Condensation de la poly-L-lysine et de la poly- L-ornithine avec des dérivés de bases puriques et de sulfamides

1969 ◽  
Vol 47 (19) ◽  
pp. 3641-3646 ◽  
Author(s):  
Louis Berlinguet ◽  
Jacky Gautier
Keyword(s):  
Aqueous Solution ◽  
Amino Acids ◽  
Free Amino ◽  
Sulfonyl Chloride ◽  
Amino Groups ◽  
Carbodiimide Method

Free ε-amino groups of poly-L-lysine and poly-L-ornithine were alkylated in aqueous solution with 6-chloropurine and 6-chloropurine-riboside, were acylated with 4-acetylamino benzene sulfonyl chloride, and were condensed with N-(4-acetylamino benzene sulfonyl)-glycine using carbodiimide method. Approximately 50% of the free amino groups of the basic polypetides were substituted. The same method was used to prepare poly-α-amino acids bearing radioactive substituants.

Automated Determination of Free Amino Groups in Serum and Plasma Using 2,4,6-Trinitrobenzene Sulfonate

1969 ◽  
Vol 15 (9) ◽  
pp. 891-901 ◽  
Author(s):  
D W Palmer ◽  
T Peters
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Clinical Applications ◽  
Amino Groups ◽  
Automated Method ◽  
Total Free Amino Acids ◽  
Colored Complexes ◽  
Automated Determination

Abstract A simple automated method is described for determining the level of total free amino acids in the blood. The method utilizes the AutoAnalyzer, and is based on the formation of colored complexes by uniting free amino groups with 2,4,6-trinitrobenzene sulfonate (TNBS). Proteins do not interfere because the free amino acids are first separated by dialysis. Characteristics of the reaction and potential clinical applications of the procedure are discussed.

scholarly journals The Free Amino Groups of the Proteus Flagella Protein. Determination of Dinitrophenyl Amino Acids Using Paper Chromatography.

1953 ◽  
Vol 7 ◽  
pp. 335-339 ◽  
Author(s):  
Claes Weibull ◽  
Artturi I. Virtanen ◽  
J. K. Miettinen ◽  
Artturi I. Virtanen ◽  
Nils Andreas Sörensen
Keyword(s):  
Amino Acids ◽  
Paper Chromatography ◽  
Free Amino ◽  
Amino Groups ◽  
Protein Determination

scholarly journals The reaction of 2,4,6-trinitrobenzenesulphonic acid with amino acids, peptides and proteins

1968 ◽  
Vol 108 (3) ◽  
pp. 383-391 ◽  
Author(s):  
R. B. Freedman ◽  
G. K. Radda
Keyword(s):  
Amino Acids ◽  
Glutamate Dehydrogenase ◽  
Free Amino ◽  
Reactive Species ◽  
Second Order ◽  
Amino Group ◽  
Exponential Rate ◽  
Amino Groups ◽  
Sh Group ◽  
Kinetics Of

1. The kinetics of the reaction of 2,4,6-trinitrobenzenesulphonic acid with various amino acids, peptides and proteins were studied by spectrophotometry. 2. The reaction of the α- and ∈-amino groups in simple amino acids was found to be second-order, and the unprotonated amino group was shown to be the reactive species. 3. By allowing for the concentration of unreactive −NH3+ group, intrinsic reactivities for the free amino groups were derived and shown to be correlated with the basicities. 4. The SH group of N-acetylcysteine was found to be more reactive to 2,4,6-trinitrobenzenesulphonic acid than most amino groups. 5. The reactions of insulin, chymotrypsinogen and ribonuclease with 2,4,6-trinitrobenzenesulphonic acid were analysed in terms of three exponential rate curves, each referring to one or more amino groups of the proteins. 6. The reaction of lysozyme with 2,4,6-trinitrobenzenesulphonic acid was found to display an acceleration effect. 7. From the reaction of 2,4,6-trinitrobenzenesulphonic acid with glutamate dehydrogenase at several enzyme concentrations, it was possible to discern two sets of amino groups of different reactivity, and to show that the number of groups in each set was decreased by aggregation of the enzyme.

