METHODS OF PROTEIN EXTRACTION FROM NEUROSPORA CRASSA

1964 ◽  
Vol 10 (1) ◽  
pp. 29-35 ◽  
Author(s):  
G. J. Stine ◽  
W. N. Strickland ◽  
R. W. Barratt
Keyword(s):  
Glutamic Acid ◽  
Neurospora Crassa ◽  
Total Protein ◽  
Extraction Method ◽  
Protein Extraction ◽  
Specific Activity ◽  
Dry Weight ◽  
Acid Dehydrogenase ◽  
Total Enzyme

Nine methods for disrupting the mycelium of Neurospora crassa have been compared. Protein percentages are calculated per gram dry weight of mycelium. A TPN-specific glutamic acid dehydrogenase was extracted and the efficiency of each extraction method is given as total enzyme extracted and specific activity. In terms of total protein, total enzyme, and practicality of the method, the Hughes Press, the French Press and the Raper–Hyatt Press were found to be the most efficient. The advantages and limitations of each method are considered.

A Method Suitable for Total Protein Extraction from Wood Forming Tissue of Pinus koraiensis

2011 ◽  
Vol 179-180 ◽  
pp. 1199-1202
Author(s):  
Jiang Tao Shi ◽  
Jian Li
Keyword(s):  
Total Protein ◽  
Extraction Method ◽  
Protein Extraction ◽  
Pinus Koraiensis ◽  
Total Proteins ◽  
Sds Page ◽  
Quality Product

Total protein extraction method should be adjusted for get higher quality product for special material. We optimized the protocols and extracted total proteins from P. koraiensis adopt TCA-acetone, TCA-acetone contains β-mercaptoethanol, methanol and protein extraction kit respectively. Then we compared extracts from these methods using SDS-PAGE. The results show that there are no obvious differences between TCA, TCA-2ME and Methanol, which all have better bands. In contrast, extracts from protein extraction kit had ambiguous bands. We also found that it should take about eight hours at least for precipitate of mixtures of samples and extraction solutions. We think optimized TCA-acetone is an idea method for protein extraction from P. koraiensis.

scholarly journals A further genetic variety of glutamic acid dehydrogenase in Neurospora crassa

1960 ◽  
Vol 77 (1) ◽  
pp. 96-105 ◽  
Author(s):  
J. R. S. Fincham ◽  
P. A. Bond
Keyword(s):  
Glutamic Acid ◽  
Neurospora Crassa ◽  
Acid Dehydrogenase

Verification of Protein Extraction Method by Magneto-Ferrite Treatment with EDS Analysis

2020 ◽  
Vol 140 (2) ◽  
pp. 128-129
Author(s):  
Suguru Kotani ◽  
Masaya Endo ◽  
Mahmudul Kabir ◽  
Kazutaka Mitobe
Keyword(s):  
Extraction Method ◽  
Protein Extraction ◽  
Eds Analysis

Synthesis and properties of [2-[3,5-3H2]-tyrosine,4-glutamic acid]deamino-1-carba-oxytocin

1984 ◽  
Vol 49 (8) ◽  
pp. 1921-1926 ◽  
Author(s):  
Michal Lebl ◽  
Tomislav Barth ◽  
Denis J. Crankshaw ◽  
Bohuslav Černý ◽  
Edwin E. Daniel ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Glutamic Acid ◽  
Title Compound ◽  
Specific Activity ◽  
Reversed Phase ◽  
Reversed Phase Liquid Chromatography ◽  
Phase Liquid

The title compound (specific activity 11.1-32.7 Ci (0.41-1.22 TBq)/mmol) was prepared by iodination and subsequent catalytic replacement of iodine by tritium. The analogue which was unstable in the form of a lyophilizate was purified by reversed phase liquid chromatography. Using the N,N'-dicyclohexylcarbodiimide method, the pure analogue was converted into N-hydroxybenzotriazolyl ester, an irreversible oxytocin inhibitor. However, attempts to label specifically the uterotonic receptor, present in the enriched rat myometrium fraction, were hithero unsuccessful

Effect of cations on the activity of L-glutamic acid dehydrogenase

Life Sciences ◽  
1963 ◽  
Vol 2 (11) ◽  
pp. 834-839 ◽  
Author(s):  
E. Schoffeniels ◽  
R. Gilles
Keyword(s):  
Glutamic Acid ◽  
Acid Dehydrogenase

scholarly journals An improved protein extraction method applied to cotton leaves is compatible with 2-DE and LC-MS

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Xiang Jin ◽  
Liping Zhu ◽  
Chengcheng Tao ◽  
Quanliang Xie ◽  
Xinyang Xu ◽  
...  
Keyword(s):  
Extraction Method ◽  
Protein Extraction

scholarly journals Molecular Weight of Chicken Liver Glutamic Acid Dehydrogenase in Relation to the Activating Effect of Methylmercuric Bromide

1963 ◽  
Vol 238 (1) ◽  
pp. PC481-PC482
Author(s):  
Kenneth S. Rogers ◽  
Paul J. Geiger ◽  
Thomas E. Thompson ◽  
Leslie Hellerman
Keyword(s):  
Molecular Weight ◽  
Glutamic Acid ◽  
Chicken Liver ◽  
Acid Dehydrogenase
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