An SSR-based genetic linkage map of the model grass Brachypodium distachyon

Genome ◽  
2010 ◽  
Vol 53 (1) ◽  
pp. 1-13 ◽  
Author(s):  
David F. Garvin ◽  
Neil McKenzie ◽  
John P. Vogel ◽  
Todd C. Mockler ◽  
Zachary J. Blankenheim ◽  
...  
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Expressed Sequence Tag ◽  
Brachypodium Distachyon ◽  
Molecular Genetic ◽  
Rice Genome ◽  
Artificial Chromosome ◽  
Grass Species ◽  
Linkage Groups

The grass species Brachypodium distachyon (hereafter, Brachypodium) has been adopted as a model system for grasses. Here, we describe the development of a genetic linkage map of Brachypodium. The genetic linkage map was developed with an F2 population from a cross between the diploid Brachypodium lines Bd3-1 and Bd21. The map was populated with polymorphic simple sequence repeat (SSR) markers from Brachypodium expressed sequence tag (EST) and bacterial artificial chromosome (BAC) end sequences and conserved orthologous sequence (COS) markers from other grass species. The map is 1386 cM in length and consists of 139 marker loci distributed across 20 linkage groups. Five of the linkage groups exceed 100 cM in length, with the largest being 231 cM long. Assessment of colinearity between the Brachypodium linkage map and the rice genome sequence revealed significant regions of macrosynteny between the two genomes, as well as rearrangements similar to those reported in other grass comparative structural genomics studies. The Brachypodium genetic linkage map described here will serve as a new tool to pursue a range of molecular genetic analyses and other applications in this new model plant system.

A molecular genetic linkage map identifying the St and H subgenomes of Elymus (Poaceae: Triticeae) wheatgrass

Genome ◽  
2011 ◽  
Vol 54 (10) ◽  
pp. 819-828 ◽  
Author(s):  
Ivan W. Mott ◽  
Steven R. Larson ◽  
Thomas A. Jones ◽  
Joseph G. Robins ◽  
Kevin B. Jensen ◽  
...  
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Molecular Genetic ◽  
Perennial Grass ◽  
Rice Genome ◽  
Backcross Progeny ◽  
Linkage Groups ◽  
Sts Markers ◽  
Snake River Wheatgrass

Elymus L. is the largest and most complex genus in the Triticeae tribe of grasses with approximately 150 polyploid perennial species occurring worldwide. We report here the first genetic linkage map for Elymus. Backcross mapping populations were created by crossing caespitose Elymus wawawaiensis (EW) (Snake River wheatgrass) and rhizomatous Elymus lanceolatus (EL) (thickspike wheatgrass) to produce F1 interspecific hybrids that were then backcrossed to the same EL male to generate progeny with segregating phenotypes. EW and EL are both allotetraploid species (n = 14) containing the St (Pseudoroegneria) and H (Hordeum) genomes. A total of 387 backcross progeny from four populations were genotyped using 399 AFLP and 116 EST-based SSR and STS markers. The resulting consensus map was 2574 cM in length apportioned among the expected number of 14 linkage groups. EST-based SSR and STS markers with homology to rice genome sequences were used to identify Elymus linkage groups homoeologous to chromosomes 1–7 of wheat. The frequency of St-derived genome markers on each linkage group was used to assign genome designations to all linkage groups, resulting in the identification of the seven St and seven H linkage groups of Elymus. This map also confirms the alloploidy and disomic chromosome pairing and segregation of Elymus and will be useful in identifying QTLs controlling perennial grass traits in this genus.

Development of a deletion and genetic linkage map for the 5A and 5B chromosomes of wheat (Triticum aestivum)

Genome ◽  
2012 ◽  
Vol 55 (6) ◽  
pp. 417-427 ◽  
Author(s):  
A. Gadaleta ◽  
A. Giancaspro ◽  
S.L. Giove ◽  
S. Zacheo ◽  
O. Incerti ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Linkage Map ◽  
Chinese Spring ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Oryza Sativa L ◽  
Brachypodium Distachyon ◽  
Triticum Aestivum L ◽  
Linkage Groups ◽  
Chromosome 5A

The aims of the present study were to provide deletion maps for wheat ( Triticum aestivum L.) chromosomes 5A and 5B and a detailed genetic map of chromosome 5A enriched with popular microsatellite markers, which could be compared with other existing maps and useful for mapping major genes and quantitative traits loci (QTL). Physical mapping of 165 gSSR and EST–SSR markers was conducted by amplifying each primer pair on Chinese Spring, aneuploid lines, and deletion lines for the homoeologous group 5 chromosomes. A recombinant inbred line (RIL) mapping population that is recombinant for only chromosome 5A was obtained by crossing the wheat cultivar Chinese Spring and the disomic substitution line Chinese Spring-5A dicoccoides and was used to develop a genetic linkage map of chromosome 5A. A total of 67 markers were found polymorphic between the parental lines and were mapped in the RIL population. Sixty-three loci and the Q gene were clustered in three linkage groups ordered at a minimum LOD score of 5, while four loci remained unlinked. The whole genetic 5A chromosome map covered 420.2 cM, distributed among three linkage groups of 189.3, 35.4, and 195.5 cM. The EST sequences located on chromosomes 5A and 5B were used for comparative analysis against Brachypodium distachyon (L.) P. Beauv. and rice ( Oryza sativa L.) genomes to resolve orthologous relationships among the genomes of wheat and the two model species.

