Changes in cutaneous flora after wet occlusion

1975 ◽  
Vol 21 (4) ◽  
pp. 496-500 ◽  
Author(s):  
David J. Bibel ◽  
Joseph R. LeBrun
Keyword(s):  
Enterobacter Aerogenes ◽  
Plating Technique ◽  
Replica Plating ◽  
Subgroup Ii ◽  
Colony Forming Units ◽  
Recovery Response ◽  
Aerobic Flora ◽  
Almost All

Aerobic flora from wet-occluded forearms of six volunteers was sampled the day before treatment, on the 3rd day when dressings were removed, and daily, when possible, for 8 days thereafter. Erythema was not present. All bacterial colonies appearing on appropriate dilution plates were identified with the aid of a replica-plating technique. Flora of each individual increased to over 104 colony-forming units/cm2 as a result of wet-occlusion, but counts rapidly fell by about 102 units once dressings were removed. Although similar types of bacteria were found on all subjects, the composition of each individual's flora during the recovery response appeared to be unique. Enterobacteriaceae were found on half the subjects with Enterobacter aerogenes being the most successful colonizer. Besides the expected presence of Baird-Parker Staphylococcus subgroup II, high numbers of subgroup IV and some colonies of subgroup III were also observed. Almost all cutaneous diphtheroids were lipophilic and lipolytic.

REPLICA PLATING TECHNIQUE FOR STUDYING MICROBIAL INTERACTIONS IN SOIL

1965 ◽  
Vol 11 (4) ◽  
pp. 629-636 ◽  
Author(s):  
G. Stotzky
Keyword(s):  
Nutritional Composition ◽  
Mixed Population ◽  
Microbial Interactions ◽  
Individual Species ◽  
Selective Inhibitors ◽  
Subsequent Growth ◽  
Sterile Soil ◽  
Plating Technique ◽  
Replica Plating ◽  
Plating Method

A replica plating method was developed to study ecology of microorganisms in soil. Precise placement of inocula and amendments at desired loci in sterile soil contained in petri plates were accomplished with a template. Subsequent growth and distribution of individual species, even when part of a mixed population, was measured by periodic transfer with an easily constructed replicator to agar plates of differing nutritional composition or containing selective inhibitors. The method is rapid and reproducible, and permits the study of many variables and interactions in a single soil plate; it can also be used with non-sterile soil and other suitable microbial habitats.

A Photographic Method for the Detection of Rare Bacterial Mutants in the Replica Plating Technique

Nature ◽  
1963 ◽  
Vol 199 (4889) ◽  
pp. 198-199 ◽  
Author(s):  
J. H. VAN DE POL ◽  
C. M. A. VENDRIG ◽  
G. A. VAN ARKEL
Keyword(s):  
Photographic Method ◽  
Plating Technique ◽  
Replica Plating ◽  
Bacterial Mutants

scholarly journals A rapid and efficient replica plating technique

1970 ◽  
Vol 15 (3) ◽  
pp. 335-338 ◽  
Author(s):  
Thomas H. Wood ◽  
Suresh K. Mahajan
Keyword(s):  
Plating Technique ◽  
Replica Plating ◽  
Operator Error ◽  
New Type

SUMMARYA new type of apparatus for replica plating is described, which reduces operator error and fatigue. Several replica plates can be made immediately, thus eliminating the need for a master plate in many cases.

scholarly journals Alternative Replica Plating Technique

1977 ◽  
Vol 34 (2) ◽  
pp. 225-227 ◽  
Author(s):  
E. Börje Lindström
Keyword(s):  
Plating Technique ◽  
Replica Plating

scholarly journals Killing efficacy of a new hypothermic corneal storage medium against the micro-organisms frequently found in human donor cornea intended for transplantation

2021 ◽  
Vol 6 (1) ◽  
pp. e000833
Author(s):  
Laura Giurgola ◽  
Claudio Gatto ◽  
Claudia Honisch ◽  
Orietta Rossi ◽  
Eugenio Ragazzi ◽  
...  
Keyword(s):  
Microbial Count ◽  
Human Donor ◽  
Aspergillus Brasiliensis ◽  
Colony Forming Units ◽  
Corneal Storage ◽  
Hypothermic Storage ◽  
Almost All ◽  
Micro Organisms ◽  
Donor Corneas

ObjectiveTo study the in vitro killing efficacy of Kerasave (AL.CHI.MI.A Srl), a medium provided with amphotericin B tablet for hypothermic storage of human donor corneas, against relevant contaminants associated with postkeratoplasty infections.Methods and AnalysisThe antimicrobial activity of Kerasave was determined after 0, 3 and 14 days of incubation at 2°C–8°C, inoculating Kerasave and the control medium with 105–106 colony forming units (CFU) of Candida albicans (CA), Fusarium solani (FS), Aspergillus brasiliensis (AB), Staphylococcus aureus (SA), Enterococcus faecalis (EF), Bacillus subtilis spizizenii (BS), Pseudomonas aeruginosa (PA), Enterobacter cloacae (EC) and Klebsiella pneumoniae (KP). Log10 reductions at different time intervals were determined by assessing the number of viable CFU using the serial dilution plating technique.ResultsAfter 3 days, Kerasave induced the highest log10 decrease in the concentrations of KP, PA, CA and EC (5.37, 4.15, 2.97 and 2.67, respectively; all p<0.001). The log10 decreases of SA and EF were 2.27 and 2.11, respectively (all p<0.001). The lowest log10 decrease was observed in BS, AB and FS concentrations (0.25, 0.30 and 0.67, respectively; p<0.001 for BS and AB and p=0.004 for FS). After 14 days, the microbial count of CA, FS, SA, EF, PA and EC further decreased (p=0.006 for FS; p<0.001 for the others).ConclusionKerasave effectively reduced or kept unchanged the microbial concentration of almost all tested strains after 3 days. Thus, this novel medium represents a valuable tool to control the microbial contamination of human donor corneas during hypothermic storage for up to 14 days before transplantation.

Sign in / Sign up

Export Citation Format

Share Document