The reactivity to N-ethyl maleimide of the subunits of cytochrome oxidase
1977 ◽
Vol 55
(9)
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pp. 988-994
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Keyword(s):
Beef heart cytochrome oxidase (EC 1.9.3.1) prepared in this laboratory consistently presents 10 Coomassie blue staining zones on SDS–polyacrylamide gel electrophoresis. At pH 7.0 only two of these polypeptides (III and VIa) are labelled by radioactive N-ethyl maleimide (NEM). The labelling of VIa is variable and correlates with the activity of particular oxidase preparations. When cytochrome oxidase is isolated from alkylated membranes, either mitochondria or electron transport particles, polypeptide VIa is found not to be labelled; polypeptide III is more strongly labelled than when isolated oxidase is alkylated, and label now appears in polypeptide I which is not alkylated upon treatment of isolated oxidase with NEM.
1987 ◽
Vol 33
(10)
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pp. 1886-1887
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1996 ◽
Vol 45
(1)
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pp. 6-9
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1983 ◽
Vol 61
(7)
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pp. 756-763
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1992 ◽
Vol 4
(2)
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pp. 249
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2017 ◽
Vol 5
(2)
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pp. 31-38
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1971 ◽
Vol 43
(1)
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pp. 156-161
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1981 ◽
1979 ◽
Vol 96
(2)
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pp. 263-278
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