Molecular cytogenetic identification of nullisomy 5B induced homoeologous recombination between wheat chromosome 5D and barley chromosome 5H

Genome ◽  
2005 ◽  
Vol 48 (1) ◽  
pp. 115-124 ◽  
Author(s):  
Shin Taketa ◽  
Takaya Awayama ◽  
Masahiko Ichii ◽  
Makoto Sunakawa ◽  
Tomoko Kawahara ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Hordeum Vulgare ◽  
Wheat Chromosome ◽  
The Other ◽  
Homoeologous Recombination ◽  
Wheat Group ◽  
Self Fertilization ◽  
Homoeologous Relationships ◽  
Group 5

Chromosome 5H of Hordeum vulgare 'New Golden' (NG) carries a gene(s) that accelerates heading in a wheat background. To introduce the early heading gene(s) of NG barley into the wheat genome, we attempted to induce homoeologous recombination between wheat and NG 5H chromosomes by 5B nullisomy. A nullisomic 5B, trisomic 5A, monosomic 5H plant (2n = 42) was produced from systematic crosses between aneuploid stocks of wheat group 5 chromosomes. A total of 656 F2 plants produced by self-fertilization were screened for recombinants by a PCR assay with 3 5H-specific amplicon markers. Twelve plants (1.8%) were selected as putative wheat–barley 5H recombinants. Five of them were inviable or sterile and the remaining 7 were fertile and subjected to the progeny test. Cytological analyses using fluorescence in situ hybridization and C-banding revealed that 6 of the 7 progeny lines are true homoeologous recombinants between the long arms of chromosomes 5D and 5H, but that the other one was not a recombinant having an aberrant barley telosome. The 6 cytologically confirmed recombinant lines included only 2 types (3 lines each), which were reciprocal products derived from exchanges at the same distal interval defined by two flanking markers. One type had a small 5HL segment translocated to the 5DL terminal, and the other type had a small terminal 5DL segment translocated to the 5HL terminal. In the latter type, the physical length of translocated barley segments slightly differed among lines. Homoeologous recombinants obtained in this study should be useful for further chromosome manipulation to introgress a small interstitial 5HL chromosome segment with the early heading gene(s) to wheat. Preferential occurrence of restricted types of recombinants is discussed in relation to homoeologous relationships between wheat and barley chromosomes.Key words: genomic in situ hybridization, homoeologous pairing, Hordeum vulgare, introgression, recombinant, Triticum aestivum.

scholarly journals Brief communication. In situ hybridization mapping of genes in Hordeum vulgare L.

1998 ◽  
Vol 89 (4) ◽  
pp. 366-370 ◽  
Author(s):  
G Butnaru ◽  
J Chen ◽  
P Goicoechea ◽  
JP Gustafson
Keyword(s):  
In Situ Hybridization ◽  
Hordeum Vulgare ◽  
Hordeum Vulgare L ◽  
Hybridization Mapping

Physical mapping of barley genes using an ultrasensitive fluorescence in situ hybridization technique

Genome ◽  
2004 ◽  
Vol 47 (1) ◽  
pp. 179-189 ◽  
Author(s):  
J L Stephens ◽  
S E Brown ◽  
N L.V Lapitan ◽  
D L Knudson
Keyword(s):  
In Situ Hybridization ◽  
Hordeum Vulgare ◽  
Fluorescence In Situ Hybridization ◽  
Linkage Map ◽  
Physical Mapping ◽  
Physical Map ◽  
Primary Objective ◽  
Hybridization Technique ◽  
Hordeum Vulgare L

The primary objective of this study was to elucidate gene organization and to integrate the genetic linkage map for barley (Hordeum vulgare L.) with a physical map using ultrasensitive fluorescence in situ hybridization (FISH) techniques for detecting signals from restriction fragment length polymorphism (RFLP) clones. In the process, a single landmark plasmid, p18S5Shor, was constructed that identified and oriented all seven of the chromosome pairs. Plasmid p18S5Shor was used in all hybridizations. Fourteen cDNA probes selected from the linkage map for barley H. vulgare 'Steptoe' × H. vulgare 'Morex' (Kleinhofs et al. 1993) were mapped using an indirect tyramide signal amplification technique and assigned to a physical location on one or more chromosomes. The haploid barley genome is large and a complete physical map of the genome is not yet available; however, it was possible to integrate the linkage map and the physical locations of these cDNAs. An estimate of the ratio of base pairs to centimorgans was an average of 1.5 Mb/cM in the distal portions of the chromosome arms and 89 Mb/cM near the centromere. Furthermore, while it appears that the current linkage maps are well covered with markers along the length of each arm, the physical map showed that there are large areas of the genome that have yet to be mapped.Key words: Hordeum vulgare, barley, physical mapping, FISH, cDNA, genetics, linkage, chromosome, BACs.

