Deoxyribonucleic acid base composition of isolates of Rhizobium japonicum

1969 ◽  
Vol 15 (5) ◽  
pp. 490-493 ◽  
Author(s):  
Gerald H. Elkan
Keyword(s):  
Statistical Analysis ◽  
Base Composition ◽  
Deoxyribonucleic Acid ◽  
Genetic Relatedness ◽  
The Other ◽  
Rhizobium Japonicum ◽  
Acid Base ◽  
Dna Base Composition ◽  
Dna Base ◽  
Mole Percentage

The DNA base composition (expressed as mean molar percentages of guanine and cytosine) of 25 strains of Rhizobium japonicum was determined to study the genetic relatedness of these strains. The mole percentage GC ranged from 61.47 to 64.09. Statistical analysis of the DNA base ratios of the 25 isolates revealed that 16 of these differed significantly in GC ratios from some of the other strains.

scholarly journals II. Charakterisierung der schnellmarkierten RNS

1968 ◽  
Vol 23 (9) ◽  
pp. 1198-1208 ◽  
Author(s):  
R. Wollgiehn ◽  
M. Ruess
Keyword(s):  
Base Composition ◽  
Actinomycin D ◽  
Gc Content ◽  
The Other ◽  
Nucleic Acid Metabolism ◽  
Dna Base Composition ◽  
Dna Base ◽  
Molecular Fraction ◽  
Young Leaves ◽  
Short Time

The nucleic acid metabolism of young leaves (Nicotiana rustica, Pisum sativum) was investigated by 32P-labelling in short-time experiments. The nucleic acids were extracted quantitatively with buffer, containing SDS, and fractionated on the MAK column. Several rapidly labelled RNA fractions were found in the region of sRNS, DNA and rRNA. We have characterized two rapidly labelled fractions more detailed. The first one is a high molecular AU-RNA, which is eluted from the MAK column after the rRNA. The second one is a low molecular fraction in the region of the sRNA.The AU-RNA has the following properties: 1) It is rapidly labelled in pulse experiments. 2) the halfe-life of the AU-RNA in chase experiments is 4 hours at its maximum, showing faster degradation compared to the other fractions. 3) The 32P-incorporation is quantitatively inhibited by actinomycin D. 4) The base composition resembles DNA base composition (high AU and low GC content) . 5) AU-RNS appears as a heterogeneous fraction in the sucrose gradient, and sediments similar to rRNA. 6) AU-RNA occurs in all particle fractions of the homogenates.The low molecular, rapidly labelled RNA shows the following properties: 1) The radioactive peak does not correspond with 4 S or 5 S RNA peaks. 2) This RNA is metabolically stable, the radioactivity decreases very slowly in chase-experiments. 3) The 32P-incorporation is less inhibited by actinomycin D than the other RNA fractions. 4) The base composition is not similar to DNA base composition. A high content of labelled CTP in this RNA fraction may indicate the rapid 32P-incorporation by exchange of the terminal CpCpA group of tRNA.The possible messenger function of the AU-RNA is discussed.

DNA BASE COMPOSITION OF CYTOPHAGA MARINOFLAVA N. SP. DETERMINED BY BUOYANT DENSITY MEASUREMENTS IN CESIUM CHLORIDE

1966 ◽  
Vol 12 (6) ◽  
pp. 1099-1103 ◽  
Author(s):  
R. R. Colwell ◽  
R. V. Citarella ◽  
P. K. Chen
Keyword(s):  
Nucleic Acid ◽  
Base Composition ◽  
Marine Bacterium ◽  
Deoxyribonucleic Acid ◽  
Buoyant Density ◽  
Cesium Chloride ◽  
Dna Base Composition ◽  
Density Measurements ◽  
Dna Base ◽  
Relationship Of

A marine bacterium, NCMB 397, host strain for bacteriophages NCMB 384 and 385, has been subjected to taxonoinic analysis. Overall base composition of the highly purified deoxyribonucleic acid was determined and found to be 37 moles % guanine + cytosine. The phenetic and nucleic acid data suggest significant relationship of this strain and members of the genus Cytophaga. A description of Cytophaga marinoflava n. sp. is presented.

Deoxyribonucleic acid relationships among members of the genus Aeromonas

1979 ◽  
Vol 25 (5) ◽  
pp. 579-586 ◽  
Author(s):  
Jan I. MacInnes ◽  
Trevor J. Trust ◽  
Jorge H. Crosa
Keyword(s):  
Aeromonas Hydrophila ◽  
Base Composition ◽  
Deoxyribonucleic Acid ◽  
Single Species ◽  
Aeromonas Salmonicida ◽  
Dna Duplexes ◽  
Dna Base Composition ◽  
Homology Groups ◽  
Dna Base ◽  
Significant Divergence

Polynucleotide sequences among 24 motile and 11 non-motile aeromonads were studied by analysis of deoxyribonucleic acid – deoxyribonucleic acid (DNA–DNA) duplexes with endonuclease S1. In addition, DNA base composition (mole % guanine and cytosine (G + C) and relative genome sizes were determined for selected strains. Large variations in genome size were found and % GC ranged from 57.1 to 62.9%. On the basis of the strains examined, the Genus Aeromonas consists of two genotypically legitimate groups: a diverse group of motile aeromonads, and the genetically more homogeneous non-motile aeromonads, comprising the species Aeromonas salmonicida. Internal homology groups could not be demonstrated within the motile aeromonads, and significant divergence in related sequences was indicated. This diverse motile group forms the single species Aeromonas hydrophila.

Mutants with altered DNA base composition inBacterium paracoli — the evidence from a marker

1971 ◽  
Vol 11 (2) ◽  
pp. 91-95 ◽  
Author(s):  
G. F. Gause ◽  
A. V. Laiko ◽  
M. V. Bibikova ◽  
L. I. Kusovkova ◽  
T. I. Selesneva ◽  
...  
Keyword(s):  
Base Composition ◽  
Dna Base Composition ◽  
Dna Base

DNA base composition heterogeneity in some agarophytes (Gracilariales, Rhodophyta) from Mexico and the Philippines

1996 ◽  
Vol 8 (3) ◽  
pp. 229-237 ◽  
Author(s):  
Donald F. Kapraun ◽  
Juan Lopez-Bautista ◽  
Kimon T. Bird
Keyword(s):  
Base Composition ◽  
The Philippines ◽  
Dna Base Composition ◽  
Dna Base
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