Constructing a genetic linkage map and mapping quantitative trait loci for skeletal traits in Japanese flounder

Biologia ◽  
2013 ◽  
Vol 68 (6) ◽  
Author(s):  
Yi Liu ◽  
Yongxin Liu ◽  
Yingjie Liu ◽  
Xiaoyan Zhang ◽  
Fei Si ◽  
...  
Keyword(s):  
Linkage Map ◽  
Quantitative Trait ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Expressed Sequence Tag ◽  
Average Distance ◽  
Doubled Haploids ◽  
Japanese Flounder ◽  
Type Ii ◽  
Linkage Groups

AbstractA genetic linkage map of Japanese flounder was constructed using 165 doubled haploids (DHs) derived from a single female. A total of 574 genomic microsatellites (type II SSRs) and expressed sequence tag (EST)-derived markers (EST-SSRs) were mapped to 24 linkage groups. The length of linkage map was estimated as 1270.9 centiMorgans (cM), with an average distance between markers of 2.2 cM. The EST-SSRs were used together with type II SSR markers to construct the Japanese flounder genetic linkage map which will facilitate identify quantitative trait locus (QTL) controlling important economic traits in Japanese flounder. Thus, twelve skeletal traits at 2 years of age were measured for all DHs. Forty-one QTLs were detected on 14 linkage groups and totally account for a small proportion of phenotypic variation (4.5 to 17.3%). Most of QTLs detected distribute on linkage groups 5 (9 QTLs), 8 (9 QTLs), 9 (5 QTLs) and 20 (4 QTLs), in which, some QTLs perform the pleiotropy.

Towards a Saturated Molecular Genetic Linkage Map for Cultivated Sunflower

Crop Science ◽  
2003 ◽  
Vol 43 (1) ◽  
pp. 367 ◽  
Author(s):  
Ju-Kyung Yu ◽  
Shunxue Tang ◽  
Mary B. Slabaugh ◽  
Adam Heesacker ◽  
Glenn Cole ◽  
...  
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Molecular Genetic

Development of a molecular genetic linkage map for Colletotrichum lindemuthianum and segregation analysis of two avirulence genes

2006 ◽  
Vol 51 (2) ◽  
pp. 109-121 ◽  
Author(s):  
Francisco Luna-Martínez ◽  
Raúl Rodríguez-Guerra ◽  
Mayra Victoria-Campos ◽  
June Simpson
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Segregation Analysis ◽  
Genetic Linkage Map ◽  
Molecular Genetic ◽  
Colletotrichum Lindemuthianum ◽  
Avirulence Genes

scholarly journals Genetic Linkage Map of the Edible BasidiomycetePleurotus ostreatus

2000 ◽  
Vol 66 (12) ◽  
pp. 5290-5300 ◽  
Author(s):  
Luis M. Larraya ◽  
G�mer P�rez ◽  
Enrique Ritter ◽  
Antonio G. Pisabarro ◽  
Lucı́a Ramı́rez
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Gene Sequence ◽  
Genetic Linkage Map ◽  
Fragment Length Polymorphism ◽  
Individual Cell ◽  
Random Amplified Polymorphic Dna ◽  
Rrna Gene ◽  
Linkage Groups ◽  
Rrna Gene Sequence

ABSTRACT We have constructed a genetic linkage map of the edible basidiomycete Pleurotus ostreatus (var. Florida). The map is based on the segregation of 178 random amplified polymorphic DNA and 23 restriction fragment length polymorphism markers; four hydrophobin, two laccase, and two manganese peroxidase genes; both mating type loci; one isozyme locus (est1); the rRNA gene sequence; and a repetitive DNA sequence in a population of 80 sibling monokaryons. The map identifies 11 linkage groups corresponding to the chromosomes ofP. ostreatus, and it has a total length of 1,000.7 centimorgans (cM) with an average of 35.1 kbp/cM. The map shows a high correlation (0.76) between physical and genetic chromosome sizes. The number of crossovers observed per chromosome per individual cell is 0.89. This map covers nearly the whole genome of P. ostreatus.

CONSTRUCTION OF A MOLECULAR GENETIC LINKAGE MAP FOR LONGAN BASED ON RAPD, ISSR, SRAP AND AFLP MARKERS

2010 ◽  
pp. 141-148 ◽  
Author(s):  
Y.S. Guo ◽  
C.J. Liu ◽  
Y.H. Zhao ◽  
J.H. Xie ◽  
P.R. Ren ◽  
...  
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Molecular Genetic ◽  
Aflp Markers

A molecular genetic linkage map of mouse chromosome 2

Genomics ◽  
1990 ◽  
Vol 6 (3) ◽  
pp. 491-504 ◽  
Author(s):  
Linda D. Siracusa ◽  
Colleen M. Silan ◽  
Monica J. Justice ◽  
John A. Mercer ◽  
Asne R. Bauskin ◽  
...  
Keyword(s):  
Linkage Map ◽  
Mouse Chromosome ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Molecular Genetic ◽  
Chromosome 2 ◽  
Mouse Chromosome 2
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