Measurement of proteolysis in cheese: relationship between phosphotungstic acid-soluble N fraction by Kjeldahl and 2,4,6- trinitrobenzenesulphonic acid-reactive groups in water-soluble N

1994 ◽  
Vol 61 (3) ◽  
pp. 437-440 ◽  
Author(s):  
Yvette Bouton ◽  
Remy Grappin
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Phosphotungstic Acid ◽  
Water Soluble ◽  
Hydroxyl Groups ◽  
Amino Groups ◽  
Reactive Groups ◽  
Primary Amino

Free amino groups produced during cheese ripening are used to indicate the extent of cheese proteolysis. Several studies have shown a high correlation between the level of free amino acids and the flavour of Gouda (Aston et al. 1983) or Comté (Grappin & Berdagué, 1989). Measurement of the level of free amino acids seems useful for the investigation of flavour chemistry in cheese (Lemieux et al. 1990). The determination of N fractions is often used to estimate the degree of proteolysis in cheese, but since this procedure is laborious and time consuming several attempts have been made to replace it by more rapid methods (Ardö & Meisel, 1991). Since its introduction by Satake et al. (1960), the 2,4,6-trinitrobenzenesulphonic acid (TNBS) method has been widely used for the determination of free amino groups. Because TNBS does not react with the imino groups of histidine and proline or the hydroxyl groups of tyrosine, serine or threonine, it has been accepted as a selective reagent for primary amino groups (Burger, 1974). Measurement of N by Kjeldahl in the phosphotungstic acid (PTA)–sulphuric acid extract (Gripon et al. 1975) estimates the N of free amino acids and low molecular mass peptides. The purpose of this study was to compare the TNBS and PTA-soluble N methods in order to find out whether the TNBS procedure can replace that of PTA-soluble N in the determination of a cheese proteolysis index.

scholarly journals THE RIBONUCLEOPROTEIN NATURE OF LARGE PARTICLES IN THE MEIOSPORANGIA OF ALLOMYCES

1957 ◽  
Vol 3 (6) ◽  
pp. 879-888 ◽  
Author(s):  
Edward S. Rorem ◽  
Leonard Machlis
Keyword(s):  
Aqueous Solution ◽  
Amino Acids ◽  
Oleic Acid ◽  
Free Amino Acids ◽  
Free Amino ◽  
Large Particles ◽  
Significant Change

Particles averaging 3 to 4 µ in diameter, which are called chromospheres and fill the immature meiosporangia of the watermold Allomyces, were isolated and analyzed. The preparations were obtained by repeated centrifugations or by passage of the homogenate into a column of sand saturated with oleic acid, followed by selective elution of the chromospheres with alternate layers of oleic acid and an aqueous solution. The chromospheres contain approximately 12 per cent RNA, no DNA, and 60 per cent protein. It was concluded that they are pure or nearly pure ribonucleoprotein. Analysis of meiosporangia with chromospheres and after the chromospheres have disappeared showed no significant change in RNA or free amino acids. It was concluded that chromosphere disappearance is a fragmentation into small granules. The relation of chromospheres to postmeiotic chromospheres and nuclear caps is discussed. Speculation as to the function of these bodies is presented.

Betaines and free amino acids in salt stressed vitroplants and winter resting buds of Populus trichocarpa x deltoides

1991 ◽  
Vol 83 (1) ◽  
pp. 136-143 ◽  
Author(s):  
L. Bray ◽  
D. Chriqui ◽  
K. Gloux ◽  
D. Le Rudulier ◽  
M. Meyer ◽  
...  
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Populus Trichocarpa

COMPARATIVE STUDIES OF THE POTENTIATION OF CORTICOTROPHIN ACTIVITY BY SEVERAL INACTIVE DERIVATIVES

1963 ◽  
Vol 42 (2) ◽  
pp. 209-213 ◽  
Author(s):  
Arthur I. Cohen ◽  
Edward H. Frieden
Keyword(s):  
Biological Activity ◽  
Comparative Studies ◽  
Free Amino ◽  
Hormonal Activity ◽  
Amino Groups

ABSTRACT A number of corticotrophin analogues have been prepared, some of which potentiate the biological activity of the untreated hormone in vitro. The free amino groups of corticotrophin appear to be essential not only for hormonal activity, but also for the interaction of the analogues with the tissue corticotrophin inactivating system which is assumed to account for the potentiating effect.

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