THE PARTIAL SEPARATION OF Na22 FROM Na24 BY ION EXCHANGE CHROMATOGRAPHY

1956 ◽  
Vol 34 (1) ◽  
pp. 65-74 ◽  
Author(s):  
R. H. Betts ◽  
W. E. Harris ◽  
Margaret D. Stevenson
Keyword(s):  
Hydrochloric Acid ◽  
Ion Exchange ◽  
Cation Exchange ◽  
Exchange Resin ◽  
Isotope Effects ◽  
Cation Exchange Resin ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Partial Separation ◽  
Chloride Salts

Mixtures of the radionuclides Na22 and Na24, as the chloride salts, have been partially separated by chromatographic methods. A column 102 cm. in length containing the cation exchange resin Dowex 50 was used. The eluant was 0.7 M hydrochloric acid. Na22 is held more strongly by the resin than is Na24. The most effective separations were obtained at 25 °C and 5.5 °C.; experiments at 48 °C and 68 °C showed much smaller isotope effects.

The non-protein nitrogen composition of grass silages: I. The estimation of the basic amino acids and non-volatile amines by chromatography on a weak cation exchange resin

1969 ◽  
Vol 72 (3) ◽  
pp. 459-466 ◽  
Author(s):  
A. D. Hughes
Keyword(s):  
Amino Acids ◽  
Ion Exchange ◽  
Cation Exchange ◽  
Exchange Resin ◽  
Cation Exchange Resin ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Protein Nitrogen ◽  
Weak Cation Exchange

SUMMARYInvestigations into the non-protein nitrogen composition of grass silages using the 50 cm strong cation-exchange column of Spackman, Stein & Moore (1958) to determine the basic amino acids led to difficulties in the determination of ethanolamine in the presence of high concentrations of ammonia, and of histidine in the presence of δ amino-n-valeric acid. An alternative technique for the ion exchange chromatography and estimation of histidine, lysine, ornithine, ethanolamine, arginine and ammonia on a weak cation-exchange resin has been developed. This method enables small amounts of ethanolamine to be determined in the presence of large amounts of ammonia and values for the ethanolamine content of a number of silage samples are presented. When used in conjunction with the technique of Spackman et al. (1958) the δ-amino-n-valeric acid content of grass silages could also be determined in the presence of histidine.The estimation of amines produced by the microbial decomposition of herbage proteins during ensiling has previously involved their initial separation from the amino acids followed by quantitative partition chromatography. An alternative method for the estimation of these amines by ion-exchange chromatography on a weak cation-exchange resin is described. This method permits the colorimetric determination of β-phenylethylamine, tyramine, tryptamine, 5-hydroxytryptamine, putrescine, cadaverine and histamine without interference from the amino acids. The efficiency of this technique has been investigated using standard solutions of the naturally occurring amines and samples of good quality and of high pH spoilt silages.

scholarly journals Separation of 1,3-Propanediol from Aqueous Solutions by Ion Exchange Chromatography

2014 ◽  
Vol 16 (2) ◽  
pp. 82-86 ◽  
Author(s):  
Beata Rukowicz ◽  
Ireneusz Miesiąc ◽  
Krzysztof Alejski
Keyword(s):  
Ion Exchange ◽  
Cation Exchange ◽  
Renewable Resources ◽  
Exchange Resin ◽  
Fermentation Broth ◽  
Cation Exchange Resin ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Sorption Method ◽  
Separation Of Mixtures

Abstract 1,3-propanediol is a promising monomer with many applications and can be produced by bioconversion of renewable resources. The separation of this product from fermentation broth is a difficult task. In this work, the application of cation exchange resin for the separation of 1,3-propanediol from model aqueous solution was examined. The best effect of separation of 1,3-propanediol from glycerol using sorption method was obtained for H+ resin form, although the observed partition coefficient of 1,3-propanediol was low. On the basis of the results of the sorption of 1,3-propanediol, the ionic forms of the resin were selected and used in the next experiments (H+, Ca2+, Ag+, Na+, Pb2+, Zn2+). The best results in ion exchange chromatography were obtained for cation exchange resin in H+ and Ca2+ form. The use of smaller particle size of resin and a longer length of the column allows to obtain better separation of mixtures.