scholarly journals Distribution of Bluetongue Virus in Tissues of Experimentally Infected Pregnant Dogs as Determined by In Situ Hybridization

1996 ◽  
Vol 33 (3) ◽  
pp. 337-340 ◽  
Author(s):  
C. C. Brown ◽  
J. C. Rhyan ◽  
M. J. Grubman ◽  
L. A. Wilbur
Keyword(s):  
In Situ Hybridization ◽  
Nucleic Acid ◽  
Bluetongue Virus ◽  
Mononuclear Cells ◽  
Nonstructural Protein ◽  
The Other ◽  
Post Inoculation ◽  
Viral Nucleic Acid ◽  
Nonstructural Protein 1

Six female dogs (four pregnant and two nonpregnant) were inoculated with bluetongue virus (BTV), serotype 11. Pregnant animals and one nonpregnant dog received 5.5-6.3 log10 of cell culture-adapted virus. The other nonpregnant dog received a modified live vaccine contaminated with bluetongue virus. The nonpregnant animals never became clinically ill and were euthanatized 35 days post-inoculation. Three of the four pregnant dogs aborted, and all four died or were euthanatized 5-10 days post-inoculation. The predominant pathologic feature in the adults was severe pulmonary edema. Various tissues from the bitches and fetuses were examined by in situ hybridization using a digoxigenin-labeled probe corresponding to the nonstructural protein-1 gene of BTV-17. By this technique, viral nucleic acid was detected predominantly in endothelial cells of lung of all four dogs, with lesser amounts in capillaries of uterus, spleen, and kidney in some of the dogs. In two adult dogs, bluetongue viral nucleic acid was detected in mononuclear cells of the periarteriolar lymphoid sheaths of spleen. There was minimal staining of capillaries in placentae in three of the five fetuses examined. There was no viral nucleic acid detected in any of the other fetal tissues.

scholarly journals Chlorine-Susceptible and Chlorine-Resistant Type 021N Bacteria Occurring in Bulking Activated Sludges

2001 ◽  
Vol 67 (11) ◽  
pp. 5303-5307 ◽  
Author(s):  
M. A. Séka ◽  
Y. Kalogo ◽  
F. Hammes ◽  
J. Kielemoes ◽  
W. Verstraete
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Morphological Features ◽  
The Other ◽  
Filamentous Bacteria ◽  
Staining Techniques ◽  
Effect Of Chlorine

ABSTRACT Two filamentous bacteria causing bulking in two activated sludges were examined. Investigations using morphological features, staining techniques, and fluorescent in situ hybridization identified both filaments as type 021N. However, an examination of the effect of chlorine on the sludges revealed a chlorine-susceptible type 021N in one sludge and a chlorine-resistant type 021N in the other.

Molecular cytogenetic characterization of an alloplasmic durum wheat line with a portion of chromosome 1D of Triticum aestivum carrying the scsae gene

Genome ◽  
2004 ◽  
Vol 47 (1) ◽  
pp. 206-214 ◽  
Author(s):  
Khwaja G Hossain ◽  
Oscar Riera-Lizarazu ◽  
Venugopal Kalavacharla ◽  
M Isabel Vales ◽  
Jamie L Rust ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
In Situ Hybridization ◽  
Durum Wheat ◽  
Triticum Turgidum ◽  
Aegilops Tauschii ◽  
Distal Region ◽  
Specific Gene ◽  
Homoeologous Recombination ◽  
Male Sterile

