125 years of experimental heat shock research: historical roots of a discipline

Genome ◽  
1989 ◽  
Vol 31 (2) ◽  
pp. 668-670 ◽  
Author(s):  
Lutz Nover
Keyword(s):  
Heat Shock ◽  
Skin Cancer ◽  
Heat Shock Response ◽  
Cell Biology ◽  
Spontaneous Regression ◽  
Local Inflammation ◽  
Major Field ◽  
Experimental Heat ◽  
Shock Response ◽  
History Of

The history of experimental heat shock research over the last 125 years is briefly outlined. Starting with reports on the upper temperature limits of plant survival (1864) and on the spontaneous regression of skin cancer after a severe local inflammation (1866), studies on the heat shock response today are a major field of modern cell biology and include all types of prokaryotic and eukaryotic organisms.Key words: history, heat shock research.

Dimethyl-sulfoxide is a Suitable Solvent for Fluorescent Microscopy Detection of Medium and Strong Heat Shock Inductors Using Transgenic Zebrafish

2018 ◽  
Vol 69 (2) ◽  
pp. 337-340
Author(s):  
Vlad Preluca ◽  
Bogdan Horatiu Serb ◽  
Sanda Marchian ◽  
Diter Atasie ◽  
Mihaela Cernusca Mitariu ◽  
...  
Keyword(s):  
Heat Shock ◽  
Dimethyl Sulfoxide ◽  
Heat Shock Response ◽  
Protein Misfolding ◽  
Fluorescent Microscopy ◽  
Screening Tests ◽  
Transgenic Zebrafish ◽  
Fluorescent Intensity ◽  
Shock Response ◽  
Heat Shock Induction

Heat shock inductors have potential as treatment for degenerative and protein misfolding diseases. Dimethyl-sulfoxide is widely used as a solvent in pharmacological screening tests and has been shown to have heat shock induction effects. Transgenic Tg (hsp70l:EGFP-HRAS_G12V)io3(AB) zebrafish larvae were exposed for 24 hours to dimethyl-sulfoxide in concentratios of 0.1-2%, and to moderate heat shock inductors pentoxifylline and tacrolimus. Positive controls were exposed to 35, 38 and 40�C for 20 min, and incubated for 24 h at 28�C. Heat shock response was measured by fluorescence microscopy and signal intensity quantification in FIJI. Dimethyl-sulfoxide caused a dose-dependant increase in fluorescent intensity, but significantly lower compared with exposure to 38 and 40�C. Pentoxifylline and tacrolimus induced a significantly higher increase in fluorescence compared with 0.5% dimethyl-sulfoxide. Thus, although dimethyl-sulfoxide has independent heat shock induction effects, concentrations of up to 0.5% are suitable for heat shock response screening tests.

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