Structural analysis of the specific polysaccharide of Streptococcus pneumoniae type 9L (American type 49)

1984 ◽  
Vol 62 (12) ◽  
pp. 1309-1320 ◽  
Author(s):  
James C. Richards ◽  
Malcolm B. Perry ◽  
Peter J. Kniskern
Keyword(s):  
Streptococcus Pneumoniae ◽  
Nitrous Acid ◽  
Capsular Polysaccharide ◽  
Glucuronic Acid ◽  
Optical Rotation ◽  
Periodate Oxidation ◽  
Part D ◽  
Partial Acid Hydrolysis ◽  
Specific Polysaccharide ◽  
Structure Formula

The specific capsular polysaccharide produced by Streptococcus pneumoniae type 9L (American type 49) is composed of D-galactose (one part), D-glucose (one part), D-glucuronic acid (one part), 2-acetamido-2-deoxy-D-mannose (one part) and 2-acetamido-2-deoxy-D-glucose (one part). Partial acid hydrolysis, periodate oxidation, nitrous acid deamination, optical rotation, methylation and 13C and 1H nuclear magnetic resonance studies showed that the polysaccharide is an unbranched high molecular weight linear polymer of a repeating pentasaccharide unit having the structure:[Formula: see text]

The structure of the specific capsular polysaccharide of Streptococcus pneumoniae type 11F (American type 11)

1985 ◽  
Vol 63 (9) ◽  
pp. 953-968 ◽  
Author(s):  
James C. Richards ◽  
Malcolm B. Perry ◽  
Peter J. Kniskern
Keyword(s):  
Streptococcus Pneumoniae ◽  
Capsular Polysaccharide ◽  
Optical Rotation ◽  
Periodate Oxidation ◽  
Capsular Polysaccharides ◽  
Glycerol Phosphate ◽  
Part D ◽  
Magnetic Resonance Studies ◽  
Specific Polysaccharide ◽  
Structure Formula

The specific polysaccharide of Streptococcus pneumoniae type 11F (American type 11) is composed of 2-acetamido-2-deoxy-D-glucose (one part), D-glucose (one part), D-galactose (two parts), ribitol (one part), phosphate (one part), and O-acetyl (two parts). Hydrolysis, dephosphorylation, periodate oxidation, methylation, optical rotation, and 1H and 13C nuclear magnetic resonance studies showed that the polysaccharide is an unbranched linear polymer of a ribitol-phosphate substituted repeating tetrasaccharide unit having the structure:[Formula: see text]The specific capsular polysaccharides of S. pneumoniae type 11B and 11C (American types 76 and 53) were found to have the same tetrasaccharide repeating unit as the 11F polysaccharide, but differed from it in their mode of O-acetylation and the replacement of the ribitol phosphate by glycerol phosphate in the 11C specific polysaccharide.

Structure of the acidic capsular polysaccharide of Cryptococcus laurentii (NRRL Y-1401)

1982 ◽  
Vol 60 (2) ◽  
pp. 124-130 ◽  
Author(s):  
Malcolm B. Perry ◽  
Ann C. Webb
Keyword(s):  
Molecular Weight ◽  
Capsular Polysaccharide ◽  
Glucuronic Acid ◽  
Optical Rotation ◽  
Periodate Oxidation ◽  
Regular Structure ◽  
Cryptococcus Laurentii ◽  
Partial Acid Hydrolysis ◽  
Magnetic Resonance Studies ◽  
Structure Formula

The capsular polysaccharide produced by Cryptococcus laurentii (NRRL Y-1401) is composed of D-mannose (3 mol), D-glucuronic acid (1 mol), D-xylose (1 mol), and O-acetyl (~1 mol). Methylation, periodate oxidation, partial acid hydrolysis, optical rotation, and nuclear magnetic resonance studies showed that the polysaccharide is a high molecular weight branched polymer of regular structure having a repeating pentasaccharide unit with the structure:[Formula: see text]

The specific capsular polysaccharide of Streptococcus pneumoniae type 9V

1981 ◽  
Vol 59 (7) ◽  
pp. 524-533 ◽  
Author(s):  
Malcolm B. Perry ◽  
Virginia Daoust ◽  
Dennis J. Carlo
Keyword(s):  
Molecular Weight ◽  
Nuclear Magnetic Resonance ◽  
Streptococcus Pneumoniae ◽  
Capsular Polysaccharide ◽  
Glucuronic Acid ◽  
Optical Rotation ◽  
Periodate Oxidation ◽  
Partial Hydrolysis ◽  
Part D ◽  
Magnetic Resonance Studies

