Characterization of an endophytic whorl-forming Streptomyces from Catharanthus roseus stems producing polyene macrolide antibiotic

2012 ◽  
Vol 58 (5) ◽  
pp. 617-627 ◽  
Author(s):  
Erick Francisco Rakotoniriana ◽  
Gabrielle Chataigné ◽  
Guy Raoelison ◽  
Christian Rabemanantsoa ◽  
Françoise Munaut ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Catharanthus Roseus ◽  
Macrolide Antibiotic ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Cryphonectria Parasitica ◽  
Streptomyces Sp ◽  
Polyene Macrolide Antibiotic ◽  
Layer Chromatography

An endophytic whorl-forming Streptomyces sp. designated as TS3RO having antifungal activity against a large number of fungal pathogens, including Sclerotinia sclerotiorum , Rhizoctonia solani , Colletotrichum gloeosporioides , Cryphonectria parasitica , Fusarium oxysporum , Pyrenophora tritici-repentis , Epidermophyton floccosum , and Trichophyton rubrum , was isolated from surface-sterilized Catharanthus roseus stems. Preliminary identification showed that Streptomyces cinnamoneus subsp. sparsus was its closest related species. However, strain TS3RO could readily be distinguished from this species using a combination of phenotypic properties, 16S rDNA sequence similarity, and phylogenetic analyses. Thus, the whorl-forming Streptomyces sp. strain TS3RO is likely a new subspecies within the Streptomyces cinnamoneus group. Direct bioautography on a thin-layer chromatography plate with Cladosporium cucumerinum was conducted throughout the purification steps for bioassay-guided isolation of the active antifungal compounds from the crude extract. Structural elucidation of the isolated bioactive compound was obtained via LC–MS spectrometry, UV-visible spectra, and nuclear magnetic resonance data. It revealed that fungichromin, a known methylpentaene macrolide antibiotic, was the main antifungal component of TS3RO strain, as shown by thin-layer chromatography bioautography. This is the first report of an endophytic whorl-forming Streptomyces isolated from the medically important plant Catharanthus roseus.

scholarly journals PENGARUH MANIPULASI MEDIA TERHADAP KANDUNGAN ALKALOID VINKRISTINA KALUS DAUN Catharanthus roseus(L.) G.Don

1995 ◽  
Vol 1 (1) ◽  
pp. 1-7
Author(s):  
Y. Sri Wulan Manuhara
Keyword(s):  
Tissue Culture ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Catharanthus Roseus ◽  
Leaf Explants ◽  
Preparative Thin Layer Chromatography ◽  
Uv Spectra ◽  
Basic Medium ◽  
The Media ◽  
Layer Chromatography

The effect of the manipulation of the media on the vincristine alkaloid content in the callus of Catharanthus roseus (L.) G.Don were studied. This work was done as an effort for gaining the vincristine alkaloid through tissue culture which was expected to obtain a larger amount of the alkaloid. Tissue culture of C. roseus was initiated from leaf explants on growth medium (MS supplemented with 1 mg per l 2,4-D and 1 mg per l BAP). after seven weeks incubation, with only subculturing on the same medium, the proliferating calli were subculture on a production medium (MS supplemented with 1mg per l IAA and 1 mg per l BAP) which were containing different additional concentration of sucrose, BAP, tryptophan, and concentration of 50 percent basic medium of MS from the standard. Eleven weeks-old calli were harvested from each treatment and dried for chemical analysed by thin layer chromatography on silica gel GF 254 using chloroform-aceton-triethylamine as mobile phase. Rf value and uv spectra were used to identify vincristine, and concentration of vincristine alkaloid was determine by preparative thin layer chromatography with methanol solvent and measured by uv-vis spectrophotometer at 233 nm. The maximum content of vincristine alkaloid was obtained from callus, which was grown on the MS standard with an addition of 40 g per l sucrose or 4 mg per l BAP or and also 50 percent of the MS standard medium. Tryptophan addition a precursor could not induce the alkaloid vincristine forming.

The Phosphatidylinositol Species of Suspension Cultured Plant Cells

1987 ◽  
Vol 42 (7-8) ◽  
pp. 1003-1005 ◽  
Author(s):  
Sabina Heim ◽  
Karl G. Wagner
Keyword(s):  
Nicotiana Tabacum ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Catharanthus Roseus ◽  
Plant Cells ◽  
Phospholipid Fraction ◽  
Reference Compound ◽  
Faint Band ◽  
Solvent Systems ◽  
Layer Chromatography

Suspension cultured Nicotiana tabacum and Catharanthus roseus cells were labeled with [3H]inositol, the phospholipid fraction extracted and separated by thin layer chromatography. Three different solvent systems and reference compounds were used to assign the different 3H-labeled species by autoradiography. The ratio of [3H]inositol incorporation into PI, PIP and PIP2 was found to be 95:4:1; with some preparations a lyso-PI band was obtained which incorporated about a tenth of the label of the PIP band. With Catharanthus roseus cells a very faint band between PI and lyso-PI was detected which could not be assigned to a reference compound.

Identification of chemotypesin Holy Basil (Ocimum sanctum) by High Performance Thin Layer Chromatography (HPTLC)

Planta Medica ◽  
2014 ◽  
Vol 80 (16) ◽  
Author(s):  
T Schinle ◽  
I Merfort ◽  
D Frommenwiler ◽  
E Reich
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Ocimum Sanctum ◽  
Layer Chromatography ◽  
Holy Basil

Thin-Layer Chromatography

1965 ◽  
Author(s):  
H. R. Bolliger ◽  
M. Brenner ◽  
H. Gänshirt ◽  
Helmut K. Mangold ◽  
H. Seiler ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Layer Chromatography

THE LESS-POLAR METABOLITES PRODUCED BY INCUBATION OF TESTOSTERONE-4-14C WITH RAT AND BOVINE BRAIN

1969 ◽  
Vol 61 (4) ◽  
pp. 641-648 ◽  
Author(s):  
Leon J. Sholiton ◽  
Emile E. Werk
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Paper Chromatography ◽  
Specific Activity ◽  
Bovine Brain ◽  
Polar Fraction ◽  
End Products ◽  
Polar Metabolites ◽  
Layer Chromatography ◽  
Constant Specific Activity

ABSTRACT Rat and bovine brain have been incubated with testosterone-4-14C under standard conditions. With use of paper chromatography, the extracted metabolites were noted to fall into less-polar, iso-polar, and more polar fractions. The components of the less-polar fraction were separated by acetylation and thin-layer chromatography and the major end-products identified by recrystallization to constant specific activity or constant 3H/14C ratios. Androst-4-enedione and 5α-dihydrotestosterone were formed consistently under the conditions utilized. Trace amounts of other less-polar metabolites were noted occasionally.

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