Isolation and characterization of an UDPG-dependent glucosyltransferase activity from Rauwolfia serpentina Benth. cell suspension cultures
Keyword(s):
From cell suspension cultures of Rauwolfia serpentina Benth. a new enzyme activity was isolated and its properties determined. The enzyme is a soluble protein and catalyzes the transfer of a glucose moiety from UDPG to a wide variety of phenolic compounds with p-nitrophenol as one of the best substrates (Km = 1.21 mM, UDPG = 0.54 mM). In contrast to the membrane-bound UDPG: vomilenine-21-OH-β-D-glucosyltransferase from Rauwolfia serpentina cells, this enzyme is not able to glucosylate indole alkaloids. The enzyme activity has been detected in 14 callus cultures belonging to 10 different plant families.
1984 ◽
Vol 39
(6)
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pp. 525-530
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1990 ◽
Vol 45
(9-10)
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pp. 973-979
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Keyword(s):
2000 ◽
Vol 71
(1)
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pp. 84
1979 ◽
Vol 34
(3-4)
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pp. 200-209
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