The specific capsular polysaccharide produced by Streptococcus pneumoniae type 9V (American type 68) is composed of D-glucuronic acid (1 part), D-galactose (1 part), 2-acetamido-2-deoxy-D-mannose (1 part), D-glucose (2 parts), and O-acetyl (1.6 parts). Methylation, periodate oxidation, optical rotation, and nuclear magnetic resonance studies, and partial hydrolysis showed that the polysaccharide is an unbranched high molecular weight linear polymer of a partially O-acetylated pentasaccharide repeating unit having the structure indicated below.[Formula: see text]

The specific capsular polysaccharide of Streptococcus pneumoniae type 15A (American type 30)

1984 ◽  
Vol 62 (2-3) ◽  
pp. 151-161 ◽  
Author(s):  
Martine Caroff ◽  
Malcolm B. Perry
Keyword(s):  
Molecular Weight ◽  
Nuclear Magnetic Resonance ◽  
Streptococcus Pneumoniae ◽  
Magnetic Resonance ◽  
High Molecular Weight ◽  
Capsular Polysaccharide ◽  
Optical Rotation ◽  
Periodate Oxidation ◽  
Magnetic Resonance Studies ◽  
Structure Formula

The specific capsular polysaccharide of Streptococcus pneumoniae type 15A (American type 30) is composed of D-galactose (three parts), D-glucose (one part), 2-acetamido-2-deoxy-D-glucose (one part), phosphate (one part), and glycerol (one part). Hydrolysis, periodate oxidation, methylation, optical rotation, and nuclear magnetic resonance studies showed that the polysaccharide is a high molecular weight linear polymer of a pentasaccharide repeating unit having the structure:[Formula: see text]

Structure of the specific capsular polysaccharide of Streptococcus pneumoniae type 23F (American type 23)

1988 ◽  
Vol 66 (7) ◽  
pp. 758-771 ◽  
Author(s):  
James C. Richards ◽  
Malcolm B. Perry
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Streptococcus Pneumoniae ◽  
High Resolution ◽  
Capsular Polysaccharide ◽  
Optical Rotation ◽  
Periodate Oxidation ◽  
Partial Hydrolysis ◽  
Composition Analysis ◽  
Part D ◽  
Magnetic Resonance Studies

The specific capsular polysaccharide of Streptococcus pneumoniae serotype 23F (American type 23) is composed of a repeating tetrasaccharide unit containing D-glucose (one part), D-galactose (one part), L-rhamnose (two parts), glycerol (one part), and phosphate (one part). By composition analysis, optical rotation, partial hydrolysis, periodate oxidation, methylation, and high-resolution 1H and 13C nuclear magnetic resonance studies, the elucidated unambiguous structure was in agreement with our earlier proposal but is at variance with structures proposed later by other authors. The structure of the type 23F pneumococcal polysaccharide is[Formula: see text]

Structural analysis of the specific capsular polysaccharide of Streptococcuspneumoniae type 22F

1989 ◽  
Vol 67 (6) ◽  
pp. 1038-1050 ◽  
Author(s):  
James C. Richards ◽  
Malcolm B. Perry ◽  
Peter J. Kniskern
Keyword(s):  
Capsular Polysaccharide ◽  
Glucuronic Acid ◽  
Periodate Oxidation ◽  
Resonance Spectroscopy ◽  
Methylation Analysis ◽  
Chemical Shift Correlation ◽  
Structure Formula ◽  
Correlation Techniques ◽  
Heteronuclear Chemical Shift ◽  
C Nmr

The structure of the specific capsular polysaccharide produced by Streptococcuspneumoniae type 22F (American type 22) was investigated by high-field 1H and 13C nuclear magnetic resonance spectroscopy, composition, methylation analysis, and periodate oxidation studies. The polysaccharide was found to be a high molecular weight acidic polymer composed of D-glucose, D-galactose, D-glucuronic acid, and L-rhamnose residues to form a regular repeating hexasaccharide unit having the structure[Formula: see text]in which the β-L-rhamnopyranosyl residues were substituted by O-acetyl groups in 80% of the repeating units. The 1H and 13C nmr resonances of the O-deacetylated type 22F polysaccharide were completely assigned by application of two-dimensional homo- and heteronuclear chemical shift correlation techniques. Keywords: Streptococcuspneumoniae polysaccharide, NMR analysis.

