scholarly journals Characterization of bacteria isolated from a bleached kraft pulp mill wastewater treatment system

1993 ◽  
Vol 39 (1) ◽  
pp. 13-24 ◽  
Author(s):  
Roberta R. Fulthorpe ◽  
Steven N. Liss ◽  
D. Grant Allen
Keyword(s):  
Wastewater Treatment ◽  
Pulp Mill ◽  
Substrate Utilization ◽  
Treatment System ◽  
Wastewater Treatment System ◽  
Bleached Kraft Pulp ◽  
Large Numbers ◽  
Nickel Copper ◽  
Paper Wastewater ◽  
Utilization Patterns

Water samples from the wastewater treatment system of a bleach kraft mill and from the river that supplies the mill were plated on six different media and culturable isolates were screened for substrate utilization patterns, taxonomic characters, plasmid content, and resistance to ampicillin, streptomycin, kanamycin, tetracycline, naldixic acid, mercury, nickel, copper, cobalt, cadmium, and zinc. A cluster analysis of the substrate utilization profiles and taxonomic characters revealed that Pseudomonas, Acinetobacter, and Acidovorax spp. were common among the culturable isolates from the river, while Ancylobacter aquaticus, Klebsiella spp., and an unknown group of pleomorphic Gram-negative methylotrophs were common among the culturable isolates from the mill treatment system. Of isolates from the settling pond and aerated lagoon, 78 and 64% carried plasmids, while only 56% of isolates from the river carried plasmids. Plasmids were significantly associated with resistance to cadmium but not with any other resistance characters. Large numbers of plasmid-carrying A. aquaticus strains and pleomorphic methylotrophs accounted for high plasmid incidence levels in the mill treatment system, and the ability to dechlorinate simple aliphatic substrates was found in these two groups as well as in one Pseudomonas strain.Key words: pulp and paper, wastewater treatment, chlorinated organics, culturable heterotrophs, methylotrophs, plasmids.

An Assessment of Variability of Pulp Mill Wastewater Treatment System Bacterial Communities using Molecular Methods

2003 ◽  
Vol 38 (2) ◽  
pp. 227-242 ◽  
Author(s):  
Christopher J.O. Baker ◽  
Roberta R. Fulthorpe ◽  
Kimberley A. Gilbride
Keyword(s):  
Wastewater Treatment ◽  
Bacterial Communities ◽  
Intergenic Spacer ◽  
Pulp Mill ◽  
Treatment System ◽  
Computer Assisted ◽  
Dna Fingerprints ◽  
Similarity Coefficients ◽  
Paper Wastewater ◽  
The Stability

Abstract The DNA fingerprinting techniques, 16S-restriction fragment length polymorphism (16S-RFLP), ribosomal intergenic spacer analysis (RISA) and repetitive extragenic palindrome PCR (Rep-PCR), were used for analyzing the bacterial communities of seven pulp and paper wastewater treatment systems. All three methods generate DNA fingerprints that can be compared using the computer-assisted program, Gelcompar©. Community similarity coefficients were based on quantitative determinations of both the positions of the DNA bands and the band intensities in order to compare the relative differences in the populations. Unique 16S-RFLP DNA fingerprints were observed for each mill suggesting that individual mills contained phylogenetically different communities. However this method was not sensitive enough to detect differences within a mill treatment system from different locations or from different sampling times. The RISA method, which generated more complex fingerprints than 16S-RFLP, could, for some mills, discern differences between samples. The Rep-PCR technique, however, showed the highest degree of resolution and produced not only distinct patterns for each mill but also distinct fingerprints for the temporal and spatial samples from some of the treatment systems. The sensitivity of this method might potentially be used to monitor the stability of the bacterial community within a secondary treatment system.

scholarly journals The Long Experience of Anaerobic Wastewater Treatment System for Pulp Mill Wastewater

2013 ◽  
Vol 67 (5) ◽  
pp. 512-516
Author(s):  
Haruyuki Chiku ◽  
Takanori Takeshige ◽  
Atsushi Shimamoto ◽  
Nagako Watanabe ◽  
Atsushi Nakano
Keyword(s):  
Wastewater Treatment ◽  
Pulp Mill ◽  
Treatment System ◽  
Anaerobic Wastewater Treatment ◽  
Wastewater Treatment System

Performance of hybrid small wastewater treatment system consisting of jet mixed separator and rotating biological contactor

1997 ◽  
Vol 35 (6) ◽  
pp. 63-70 ◽  
Author(s):  
Yoshimasa Watanabe ◽  
Yoshihiko Iwasaki
Keyword(s):  
Wastewater Treatment ◽  
Electric Power ◽  
Hybrid System ◽  
Municipal Wastewater ◽  
Treatment Plant ◽  
Treatment System ◽  
Municipal Wastewater Treatment ◽  
Wastewater Treatment System ◽  
Pre Treatment ◽  
Organic Particles

This paper describes a pilot plant study on the performance of a hybrid small municipal wastewater treatment system consisting of a jet mixed separator(JMS) and upgraded RBC. The JMS was used as a pre-treatment of the RBC instead of the primary clarifier. The treatment capacity of the system was fixed at 100 m3/d, corresponding to the hydraulic loading to the RBC of 117 L/m2/d. The effluent from the grid chamber at a municipal wastewater treatment plant was fed into the hybrid system. The RBC was operated using the electric power produced by a solar electric generation panel with a surface area of 8 m2 under enough sunlight. In order to reduce the organic loading to the RBC, polyaluminium chloride(PAC) was added to the JMS influent to remove the colloidal and suspended organic particles. At the operational condition where the A1 dosage and hydraulic retention time of the JMS were fixed at 5 g/m3 and 45 min., respectively, the average effluent water quality of hybrid system was as follows: TOC=8 g/m3, Total BOD=8 g/m3, SS=8 g/m3, Turbidity=6 TU, NH4-N=7 g/m3, T-P=0.5 g/m3. In this operating condition, electric power consumption of the RBC for treating unit volume of wastewater is only 0.07 KWH/m3.

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