rDNA internal transcribed spacer sequence analysis of Craterellus tubaeformis from North America and Europe

2011 ◽  
Vol 57 (1) ◽  
pp. 29-32
Author(s):  
Zhou Guo-ying ◽  
Guo Liang ◽  
Li Lin ◽  
Li He
Keyword(s):  
North America ◽  
Internal Transcribed Spacer ◽  
Information Sources ◽  
Geographical Location ◽  
Its Region ◽  
Molecular Evidence ◽  
Nrdna Its ◽  
Geographic Locations ◽  
Sequence Variations ◽  
Other Information

The basidiomycete Craterellus tubaeformis (Fries) Quélet is an important widespread ectomycorrhizal basidiomycete found in the Northern Hemisphere. In this study, 12 samples of C. tubaeformis from North America and Europe were analyzed using internal transcribed spacer (ITS) sequences to reveal the correlation between ITS genotypes and geographic locations and to provide molecular evidence for the identification of C. tubaeformis from different habitats in North America and Europe. The analyses identified abundant sequence variations within C. tubaeformis. The length of the ITS region varied from 571 to 640 bp. The proportion of variable sites was 17.6%, and the proportion of parsimony information sites was 16.7%. Phylogenetic analysis showed some correlations between the ITS genotypes and geographic locations of C. tubaeformis; however, some discrepancies between geographical location and affinity were also found. The results indicated that C. tubaeformis from different habitats in North America and Europe underwent genetic drifting and evolved into 2 different species. nrDNA ITS could be a good markers for distinguishing among C. tubaeformis from different habitats, but rational affinity should be determined by associating the available ITS data with other information sources.

Morphological and molecular evidence for a new species of Russula (Russulaceae) from southern China

Phytotaxa ◽  
2015 ◽  
Vol 202 (2) ◽  
pp. 94 ◽  
Author(s):  
Yang Kun Li ◽  
Xin Zhang ◽  
Ye Yuan ◽  
Zheng Cao ◽  
Jun Feng Liang
Keyword(s):  
New Species ◽  
Related Species ◽  
Phylogenetic Relationships ◽  
Internal Transcribed Spacer ◽  
Southern China ◽  
Its Region ◽  
Molecular Evidence ◽  
Closely Related Species ◽  
A New Species

Russula subrutilans sp. nov., a new species of Russula is described from southern China. It is unique for having buff pink to light congo-pink pileus, distant ventricose to subventricose lamellae with rare lamellulae, globose to broadly ellipsoid spores with bluntly conical warts forming a partial reticulum, and narrowly clavate to clavate cheilocystidia and pleurocysitidia with variable tips. Phylogenetic relationships among the new species and other closely related species in the genus are inferred based on the internal transcribed spacer (ITS) region.

Pilea nonggangensis (Urticaceae), a new species from Guangxi, China

Phytotaxa ◽  
2017 ◽  
Vol 313 (1) ◽  
pp. 130 ◽  
Author(s):  
LONG-FEI FU ◽  
SHI-LIAN HUANG ◽  
ALEX K. MONRO ◽  
YING LIU ◽  
FANG WEN ◽  
...  
Keyword(s):  
New Species ◽  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Species Conservation ◽  
Its Region ◽  
Systematic Position ◽  
Molecular Evidence ◽  
Conservation Assessment ◽  
Dna Sequence Data ◽  
A New Species

Pilea nonggangensis Y. G. Wei, L. F. Fu & A. K. Monro, a new species from Guangxi, China is described and illustrated. The new species is morphologically most similar to P. basicordata from which it differs by being dioecious, having peltate leaf blades, membranous stipules, paired inflorescences and larger bracts. Nuclear ribosomal internal transcribed spacer (ITS) region and plastid trnL-F intron spacer (trnL-F) DNA sequence data from the new species and 20 local congeneric species are used to resolve the systematic position of the new species within Pilea. Despite vastly different morphology the molecular evidence suggests that P. nonggangensis is most closely related to P. pseudonotata. A Global Species Conservation Assessment classifies P. nonggangensis as Vulnerable (VU).