Triticum aestivum (2n = 6x = 42, AABBDD) with Triticum longissimum (2n = 2x = 14; S1S1) cytoplasm ((lo) cytoplasm) has normal fertility and plant vigor. However, the nucleus of durum wheat (Triticum turgidum (2n = 4x = 28, AABB)) is incompatible with the T. longissimum cytoplasm, producing non-viable progeny. This incompatibility is alleviated by scsae, a species cytoplasm-specific (scs) gene, on the long arm of chromosome 1D (1DL) of common wheat. The hemizygous (lo) durum scsae line is male sterile and is maintained by crossing to normal durum wheat. After pollination, the seeds produced are either plump and viable (with scsae) or shriveled and inviable (without scsae). Thus, the chromosome with scsae is inherited as a whole without recombination. The objectives of this study were to characterize the chromosome carrying scsae and to determine the process through which this gene was introgressed into the (lo) durum background. Molecular marker analysis with 27 probes and primers mapped to homoeologous group 1 and genomic in situ hybridization using differentially labeled total genomic DNA of durum wheat and Aegilops tauschii suggest the presence of a 1AL segment in place of the distal region of 1DL. Owing to the absence of any detectable duplications or deletions, homoeologous recombination is the most likely mechanism by which this introgression occurred.Key words: homoeologous recombination, in situ hybridization, nuclear-cytoplasmic interaction, species cytoplasm specific gene

Characterization by RFLP analysis and genomic in situ hybridization of a recombinant and a monosomic substitution plant derived from Hordeum vulgare L. × Hordeum bulbosum L. crosses

Genome ◽  
1997 ◽  
Vol 40 (2) ◽  
pp. 195-200 ◽  
Author(s):  
R. A. Pickering ◽  
A. M. Hill ◽  
R. G. Kynast
Keyword(s):  
In Situ Hybridization ◽  
Hordeum Vulgare ◽  
Rflp Analysis ◽  
Leaf Sheath ◽  
Genomic In Situ Hybridization ◽  
Barley Chromosome ◽  
Interspecific Crosses ◽  
Hordeum Bulbosum ◽  
Hordeum Vulgare L

Interspecific crosses in Hordeum have been made with the aim of transferring desirable traits, such as disease resistance, from a wild species, Hordeum bulbosum, into cultivated barley (Hordeum vulgare). Interspecific recombinants have previously been identified using several methods, but there are limitations with all the techniques. We improved our ability to characterize progeny from H. vulgare × H. bulbosum crosses by using genomic in situ hybridization (GISH). The plant material comprised a recombinant and a monosomic alien substitution plant derived from H. vulgare × H. bulbosum crosses. The recombinant possesses a pubescent leaf sheath conferred by a gene transferred from H. bulbosum into barley cultivar Golden Promise. The use of GISH on a plant homozygous for the pubescence gene confirmed the presence of H. bulbosum DNA located distally on two barley chromosomes and we mapped the introgression to barley chromosome 4HL using RFLP analysis. Furthermore, by means of an allelism test we found that the transferred gene for pubescence is allelic or closely linked to a gene for pubescence (Hs) located on barley chromosome 4HL. The presence of a single H. bulbosum chromosome in the monosomic substitution plant was confirmed by GISH. A distal introgression of H. bulbosum DNA was also observed on one barley chromosome, which was located on chromosome 3HL by RFLP analysis.Key words: Hordeum vulgare, Hordeum bulbosum, interspecific hybrid, gene introgression, genomic in situ hybridization.

Identification of all chromosome arms and their involvement in meiotic homoeologous associations at metaphase I in 2 Hordeum vulgare L. × Hordeum bulbosum L. hybrids

Genome ◽  
2006 ◽  
Vol 49 (1) ◽  
pp. 73-78 ◽  
Author(s):  
R Pickering ◽  
S Klatte ◽  
R C Butler
Keyword(s):  
In Situ Hybridization ◽  
Hordeum Vulgare ◽  
Fluorescence In Situ Hybridization ◽  
Interspecific Hybrids ◽  
Association Studies ◽  
Hordeum Bulbosum ◽  
Hordeum Vulgare L ◽  
Chromosome Addition ◽  
Chromosome Associations

We have identified all Hordeum bulbosum chromosomes in 2 diploid Hordeum vulgare × Hordeum bulbosum hybrids using suitable probes and fluorescence in situ hybridization. Using the parental idiograms allowed us to carry out a full analysis of chromosome associations among all chromosome arms in the hybrids. Association frequencies were generally lower for the short arms than for the long arms. There were also significant differences among the chromosome arms in association frequencies, partly correlated with the absolute length of the chromosome arm, as well as with the frequency of recombinant lines, which were recovered from partially fertile interspecific hybrids. The H. bulbosum idiogram will be useful for further chromosome association studies and will enable the identification of H. bulbosum chromosomes involved in chromosome addition or substitution lines.Key words: Hordeum vulgare, Hordeum bulbosum, interspecific hybrids, chromosome associations, meiosis, fluorescence in situ hybridization.

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