THE LESS-POLAR METABOLITES PRODUCED BY INCUBATION OF TESTOSTERONE-4-14C WITH RAT AND BOVINE BRAIN

1969 ◽  
Vol 61 (4) ◽  
pp. 641-648 ◽  
Author(s):  
Leon J. Sholiton ◽  
Emile E. Werk
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Paper Chromatography ◽  
Specific Activity ◽  
Bovine Brain ◽  
Polar Fraction ◽  
End Products ◽  
Polar Metabolites ◽  
Layer Chromatography ◽  
Constant Specific Activity

ABSTRACT Rat and bovine brain have been incubated with testosterone-4-14C under standard conditions. With use of paper chromatography, the extracted metabolites were noted to fall into less-polar, iso-polar, and more polar fractions. The components of the less-polar fraction were separated by acetylation and thin-layer chromatography and the major end-products identified by recrystallization to constant specific activity or constant 3H/14C ratios. Androst-4-enedione and 5α-dihydrotestosterone were formed consistently under the conditions utilized. Trace amounts of other less-polar metabolites were noted occasionally.

THE ISO-POLAR METABOLITES PRODUCED BY INCUBATION OF [4-14C]TESTOSTERONE WITH RAT AND BOVINE BRAIN

1970 ◽  
Vol 63 (3) ◽  
pp. 512-518 ◽  
Author(s):  
Leon J. Sholiton ◽  
Ila L. Hall ◽  
Emile E. Werk
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Rat Brain ◽  
Specific Activity ◽  
Bovine Brain ◽  
Polar Fraction ◽  
Gas Liquid Chromatography ◽  
Polar Metabolite ◽  
Polar Metabolites ◽  
Layer Chromatography

ABSTRACT Rat and bovine brain homogenates have been incubated with [4-14C] testosterone under standard conditions. With use of paper chromatography, the extracted metabolites were noted to fall into less polar, iso-polar, and more polar fractions. The components of the iso-polar fraction were separated after acetylation by thin-layer chromatography and the major end-product identified by gas-liquid chromatography and recrystallization to constant specific activity. Rat brain forms a major iso-polar metabolite, allo-tetrahydrotestosterone, and a small amount of tetrahydrotestosterone. Neither of these could be detected in the bovine brain incubates.

PATHWAYS IN THE BIOSYNTHESIS OF ANDROGENS IN THE POSTMENOPAUSAL OVARY IN VITRO

1971 ◽  
Vol 66 (2) ◽  
pp. 325-332 ◽  
Author(s):  
J. G. Schenker ◽  
W. Z. Polishuk ◽  
B. Eckstein
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Specific Activity ◽  
Ovarian Tissue ◽  
Tissue Homogenates ◽  
Layer Chromatography ◽  
Constant Specific Activity

ABSTRACT Postmenopausal ovarian tissue homogenates were incubated with [7α-3H]-pregnenolone as substrate. In six independent incubations only androstenedione and testosterone were found. These androgens were isolated by column and thin-layer chromatography and identified by derivative formation and recrystallization to constant specific activity. In one of the incubations, the homogenate was divided into 3 parts. From one part incubated with [7α-3H] pregnenolone, androstenedione and testosterone were identified. From the second and third parts of the homogenate which were incubated with [4-14C]progesterone and [4-14C] testosterone respectively, the substrates were recovered unmetabolized at the end of the incubation. From these results it is deduced that the postmenopausal ovary can not aromatize androgens to oestrogens and that in this particular tissue the Δ5 pathway is the preferred route of androstenedione production.

