Transient-Phase and Steady-State Kinetics for Inhibited Enzyme Systems. I. Single-Intermediate Mechanisms

1973 ◽  
Vol 51 (6) ◽  
pp. 815-821 ◽  
Author(s):  
Nasrat H. Hijazi ◽  
Keith J. Laidler
Keyword(s):  
Steady State ◽  
Enzyme Concentration ◽  
Experimental Results ◽  
Transient Phase ◽  
Steady State Analysis ◽  
Noncompetitive Inhibition ◽  
Enzyme Systems ◽  
Steady State Kinetics ◽  
Inhibited Enzyme ◽  
State Analysis

Equations for the pre-steady state and the steady state are derived for enzyme systems in which enzyme E, substrate A, and inhibitor Q are present, the enzyme concentration being lower than the substrate and inhibitor concentrations. It is assumed that the mechanism involves a single intermediate EA. Equations for competitive, anticompetitive, and pure noncompetitive inhibition are derived. When the inhibition is reversible the transient phase is followed by the establishment of a steady state. Analysis of experimental results is discussed for each type of inhibition. If the inhibition is irreversible, there is no steady state.

Transient-Phase and Steady-State Kinetics for Inhibited Enzyme Systems. II. Double-Intermediate Mechanisms

1973 ◽  
Vol 51 (6) ◽  
pp. 822-831 ◽  
Author(s):  
Nasrat H. Hijazi ◽  
Keith J. Laidler
Keyword(s):  
Steady State ◽  
Rate Constants ◽  
Enzyme Concentration ◽  
Transient Phase ◽  
Enzyme Systems ◽  
Steady State Kinetics ◽  
Inhibited Enzyme

Equations for the pre-steady state and the steady state are derived for enzyme systems in which the enzyme E, the substrate A, and an inhibitor Q are present together, the enzyme concentration being much lower than the concentrations of A and Q. Various mechanisms are considered, ail of them involving two intermediates EA and EA′ (e.g. an acyl enzyme). When the inhibition is reversible the transient phase is followed by the establishment of a steady state. It is shown how experimental pre-steady-state and steady-state results can be analyzed to obtain rate constants, including those for the binding of inhibitor. If the binding of inhibitor is irreversible there is no steady state.

Transient-Phase and Steady-State Kinetics for Enzyme Systems Involving Two Substrates

1973 ◽  
Vol 51 (6) ◽  
pp. 832-840 ◽  
Author(s):  
Nasrat H. Hijazi ◽  
Keith J. Laidler
Keyword(s):  
Steady State ◽  
Ternary Complex ◽  
Transient Phase ◽  
Complex Mechanism ◽  
State Equations ◽  
Enzyme Systems ◽  
Steady State Kinetics ◽  
Ping Pong ◽  
The Individual ◽  
Individual Rate

The transient-phase and steady-state equations are derived for four enzyme mechanisms involving two substrates, namely (1) Theorell–Chance mechanism, (2) ping pong bi bi mechanism, (3) ordered ternary-complex mechanism, and (4) random ternary-complex mechanism. In each case, a discussion is presented of the way in which the individual rate constants can be separated on the basis of experimental transient-phase investigations.

Bridgeless SEPIC PFC Converter

2013 ◽  
Vol 313-314 ◽  
pp. 51-54
Author(s):  
Hyun Lark Do
Keyword(s):  
Steady State ◽  
Power Factor ◽  
Experimental Results ◽  
Unity Power Factor ◽  
Steady State Analysis ◽  
Coupled Inductors ◽  
State Analysis

A new bridgeless powerfactor correction (PFC) converter is proposed in this paper. The proposed converter isbased on single-ended primary inductor converter (SEPIC). In the proposed converter, conductionlosses are reduced and efficiency is improved by eliminating bridge diodes. In addition, input currentripple is significantly reduced by utilizing coupled inductors. Like a conventional SEPIC PFCconverter, the proposed converter provides almost unity power factor (PF). Steady-state analysis ofthe proposed converter is performed. Experimental results based on a prototype are alsoprovided to verify the effectiveness and feasibility of the proposed converter.

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