Hygrocybe umbilicata sp. nov., with first generic report for Bangladesh and its phylogenetic placement

Phytotaxa ◽  
2016 ◽  
Vol 280 (1) ◽  
pp. 70 ◽  
Author(s):  
MD. IQBAL HOSEN ◽  
TAI-HUI LI ◽  
F. M. AMINUZZAMAN ◽  
MD. RAFIQUL ISLAM
Keyword(s):  
New Species ◽  
Internal Transcribed Spacer ◽  
Its Region ◽  
First Record ◽  
Morphological Data ◽  
Lemon Yellow ◽  
Molecular Evidence ◽  
Phylogenetic Placement

Hygrocybe umbilicata, a new waxcap of Hygrophoraceae, is formally described from Bangladesh based on morphological data and molecular evidence of the nuclear ribosomal internal transcribed spacer (ITS) region. This species represents the first record of the genus Hygrocybe for this country. It can be easily recognized by its brilliantly scarlet red with umbilicate pileus, lemon yellow stipe, white short decurrent and distant lamellae, subglobose basidiospores, and abundant clamp connections in tissues. This species belongs to genus Hygrocybe subg. Pseudohygrocybe sect. Coccineae subsect. Squamulosae. Detailed description, color photographs and illustrations of the new species are presented.

scholarly journals Michaelmoelleria (Gesneriaceae), a new lithophilous dwelling genus and species with zigzag corolla tube from southern Vietnam

PhytoKeys ◽  
2020 ◽  
Vol 146 ◽  
pp. 89-107
Author(s):  
Fang Wen ◽  
Zi-Bing Xin ◽  
Long-Fei Fu ◽  
Shu Li ◽  
Lan-Ying Su ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
New Genus ◽  
Sequence Data ◽  
Its Region ◽  
Corolla Tube ◽  
Single Species ◽  
Molecular Evidence ◽  
Conservation Assessment ◽  
Dna Sequence Data ◽  
Southern Vietnam

Michaelmoelleria, a new genus from southern Vietnam is described with a single species, M. vietnamensis. The new genus is morphologically most similar to Deinostigma and Tribounia but it differs from the latter two by having four fertile stamens. Nuclear ribosomal internal transcribed spacer (ITS) region and plastid trnL-F intron spacer (trnL-F) DNA sequence data from the new genus and eighty-seven species representing 42 genera within tribe Didymocarpeae are used to resolve its generic placement. The molecular evidence reveals that it is most closely related to Cathayanthe rather than Deinostigma and Tribounia. The chromosome number is counted as 2n = 36 that further clarified its distinction comparing to the related genera within tribe Didymocarpeae. A global conservation assessment is also performed and classifies Michaelmoelleria vietnamensis as Critically Endangered (CR).

Lost and found: the Bermudan Donadinia seaveri found in North America, with comments on its juniper associates

Mycologia ◽  
2018 ◽  
Vol 110 (1) ◽  
pp. 215-221
Author(s):  
Pfister ◽  
LoBuglio
Keyword(s):  
North America ◽  
Internal Transcribed Spacer ◽  
Its Region ◽  
28S Rdna ◽  
Eastern North America ◽  
Lost And Found ◽  
Insect Attack

Collections of a species referred to Sarcosomataceae (Pezizomycetes) from eastern North America were studied both morphologically and using nuc rDNA internal transcribed spacer (ITS) region (ITS1-5.8S-ITS2 = ITS) and approximately 800 bp from the 5′ region of the nuc 28S rDNA (28S) to construct a phylogeny. The analyses indicate that these collections are Donadinia seaveri, a species previously known only from Bermuda. Because the associated tree, Juniperus bermudiana, has declined as a result of insect attack, it was thought that D. seaveri might be extinct. This work indicates that it is not extinct but is present in eastern North America. The species is described, new distributional records are given, and its association with the genus Juniperus is discussed.

