Cultural and PCR-based identification of the two most common fungi from cankers on container-grown Norway spruce seedlings

2005 ◽  
Vol 35 (2) ◽  
pp. 432-439 ◽  
Author(s):  
A Lilja ◽  
M Poteri ◽  
M Vuorinen ◽  
T Kurkela ◽  
J Hantula
Keyword(s):  
Norway Spruce ◽  
18S Rdna ◽  
Single Species ◽  
Microsatellite Analysis ◽  
Malt Extract ◽  
Operational Taxonomic Units ◽  
Phoma Herbarum ◽  
Colony Color ◽  
18S Rdna Sequence ◽  
Related Fungus

Assays were made to identify the most common fungi in cankers occurring on container seedlings of Norway spruce. The fungi isolated from cankers were first separated into morphological groups based on colony color, growth rate, and pattern on malt extract agar. Profiling of 18S rDNA indicated that several operational taxonomic units (OTUs) inhabited the cankers, some (OTU1 and OTU2) of which could be linked to mycelial cultures also isolated from the cankers. In addition, OTU1 could be linked to isolates originating from damped-off seedlings. Although the cultures linked to OTU1 differed morphologically from each other, the random amplified microsatellite analysis showed that they were genetically highly similar and represented a single species. Later, when these cultures were kept in natural sunlight, they all produced conidia morphologically similar to Sirococcus conigenus. Also, their partial 18S rDNA sequence was identical to that of S. conigenus in GenBank. The other common fungus (OTU2) was not possible to identify to species level, but based on comparisons with sequences in GenBank, it was closely related to Phoma herbarum. In preliminary tests, both S. conigenus (OTU1) and P. herbarum related fungus (OTU2) were pathogenic to Norway spruce seedlings.

Phylogenetic analyses of various euglenoid taxa (euglenozoa) based on 18s rdna sequence data

2000 ◽  
Vol 36 (1) ◽  
pp. 220-226 ◽  
Author(s):  
Angelika Preisfeld ◽  
Silke Berger ◽  
Ingo Busse ◽  
Susanne Liller ◽  
Hans Georg Ruppel
Keyword(s):  
18S Rdna ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Rdna Sequence ◽  
Rdna Sequence Data ◽  
18S Rdna Sequence

Development of a PCR strategy for thraustochytrid identification based on 18S rDNA sequence

2002 ◽  
Vol 140 (5) ◽  
pp. 883-889 ◽  
Author(s):  
Caiqing Mo ◽  
Douek J. ◽  
Rinkevich B.
Keyword(s):  
18S Rdna ◽  
Rdna Sequence ◽  
18S Rdna Sequence

scholarly journals Cytological And Molecular Identification Of Haemogregarina Stepanowi In Blood Samples Of The European Pond Turtle (Emys Orbicularis) From Quarantine At Belgrade Zoo

2015 ◽  
Vol 65 (4) ◽  
pp. 443-453 ◽  
Author(s):  
Jòzsef Özvegy ◽  
Darko Marinković ◽  
Miloš Vučićević ◽  
Bojan Gajić ◽  
Jevrosima Stevanović ◽  
...  
Keyword(s):  
Molecular Identification ◽  
18S Rdna ◽  
Clinical Symptoms ◽  
Health Condition ◽  
Rdna Sequence ◽  
Future Research ◽  
Emys Orbicularis ◽  
Direct Dna Sequencing ◽  
Hematological Analysis ◽  
18S Rdna Sequence

Abstract Blood smears stained with Diff Quick are the initial tool for cytological diagnosis of Haemogregarina spp. However, the development of sensitive and specific molecular methods enabled the detection and identification of parasites in the sample and to clarify the evolutionary relationships of adeleorinid parasites within the Apicomplexa. The current study was attempted in order to perform cytological investigation and molecular identification of the hemoparasites in thirty European pond turtles (Emys orbicularis) from the quarantine section at Belgrade Zoo, which have been found in poor health condition with massive skin hemorrhages, based on intraerythrocytic parasitic forms on hematological smears and 18S rDNA sequence, respectively. Different life cycle stages of the Haemogregarina sp. were noticed within the erythrocytes in the peripheral blood. Biochemical analysis indicated lower values of AST and iron in most of the infected turtles while hematological analysis showed a changed hematocrit value, a decrease in the number of red blood cells and low hemoglobin levels. Amplifications of the 18S rDNA sequence of Haemogregarina were detected in 30/30 (100%) turtles with clinical symptoms. The identity of PCR products was confirmed by direct DNA sequencing. Future research concerning H. stepanowi in Serbia should be applied to its definitive host-the leech.

