Soil bacteria: numerical analysis of electrophoretic protein patterns developed in acrylamide gels

1970 ◽  
Vol 16 (3) ◽  
pp. 202-205 ◽  
Author(s):  
J. W. Rouatt ◽  
G. W. Skyring ◽  
Vesna Purkayastha ◽  
C. Quadling
Keyword(s):  
Numerical Analysis ◽  
Numerical Method ◽  
Soil Bacteria ◽  
Protein Band ◽  
Soluble Proteins ◽  
Protein Patterns ◽  
Electrophoretic Band ◽  
Crude Extracts ◽  
Unit Character ◽  
Microbial Taxonomy

The protein band patterns of 47 soil bacteria, all thought to be of the arthrobacter kind, were analyzed by a simple numerical method in which each observed Et value was treated as a unit character. The results were not satisfactory. In a second analysis only major bands were taken into account and small variations in the Et values of the 24 chosen bands were disregarded. This subjective editing was required to satisfy a minimum criterion that replicates be recognized as such by the analysis. It was concluded that without a means of estimating the variation in Et value for each band and of identifying homologous bands, electrophoretic band patterns of crude extracts of soluble proteins are likely to be of limited rather than general use in microbial taxonomy.

Adsorption on heterogeneous surfaces: Numerical analysis of models for the Dubinin-Radushkevich equation

1981 ◽  
Vol 46 (8) ◽  
pp. 1709-1721 ◽  
Author(s):  
Miloš Smutek ◽  
Arnošt Zukal
Keyword(s):  
Integral Equation ◽  
Numerical Analysis ◽  
Numerical Method ◽  
Adsorption Behaviour ◽  
Heterogeneous Surfaces ◽  
Strong Energy ◽  
Adsorption On Heterogeneous Surfaces ◽  
Heterogeneity Parameter ◽  
Adsorption Energies ◽  
Mobile Adsorption

A numerical method, based on the integral equation of the adsorption on energy heterogeneous surfaces, is suggested for the evaluation of overall isotherm. It is shown that for the distribution of adsorption energies given by Eq. (1.11) and different models of the adsorption behaviour, the overall isotherms obey approximately the Dubinin-Radushkevich equation. The strong energy heterogeneity smears effectively the differences between the localized and mobile adsorption and leads to the same character of the overall isotherm with only a slightly changed heterogeneity parameter.

scholarly journals Growth optimization and identification of an ω-transaminase by a novel native PAGE activity staining method in a Bacillus sp. strain BaH isolated from Iranian soil

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Najme Gord Noshahri ◽  
Jamshid Fooladi ◽  
Ulrike Engel ◽  
Delphine Muller ◽  
Michaela Kugel ◽  
...  
Keyword(s):  
Wild Type Strain ◽  
Colorimetric Assay ◽  
Protein Band ◽  
Wild Type ◽  
Direct Identification ◽  
Native Page ◽  
Crude Extracts ◽  
Amino Donor ◽  
Bioreactor System ◽  
Type Strains

Abstractω-Transaminases’ (ω-TAs) importance for synthesizing chiral amines led to the development of different methods to quickly identify and characterize new sources of these enzymes. Here we describe the optimization of growth and induction of such an enzyme in a wild type strain of Bacillus sp. strain BaH (IBRC-M 11337) isolated from Iranian soil in shaking flasks by the response surface methodology (RSM). Optimum conditions were set in a multiplexed bench-top bioreactor system (Sixfors). ω-TA activity of obtained biomass was checked by an innovative efficient colorimetric assay for localizing ω-TAs in crude extracts on acrylamide gel by using ortho-xylylenediamine (OXD) as amino donor. The application of the established OXD assay is thereby expanded from high-throughput activity screenings and colony-based screenings of heterologously expressed mutants to a direct identification of ω-TAs in wild-type strains: This assay can be used to detect the protein band of the respective enzyme in crude extracts of novel isolates by visual inspection of native PAGEs without any upstream protein purification, thus enabling subsequent further investigations of a newly discovered enzyme directly from the crude extract.

scholarly journals Numerical analysis of stress distribution generated in spherical polyethylene inserts by knee joint endoprotheses’ sleds

2019 ◽  
Vol 21 (2) ◽  
pp. 1-5
Author(s):  
Marcin Nabrdalik ◽  
Michał Sobociński
Keyword(s):  
Numerical Analysis ◽  
Finite Elements ◽  
Stress Distribution ◽  
Knee Joint ◽  
Titanium Alloys ◽  
Numerical Method ◽  
Ti6al4v Alloy ◽  
Finite Elements Method ◽  
Weak Points ◽  
Metal Materials

Abstract The paper presents analysis of stress distribution in the friction node of knee joint endoprosthesis where sleds are made of various titanium alloys and CoCrMo cooperate with spherical polyethylene inserts. Currently used titanium alloys consists of Nb, Ta, Zr or Mo and with lesser value of Young’s modulus than Ti6Al4V alloy, or steel CoCrMo, which significantly varies from other metal materials. The obtained results make it possible to indicate the “weak points” of the accepted solution, and thus counteract the subsequent effects resulting from premature wear of endoprosthesis elements. The analysis was conducted with numerical method of ADINA System 8.6. The Finite Elements Method allowed to compute and present stress distribution quickly in all elements of the model.

Coalescence Characteristics of Fusible Particles in Solderable Isotropic Conductive Adhesives (ICAs)

2008 ◽  
Vol 580-582 ◽  
pp. 213-216
Author(s):  
Seong Hyuk Lee ◽  
Jung Hee Lee ◽  
Jin Woon Lee ◽  
Jong Min Kim
Keyword(s):  
Numerical Analysis ◽  
Numerical Method ◽  
Volume Fraction ◽  
Conductive Adhesive ◽  
Conductive Adhesives ◽  
Joining Technology ◽  
Isotropic Conductive Adhesives

A new hybrid soldering and conductive adhesive joining technology using a fusible lowmelting- point alloy (LMPA) have been developed. A numerical method for numerical analysis of fusible particles behavior is proposed to investigate coalescence characteristics of fusible particles in solderable isotropic conductive adhesives (ICAs). For finding out suitable conditions to obtain reliable conduction paths, the present study examines the influence of process-related parameters such as volume fraction and viscosity on coalescence characteristics of fusible particles.

Numerical Analysis of Impinging Air Flow and Heat Transfer in Plate-Fin Type Heat Sinks

1999 ◽  
Vol 123 (3) ◽  
pp. 315-318 ◽  
Author(s):  
Keiji Sasao ◽  
Mitsuru Honma ◽  
Atsuo Nishihara ◽  
Takayuki Atarashi
Keyword(s):  
Heat Transfer ◽  
Thermal Conductivity ◽  
Numerical Analysis ◽  
Numerical Method ◽  
Heat Sink ◽  
Flow Resistance ◽  
Air Flow ◽  
Heat Sinks ◽  
Flow And Heat Transfer ◽  
Conventional Methods

A numerical method for simulating impinging air flow and heat transfer in plate-fin type heat sinks has been developed. In this method, all the fins of an individual heat sink and the air between them are replaced with a single, uniform element having an appropriate flow resistance and thermal conductivity. With this element, fine calculation meshes adapted to the shape of the actual heat sink are not needed, so the size of the calculation mesh is much smaller than that of conventional methods.

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