Onuki ◽

...

Keyword(s):

Genetic Diversity ◽

Amino Acid ◽

Mosaic Virus ◽

Fragment Length Polymorphism ◽

Wheat Cultivar ◽

Yellow Mosaic Virus ◽

Polymorphism Analysis ◽

Wheat Yellow Mosaic Virus ◽

Northern Areas ◽

Polymerase Chain

The biological and genetic diversity of Wheat yellow mosaic virus (WYMV) isolates in Japan was characterized. On the basis of wheat cultivar reactions, 14 WYMV isolates from various places were classified into pathotypes I, II, or III. These were distributed in central, northern, and southern areas of Japan, respectively. WYMV isolates comprised three genotypes (A, A′ and B) based on amino acid differences in RNA1 and two genotypes (a and b) based on amino acid differences in RNA2. A correlation was found between the WYMV RNA1-based genotype and pathotype, suggesting that factors associated with pathogenicity map to RNA1. Genotype Aa and A′a were distributed mainly in the central to southern areas of Japan, and genotype Bb was found in northern areas of Japan, as shown by reverse-transcription polymerase chain reaction restriction fragment length polymorphism analysis. Chinese isolates YA and YZ were closely related to genotypes Bb and Aa, respectively. Wheat was introduced from China to Japan in the 4th and 5th centuries, and the two genotypes of WYMV might also have been introduced with the crop from China and later adapted to local wheat cultivars in Japan.

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Comparison and differentiation of Wheat Yellow Mosaic Virus(WYMV), Wheat Spindle Streak Mosaic Virus (WSSMV) and Barley Yellow Mosaic Virus (BaYMV) isolates using WYMV monoclonal antibodies

European Journal of Plant Pathology ◽

10.1007/bf01877967 ◽

1996 ◽

Vol 102 (3) ◽

pp. 283-292 ◽

Cited By ~ 10

Author(s):

Djabbar Hariri ◽

Thierry Delaunay ◽

Laure Gomes ◽

Sophie Filleur ◽

Christelle Plovie ◽

...

Keyword(s):

Monoclonal Antibodies ◽

Mosaic Virus ◽

Yellow Mosaic Virus ◽

Wheat Yellow Mosaic Virus ◽

Barley Yellow Mosaic Virus

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Association of VPg and eIF4E in the host tropism at the cellular level of Barley yellow mosaic virus and Wheat yellow mosaic virus in the genus Bymovirus

Virology ◽

10.1016/j.virol.2014.12.010 ◽

2015 ◽

Vol 476 ◽

pp. 159-167 ◽

Cited By ~ 5

Author(s):

Huangai Li ◽

Yukio Shirako

Keyword(s):

Mosaic Virus ◽

Cellular Level ◽

Yellow Mosaic Virus ◽

Wheat Yellow Mosaic Virus ◽

Host Tropism ◽

Barley Yellow Mosaic Virus

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Genome-Wide Identification and Expression Analysis of the Histone Deacetylase Gene Family in Wheat (Triticum aestivum L.)

Plants ◽

10.3390/plants10010019 ◽

2020 ◽

Vol 10 (1) ◽

pp. 19

Author(s):

Peng Jin ◽

Shiqi Gao ◽

Long He ◽

Miaoze Xu ◽

Tianye Zhang ◽

...

Keyword(s):

Abiotic Stress ◽

Gene Family ◽

Mosaic Virus ◽

Stress Responses ◽

Viral Infections ◽

Phylogenetic Analyses ◽

Plant Viruses ◽

Gene Family Evolution ◽

Yellow Mosaic Virus ◽

Wheat Yellow Mosaic Virus

Histone acetylation is a dynamic modification process co-regulated by histone acetyltransferases (HATs) and histone deacetylases (HDACs). Although HDACs play vital roles in abiotic or biotic stress responses, their members in Triticumaestivum and their response to plant viruses remain unknown. Here, we identified and characterized 49 T. aestivumHDACs (TaHDACs) at the whole-genome level. Based on phylogenetic analyses, TaHDACs could be divided into 5 clades, and their protein spatial structure was integral and conserved. Chromosomal location and synteny analyses showed that TaHDACs were widely distributed on wheat chromosomes, and gene duplication has accelerated the TaHDAC gene family evolution. The cis-acting element analysis indicated that TaHDACs were involved in hormone response, light response, abiotic stress, growth, and development. Heatmaps analysis of RNA-sequencing data showed that TaHDAC genes were involved in biotic or abiotic stress response. Selected TaHDACs were differentially expressed in diverse tissues or under varying temperature conditions. All selected TaHDACs were significantly upregulated following infection with the barley stripe mosaic virus (BSMV), Chinese wheat mosaic virus (CWMV), and wheat yellow mosaic virus (WYMV), suggesting their involvement in response to viral infections. Furthermore, TaSRT1-silenced contributed to increasing wheat resistance against CWMV infection. In summary, these findings could help deepen the understanding of the structure and characteristics of the HDAC gene family in wheat and lay the foundation for exploring the function of TaHDACs in plants resistant to viral infections.

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