FREE AMINO ACIDS IN THE HAEMOLYMPH OF RHODNIUS PROLIXUS STÅHL (HEMIPTERA: REDUVIIDAE)

1966 ◽  
Vol 44 (2) ◽  
pp. 241-249 ◽  
Author(s):  
Catharine Pickett ◽  
W. G. Friend
Keyword(s):  
Amino Acids ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Paper Chromatography ◽  
Free Amino Acids ◽  
Free Amino ◽  
Rhodnius Prolixus ◽  
Layer Chromatography

Nineteen free amino acids and four ninhydrin-positive compounds were detected in the haemolymph of fifth-instar nymphs of Rhodnius prolixus by the technique of thin-layer chromatography. On the sixth day after feeding, tyrosine and tryptophan appeared in the haemolymph. Their concentration increased until the 12th day when testing stopped. The concentrations of alanine, asparagine, cystine, isoleucine, phenylalanine, and threonine fluctuated moderately from day to day. The results obtained by thin-layer chromatography differed from those of a similar study in which the technique of paper chromatography was used.

The polar lipids and free sugars of Frankia in culture

1983 ◽  
Vol 61 (11) ◽  
pp. 2834-2842 ◽  
Author(s):  
Mary F. Lopez ◽  
Carol S. Whaling ◽  
John G. Torrey
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Paper Chromatography ◽  
Phosphatidyl Inositol ◽  
Free Sugars ◽  
Whole Cells ◽  
Cyanobacterium Anabaena ◽  
The Media ◽  
Layer Chromatography ◽  
Chloroform Methanol

To further the identification of characteristics common to Frankia strains, the phospholipids and sugars which are readily extracted with chloroform–methanol from whole cells were examined using nine strains of Frankia. Separation of extract components was achieved by thin-layer chromatography. In agreement with previous reports we have identified two phospholipids, phosphatidyl inositol and diphosphatidyl glycerol, in eight of the strains tested. All strains contained a glucose disaccharide which we have tentatively identified as trehalose on the basis of (i) comparative thin-layer and paper chromatography with standards, (ii) examination of the hydrolyzed product, and (iii) tests for reducing versus nonreducing sugars. In addition, a hexose with the chromatographic properties of glucose was observed in cultures grown on M6B complex medium. The presence of the phospholipids in Frankia strains was not affected by the age of the culture or the media on which they grew. However, the disaccharide was not present in cultures over 60 days of age, suggesting it is involved in metabolic processes. In addition, comparisons were made between extracts from Frankia and the nitrogen-fixing cyanobacterium Anabaena variabilis to determine if the glycolipids which are unique to Anabaena heterocysts are present in Frankia. These two organisms appear to have an unidentified lipid in common, but glycolipids comparable with those in Anabaena were not found in the Frankia cultures examined.

Movement and Activity of Glyphosate in Purple Nutsedge

Weed Science ◽  
1977 ◽  
Vol 25 (3) ◽  
pp. 268-274 ◽  
Author(s):  
Bernard H. Zandstra ◽  
Roy K. Nishimoto
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Specific Activity ◽  
Cyperus Rotundus ◽  
Plant Age ◽  
Purple Nutsedge ◽  
Plant Parts ◽  
Layer Chromatography ◽  
Application Specific

Tubers from purple nutsedge (Cyperus rotundusL.) plants grown in the greenhouse for 2 to 10 weeks did not germinate after foliar applications of 4 kg/ha glyphosate (N-(phosphonomethyl)glycine). Some tubers from 12- and 24-week-old purple nutsedge plants survived glyphosate application. Translocation of14C-glyphosate from treated purple nutsedge leaves to other plant parts increased from 5% of the amount applied at 1 day to 19% at 4 days after application. Specific activity of14C in tubers was greater than in leaves of plants 2- to 6-week-old. With increasing plant age, specific activity decreased in both tubers and leaves. As purple nutsedge grew older, total14C translocated increased in tubers, and decreased slightly in leaves. Thin layer chromatography showed no evidence of glyphosate metabolism in purple nutsedge.

Determination of specific activity of isotopic materials by thin layer chromatography

Lipids ◽  
1972 ◽  
Vol 7 (6) ◽  
pp. 420-425 ◽  
Author(s):  
Wil Wortmann ◽  
Maria Kasparow ◽  
Joseph C. Touchstone
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Specific Activity ◽  
Layer Chromatography
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