scholarly journals Morphological and molecular identification of four new resupinate species of Lyomyces (Hymenochaetales) from southern China

MycoKeys ◽  
2020 ◽  
Vol 65 ◽  
pp. 101-118
Author(s):  
Jun-Zhu Chen ◽  
Chang-Lin Zhao
Keyword(s):  
New Species ◽  
Molecular Identification ◽  
Internal Transcribed Spacer ◽  
Southern China ◽  
Phylogenetic Analyses ◽  
Its Region ◽  
Molecular Data ◽  
Fungal Species ◽  
Molecular Evidence ◽  
Thin Walled

Four new wood-inhabiting fungal species, Lyomyces bambusinus, L. cremeus, L. macrosporus and L. wuliangshanensis, are proposed based on a combination of morphological and molecular evidence. Lyomyces bambusinus is characterized by resupinate basidiomata with colliculose to tuberculate hymenial surface and broadly ellipsoid, hyaline, slightly thick-walled, smooth basidiospores. Lyomyces cremeus is characterised by resupinate basidiomata with smooth, cream hymenial surface and ellipsoid, hyaline, thin-walled to slightly thick-walled basidiospores. Lyomyces macrosporus is characterized by pruinose basidiomata with reticulate hymenial surface, presence of three kinds of cystidia and larger basidiospores (6.7–8.9 × 4.4–5.4 µm). Lyomyces wuliangshanensis is characterized by coriaceous basidiomata and ellipsoid, hyaline, slightly thick-walled, smooth basidiospores. The phylogenetic analyses based on molecular data of the internal transcribed spacer (ITS) region sequences revealed that the four new species belonged to Lyomyces. Lyomyces bambusinus grouped with L. sambuci. Lyomyces cremeus clade was sister to a clade comprised of L. microfasciculatus. Lyomyces macrosporus was sister to L. allantosporus. Lyomyces wuliangshanensis was closely related to L. mascarensis.

Russula fanjing, a new species of Russula subsect. Russula (Russulaceae, Russulales) from Guizhou province, China

Phytotaxa ◽  
2021 ◽  
Vol 480 (2) ◽  
pp. 139-151
Author(s):  
FANG LI ◽  
GUO-JIE LI ◽  
JING ZHANG ◽  
HUAN GAO ◽  
RU-SHU SHI ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Related Species ◽  
Internal Transcribed Spacer ◽  
Its Region ◽  
Guizhou Province ◽  
Molecular Evidence ◽  
New Taxon ◽  
Line Drawings ◽  
A New Species

Russula fanjing is proposed here as a new species based on morphological and molecular evidence. The new species is described and illustrated with photographs and line drawings and compared to related species. Morphologically, R. fanjing (subgenus Russula, section Russula, subsection Russula) is characterized by a medium-sized basidioma, with a vivid red to pastel red areolate pileus, white lamellae occasionally forked near the stipe with lamellulae, a smooth white stipe, basidiospores ornamented with strongly amyloid warts and ridges interconnected by fine lines in an incomplete or complete reticulum, and a pileipellis a typical trichoderm with suprapellis cells composed of short-celled, claw-like branched, lotus-root-like inflated hyphae and dispersed clavate, septate pileocystidia. The phylogenetic analysis of the nucleotide sequences of the internal transcribed spacer (ITS) region provided further evidence that the described species belongs to subsection Russula and represents a new taxon.