Temperature Dependence of Lithium Isotope Effects in Ion Exchange Electromigration

1987 ◽  
Vol 42 (7) ◽  
pp. 709-712
Author(s):  
Yasuhiko Fujii ◽  
Morikazu Hosoe ◽  
Okamoto Makoto
Keyword(s):  
Ion Exchange ◽  
Cation Exchange ◽  
Isotope Effects ◽  
Isotope Separation ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Separation Coefficient ◽  
Lithium Isotope ◽  
Increasing Temperature ◽  
Exchange Membrane

The isotope separation coefficient of lithium electromigration through a cation exchange membrane is determined at 6, 11, 20 and 40 °C . It is found that this coefficient increases with temperature while the slope of the isotope distribution in the band decreases with increasing temperature. These tendencies are opposite to those usually observed in ion exchange chromatography.

Simplified radioimmunoassay of urinary drugs of abuse adsorbed on ion-exchange papers.

1977 ◽  
Vol 23 (10) ◽  
pp. 1921-1924 ◽  
Author(s):  
G J Alexander ◽  
S Machiz
Keyword(s):  
Ion Exchange ◽  
Cation Exchange ◽  
Drugs Of Abuse ◽  
Exchange Resin ◽  
Cation Exchange Resin ◽  
Screening Procedure ◽  
Central Laboratory ◽  
Small Disc

Abstract A convenient screening procedure for presence of drugs of abuse in urine consists of two steps: adsorption of the drugs from urine onto a paper loaded with cation-exchange resin and detection of the adsorbed drugs by direct radioimmunoassay. The first step can be performed in the field, the second in a central laboratory. Storage and transport to the laboratory are simplified because specimens adsorbed on dried paper are stable and can be sent in letter-mail. In the laboratory, a small disc of the ion-exchange paper is exposed to antigen and antibody, rinsed, and tested for radioactivity. Discs treated with positive urines are more radioactive than discs from negative urines.

False-negative results for urinary phenothiazines and imipramine in Forrest's qualitative assays.

1980 ◽  
Vol 26 (2) ◽  
pp. 345-347 ◽  
Author(s):  
G P James ◽  
M H DJang ◽  
H H Hamilton
Keyword(s):  
Ascorbic Acid ◽  
Ion Exchange ◽  
Exchange Resin ◽  
Anion Exchange Resin ◽  
False Negative ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Negative Results ◽  
False Negative Results

Abstract When a series of patients' urine samples supplemented in vitro with chlorpromazine or imipramine was assayed with the Forrest qualitative assays, we observed an occasional false-negative result, which we found was attributable to interference by ascorbic acid. It interferes with the reagent, not with the analytes, in both assays. We easily eliminated this interference with the phenothiazine test by using an anion-exchange resin. Eliminating the interference with the assay for imipramine, however, is more difficult; false-negative results can be obtained even after ion-exchange chromatography if the imipramine concentration is less than 50 mg/L.

Ion Exchange Method for Rapid Determination of Alkalinity of Water-Soluble Tea Ash Containing High Levels of Manganese

1980 ◽  
Vol 63 (3) ◽  
pp. 460-461
Author(s):  
Saidul Z Qureshi ◽  
Fadhil M Najib ◽  
Fahmi A Mohammed
Keyword(s):  
Ion Exchange ◽  
Cation Exchange ◽  
Exchange Resin ◽  
Rapid Determination ◽  
Cation Exchange Resin ◽  
Water Soluble ◽  
Exchange Method ◽  
Strong Cation Exchange ◽  
Ion Exchange Method

Abstract An ion exchange method to determine the alkalinity of water-soluble tea ash containing high levels of manganese is described. A chromatographic column containing a strong cation exchange resin (20–50 mesh) in Na+ form, with a bed volume of 5 mL is used. The present ion exchange method is compared to pH titrations and also to the official AOAC methods (31.012, 31.015, 31.016). Results with the new method are accurate and precise.

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