scholarly journals Myxidium shedkoae Sokolov, 2013 (Myxozoa: Myxidiidae), a parasite of the gallbladder of Perccottus glenii Dybowski, 1877 (Actinoptrygii: Odontobutidae): Supplementary data on morphology and phylogenetic position based on 18s rDNA sequence analysis

2018 ◽  
Vol 66 (2) ◽  
pp. 258-268
Author(s):  
Sergey Sokolov ◽  
Daria Lebedeva
Keyword(s):  
Sequence Analysis ◽  
18S Rdna ◽  
Rdna Sequence ◽  
Phylogenetic Position ◽  
Amur Sleeper ◽  
Molecular Characterisation ◽  
Perccottus Glenii ◽  
Supplementary Data ◽  
18S Rdna Sequence ◽  
Partial 18S

This paper is the first report on the molecular characterisation of myxozoan parasites from the odontobutid fish Chinese (Amur) sleeper (Perccottus glenii Dybowski, 1877). The authors determined the partial 18S rDNA sequence of Myxidium shedkoae Sokolov, 2013 from the gallbladder of the fish. Phylogenies reconstructed using maximum likelihood and Bayesian inference analysis revealed that M. shedkoae belongs to the hepatic biliary group of myxozoans (after Kristmundsson and Freeman, 2013) as a member of the clade consisting of Zschokkella sp. KLT-2014, Myxidium truttae and Zschokkella nova. Some new morphological features of the parasite are also presented.

Chloroplast microsatellite analysis reveals the presence of population subdivision in Norway spruce (Picea abies K.)

Genome ◽  
2000 ◽  
Vol 43 (1) ◽  
pp. 68-78 ◽  
Author(s):  
G.G. Vendramin ◽  
M. Anzidei ◽  
A. Madaghiele ◽  
C. Sperisen ◽  
G. Bucci
Keyword(s):  
Picea Abies ◽  
Norway Spruce ◽  
Microsatellite Analysis ◽  
Population Subdivision ◽  
Chloroplast Microsatellite

Primary species recognition and phylogeny of Chondrus (Gigartinales, Rhodophyta) using 18S rDNA sequence data

2007 ◽  
Vol 25 (2) ◽  
pp. 174-183
Author(s):  
Zimin Hu ◽  
Xiaoqi Zena ◽  
Alan T. Critchley ◽  
Steve L. Morrell ◽  
Delin Duan
Keyword(s):  
18S Rdna ◽  
Sequence Data ◽  
Species Recognition ◽  
Rdna Sequence ◽  
Rdna Sequence Data ◽  
18S Rdna Sequence

Supplementary data of Henneguya leporinicola (Myxozoa, Myxosporea) a parasite of Leporinus macrocephalus from fish farms in the state of São Paulo, Brazil

2015 ◽  
Vol 60 (3) ◽  
Author(s):  
Kassia Roberta Hygino Capodifoglio ◽  
Edson Aparecido Adriano ◽  
Marcia Ramos Monteiro da Silva ◽  
Antônio Augusto Mendes Maia
Keyword(s):  
18S Rdna ◽  
Gill Filament ◽  
Morphological Characteristics ◽  
Sao Paulo ◽  
The State ◽  
Rdna Sequence ◽  
São Paulo ◽  
Fish Farms ◽  
Basal Branch ◽  
18S Rdna Sequence

AbstractHenneguya leporinicola is a parasite of the gill filament of Leporinus macrocephalus, a characiform fish belonging to the Anostomidae family, which is of major economic importance. Despite the damage it causes in fish, little is known about this parasite. Therefore, a study was undertaken with fourteen specimens of L. macrocephalus taken from fish farms in the state of Sao Paulo. The fish were collected and examined searching for lesions and/or myxosporean plasmodia. One of the specimens (7.14%) contained white elongated plasmodia in the gill filament. The mature spores had elongated bodies with polar capsules of equal size and a caudal length greater than body length. Morphological characteristics identified the parasite as H. leporinicola. Molecular analysis of the 18S rDNA sequence resulted in a 1954 bp, demonstrating significant genetic differences with previously described species of Henneguya/Myxobolus. Phylogenetic analysis comparing the 18S rDNA sequence of H. leporinicola with other species, previously described in South America, and the 20 closest species as indicated by BLASTn Max Score showed H. leporinicola as a basal branch of a subclade composed by Henneguya spp. parasite of characiform hosts.

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