Relationships among problematic North American stagnicoline snails (Pulmonata: Lymnaeidae) reinvestigated using nuclear ribosomal DNA internal transcribed spacer sequences

1997 ◽  
Vol 75 (9) ◽  
pp. 1540-1545 ◽  
Author(s):  
E. A. Remigio ◽  
D. Blair
Keyword(s):  
North America ◽  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Its Region ◽  
Nucleotide Sequences ◽  
Its Sequences ◽  
Nuclear Ribosomal Dna ◽  
Snail Species ◽  
Separate Species ◽  
Internal Transcribed Spacer Sequences

The taxonomic relationships among four stagnicoline snail species from North America were reassessed by analysing nucleotide sequences from their nuclear ribosomal internal transcribed spacer (ITS) region. Three of the species (Stagnicola catascopium, Stagnicola emarginata, and Stagnicola elodes) are representatives of the subgenus Stagnicola s.str. from the northern U.S.A. and the fourth is a closely related Canadian species (Stagnicola caperata) belonging to the subgenus Hinkleyia. Members of the two subgenera differed considerably in their ITS sequences, demonstrating the usefulness of these sequences for distinguishing species from closely related lymnaeid subgenera. In contrast, ITS sequences for the three members of the subgenus Stagnicola s.str. were very similar, which supports an earlier view that they may not be separate species.

Molecular evidence for reticulate speciation in Astragalus (Fabaceae) as revealed by a case study from sect. Dissitiflori

Botany ◽  
2013 ◽  
Vol 91 (10) ◽  
pp. 702-714 ◽  
Author(s):  
László Bartha ◽  
Nicolae Dragoş ◽  
Attila Molnár V. ◽  
Gábor Sramkó
Keyword(s):  
Incomplete Lineage Sorting ◽  
Phylogenetic Network ◽  
Evolutionary Process ◽  
Its Region ◽  
Molecular Evidence ◽  
Nrdna Its ◽  
Lineage Sorting ◽  
Ancestral Polymorphism ◽  
Shed Light

Although hybridization has long been recognized as a major force driving speciation in land plants, it has not yet been evidenced in Astragalus, the largest angiosperm genus. Here, we reveal the possible contribution of hybridization to speciation in Astragalus by employing cloning of the nrDNA ITS region and sampling three plastid regions (ycf1, ndhF–rpl32, and rpl32–trnL) in taxa belonging to sect. Dissitiflori. Phylogenetic network and tree analyses uncovered various levels of intra-individual and intraspecific polymorphism of ITS in most of the taxa investigated. Two distantly related ribotype groups were found to be shared by the closely related polyploids Astragalus pallescens M.Bieb., Astragalus peterfii Jáv., and Astragalus pseudoglaucus Klokov suggesting ancient hybridization followed by incomplete lineage sorting (i.e., shared ancestral polymorphism) in nrDNA ITS. Reticulation is also invoked as an underlying evolutionary process behind the statistically highly supported incongruent placement of A. pseudoglaucus and Astragalus vesicarius subsp. pastellianus (Pollini) Arcang. in nuclear versus plastid phylogenies. The phylogenetic results also shed light on taxonomic controversies in the section, such as the false synonimization of A. peterfii under A. vesicarius s.l. Our results provide evidence for the (at least past) existence of speciation processes driven by hybridization in Astragalus.

RAILROAD INVESTING AND THE IMPORTANCE OF FINANCIAL ACCOUNTING INFORMATION IN 1880s AMERICA

2013 ◽  
Vol 40 (2) ◽  
pp. 55-89 ◽  
Author(s):  
Joel E. Thompson
Keyword(s):  
United States ◽  
Information Sources ◽  
Accounting Information ◽  
The United States ◽  
Financial Accounting ◽  
Wall Street ◽  
New Information ◽  
Other Information ◽  
Comprehensive Search ◽  
The Wall Street Journal

This study has a two-fold purpose. First, it seeks to determine the importance of financial accounting information to railroad investors (and speculators) in 1880s America. Second, a further goal is to ascertain what financial accounting information was readily available for use by these investors. Based on a comprehensive search of books of the era, the 1880s were a time of expanding advice for railroad securities holders that required the use of financial accounting information. Furthermore, new information sources arose to help service investors' needs. Statistics by Goodsell and The Wall Street Journal were two such sources. This article reviews these publications along with the ongoing Commercial and Financial Chronicle and Poor's Manual of the Railroads of the United States. Each of these sources helped railroad investors to follow contemporary advice of gathering financial accounting and other information when investing.

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