The specific capsular polysaccharide of Streptococcus pneumoniae type 20

1983 ◽  
Vol 61 (4) ◽  
pp. 178-190 ◽  
Author(s):  
James C. Richards ◽  
Malcolm B. Perry ◽  
Dennis J. Carlo
Keyword(s):  
Molecular Weight ◽  
Nuclear Magnetic Resonance ◽  
Streptococcus Pneumoniae ◽  
Magnetic Resonance ◽  
High Molecular Weight ◽  
Capsular Polysaccharide ◽  
Optical Rotation ◽  
Periodate Oxidation ◽  
High Molecular Weight Polymer ◽  
Molecular Weight Polymer

The specific capsular polysaccharide produced by Streptococcus pneumoniae type 20 is composed of D-glucose (three parts), D-galactose (two parts), 2-acetarnido-2-deoxy-D-glucose (one part), phosphate (one part), and O-acetyl (approximately two parts). Methylation, periodate oxidation, optical rotation, nuclear magnetic resonance, partial and enzymic hydrolyses, and chromatographic studies showed that the polysaccharide is a high molecular weight polymer of partially O-acetylated hexasaccharide repeating units linked by monophosphate, having the structure indicated below:[Formula: see text]

The specific capsular polysaccharide of Streptococcus pneumoniae type 33F

1984 ◽  
Vol 62 (8) ◽  
pp. 666-677 ◽  
Author(s):  
James C. Richards ◽  
Malcolm B. Perry ◽  
Peter J. Kniskern
Keyword(s):  
Molecular Weight ◽  
Nuclear Magnetic Resonance ◽  
Streptococcus Pneumoniae ◽  
Capsular Polysaccharide ◽  
Periodate Oxidation ◽  
Partial Hydrolysis ◽  
High Molecular Weight Polymer ◽  
Molecular Weight Polymer ◽  
Magnetic Resonance Studies ◽  
Structure Formula

The specific capsular polysaccharide of Streptococcus pneumoniae type 33F (American type 70) is composed of D-galactose (5 parts), D-glucose (1 part), and O-acetyl (ca. 0.4 parts). Periodate oxidation, partial hydrolysis, and 1H and 13C nuclear magnetic resonance studies showed that the polysaccharide is a high molecular weight polymer of a repeating hexasaccharide unit having the structure:[Formula: see text]

Structural studies of the capsular polysaccharide from Streptococcus pneumoniae Type 12F

1981 ◽  
Vol 59 (14) ◽  
pp. 2081-2085 ◽  
Author(s):  
Karin Leontein ◽  
Bengt Lindberg ◽  
Jörgen Lönngren
Keyword(s):  
Streptococcus Pneumoniae ◽  
Nmr Spectroscopy ◽  
Capsular Polysaccharide ◽  
Structural Studies ◽  
Methylation Analysis ◽  
Mannuronic Acid ◽  
Structure Formula

The capsular polysaccharide from Streptococcus pneumoniae type 12F is composed of D-glucosyl, D-galactosyl, 2-acetamido-2-deoxy-D-galactosyl, 2-acetamido-2,6-dideoxy-L-galactosyl, and 2-acetamido-2-deoxy-D-mannuronic acid residues in the proportions 2:1:1:1:1. The main structural evidence was adduced from nmr spectroscopy, methylation analysis, and specific degradations whereby it could be concluded that the polysaccharide is composed of hexasaccharide repeating-units having the structure:[Formula: see text]

Structural elucidation of the capsular polysaccharide of Streptococcus pneumoniae type 9N

1983 ◽  
Vol 61 (10) ◽  
pp. 1102-1107 ◽  
Author(s):  
Karl-Gunnar Rosell ◽  
Harold J. Jennings
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Streptococcus Pneumoniae ◽  
Magnetic Resonance ◽  
Capsular Polysaccharide ◽  
Glucuronic Acid ◽  
Cross Reactivity ◽  
Structural Elucidation ◽  
Molar Ratio ◽  
The Cross ◽  
Nuclear Magnetic

The specific capsular polysaccharide of Streptococcus pneumoniae type 9N (American type 9) contains D-glucose, D-glucuronic acid, 2-acetamido-2-deoxy-D-glucose, and 2-acetamido-2-deoxy-D-mannose in the molar ratio of 2:1:1:1. Accumulated data from spectroscopic (13C and 1H nuclear magnetic resonance) and methylation analyses of the native and specifically degraded polysaccharide indicated that it was linear and composed of the following pentasaccharide repeating unit; —4)-α-D-GlcpA-(1 → 3)-α-D-Glcp-(1 → 3)-β-D-ManpNAc-(1 → 4)-α-D-Glcp-(1 → 4)-β-D-GlcpNAc(1 →. Structural regions in the type 9N polysaccharide common to those of types 9A, 9L, and 9V have been identified which account for the cross-reactivity of this groups of polysaccharides.

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