An abbreviated scheme for identification of Yersinia enterocolitica isolated from food enrichments on CIN (cefsulodin–irgasan–novobiocin) agar

An abbreviated procedure for the biochemical identification of Yersinia enterocolitica isolated from food enrichments on CIN (cefsulodin–irgasan–novobiocin) agar was investigated. A total of 170 colonies resembling Y. enterocolitica in colonial morphology and appearance on CIN agar were selected for identification using API strips. Ninety-three of these isolates were examined with the PathoTec ornithine decarboxylase, Voges–Proskauer, and urease test strips. The PathoTec urease strip alone was adequate for identification of all isolates of Y. enterocolitica. Christensen's urea agar was applied to the remaining 77 isolates and found less specific in that 1 isolate of Enterobacter agglomerons was urease positive along with 10 isolates of Y. enterocolitica. CIN agar is a highly specific medium for isolation of Y. enterocolitica, requiring only Kligler iron agar and urea slants for confirmation of presumptive colonies.

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Simplified scheme for identification of prompt lactose-fermenting members of the Enterobacteriaceae

Journal of Clinical Microbiology ◽

10.1128/jcm.4.6.511-514.1976 ◽

1976 ◽

Vol 4 (6) ◽

pp. 511-514

Author(s):

M J Hicks ◽

K J Ryan

Keyword(s):

Escherichia Coli ◽

Ornithine Decarboxylase ◽

Clinical Microbiology ◽

Clinical Microbiology Laboratory ◽

Microbiology Laboratory ◽

Biochemical Tests ◽

Gram Negative ◽

E Coli ◽

Colonial Morphology ◽

Level Identification

A brief, simplified scheme involving the spot indole test and colonial morphology was evaluated for genus level identification of prompt lactose-fermenting (PLF) members of the Enterobacteriaceae. One hundred and ninety-four consecutive, clinically important PLF gram-negative rods isolated in a clinical microbiology laboratory were identified by this simplified scheme, as well as by standard biochemical tests, and the API 20E (Analytab Products, Inc., Plainview, N.Y.) system. In the simplified scheme a flat, spot indole-positive colony was identified as Escherichia coli. Spot indole-negative organisms forming nucoid colonies were identified as Klebsiella sp. or Enterobacter sp. on the basis of semisolid motility and ornithine decarboxylase tests. Approximately 94% of the study isolates followed reactions typical for E. coli, Klebsiella sp., and Enterobacter sp. as defined by this simplified scheme. When compared with the standard and Analytab Products Inc. identifications, the overall accuracy was 97.4%. The accuracy of identification of E. coli, Klebsiella sp., and Enterobacter sp. was 98.1%, 95.6%, and 87.5%, respectively. This simplified scheme is recommended for identification of selected PLF isolates in the clinical microbiology laboratory.

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To Perform Biochemical Identification of Microorganism by Nitrogen Source Utilization or Urease Test

Basic Techniques in Biochemistry, Microbiology and Molecular Biology - Springer Protocols Handbooks ◽

10.1007/978-1-4939-9861-6_38 ◽

2020 ◽

pp. 145-146

Author(s):

Aakanchha Jain ◽

Richa Jain ◽

Sourabh Jain

Keyword(s):

Nitrogen Source ◽

Urease Test ◽

Biochemical Identification

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PPAR-γ ligands induce G1 cell cycle arrest and reduce ornithine decarboxylase activity of human Barrett's adenocarcinoma cells

Gastroenterology ◽

10.1016/s0016-5085(01)82062-x ◽

2001 ◽

Vol 120 (5) ◽

pp. A415-A415

Author(s):

T TAKASHIMA ◽

Y FUJIWARA ◽

K HIGUCHI ◽

T ARAKAWA ◽

Y YANO ◽

...

Keyword(s):

Cell Cycle ◽

Cell Cycle Arrest ◽

Ornithine Decarboxylase ◽

Decarboxylase Activity ◽

Ornithine Decarboxylase Activity ◽

Ppar Γ ◽

Cycle Arrest ◽

Adenocarcinoma Cells ◽

Barrett’S Adenocarcinoma ◽

G1 Cell Cycle Arrest

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Subcellular localization and light-dark control of ornithine decarboxylase in the unicellular green alga Chlamydomonas reinhardtii

Physiologia Plantarum ◽

10.1034/j.1399-3054.2000.108004353.x ◽

2000 ◽

Vol 108 (4) ◽

pp. 353-360 ◽

Cited By ~ 16

Author(s):

Jürgen Voigt ◽

Bernd Deinert ◽

Peter Bohley

Keyword(s):

Subcellular Localization ◽

Chlamydomonas Reinhardtii ◽

Ornithine Decarboxylase ◽

Green Alga ◽

Dark Control ◽

Unicellular Green Alga

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People With Diabetes Warned About Fake Test Strips

PsycEXTRA Dataset ◽

10.1037/e562722009-007 ◽

2006 ◽

Keyword(s):

Test Strips

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Comparative immune analysis of Yersinia enterocolitica secreted Rps during iron modulated infections

Immunology Letters ◽

10.1016/s0165-2478(97)88557-4 ◽

1997 ◽

Vol 56 (1-3) ◽

pp. 424

Author(s):

S Nikolova

Keyword(s):

Yersinia Enterocolitica

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Sofortige Detektion von Helicobacter pylori mit einer neuen elektrochemischen Messmethode: Feasibility und Vergleich der diagnostischen Wertigkeit mit der Immunhistochemie, dem 13C-Atemtest und dem Helicobacter Urease Test

Zeitschrift für Gastroenterologie ◽

10.1055/s-0030-1267648 ◽

2010 ◽

Vol 48 (10) ◽

Author(s):

H Neumann ◽

S Foertsch ◽

M Vieth ◽

R Kuth ◽

MF Neurath

Keyword(s):

Helicobacter Pylori ◽

Urease Test

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Ornithine Decarboxylase Activity in Cultured Endothelial Cells Stimulated by Serum, Thrombin and Serotonin

Thrombosis and Haemostasis ◽

10.1055/s-0038-1646709 ◽

1978 ◽

Vol 39 (02) ◽

pp. 496-503 ◽

Cited By ~ 8

Author(s):

P A D’Amore ◽

H B Hechtman ◽

D Shepro

Keyword(s):

Endothelial Cells ◽

Ornithine Decarboxylase ◽

Decarboxylase Activity ◽

Aortic Endothelial Cells ◽

Ornithine Decarboxylase Activity ◽

Rate Limiting Step ◽

Bovine Aortic Endothelial Cells ◽

Limiting Step ◽

Rate Limiting ◽

Serum Serotonin

SummaryOrnithine decarboxylase (ODC) activity, the rate-limiting step in the synthesis of polyamines, can be demonstrated in cultured, bovine, aortic endothelial cells (EC). Serum, serotonin and thrombin produce a rise in ODC activity. The serotonin-induced ODC activity is significantly blocked by imipramine (10-5 M) or Lilly 11 0140 (10-6M). Preincubation of EC with these blockers together almost completely depresses the 5-HT-stimulated ODC activity. These observations suggest a manner by which platelets may maintain EC structural and metabolic soundness.

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