SyntheticBacillus thuringiensis (Bt)toxin genes for insect resistance and their rapid bioassay using maize endosperm suspension cultures

2008 ◽  
pp. 737-742
Author(s):  
I. Altosaar ◽  
R. Sardana ◽  
S. Dukiandjiev ◽  
M. Giband ◽  
X. Cheng
Keyword(s):  
Insect Resistance ◽  
Suspension Cultures ◽  
Maize Endosperm ◽  
Bt Toxin ◽  
Toxin Genes ◽  
Rapid Bioassay

scholarly journals Exogenous Gene Expression and Insect Resistance in Dual Bt Toxin Populus × euramericana ‘Neva’ Transgenic Plants

2021 ◽  
Vol 12 ◽  
Author(s):  
Yachao Ren ◽  
Xinglu Zhou ◽  
Yan Dong ◽  
Jun Zhang ◽  
Jinmao Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Insect Resistance ◽  
Growth And Development ◽  
Hyphantria Cunea ◽  
Bt Toxin ◽  
Toxin Genes ◽  
Feeding Experiments ◽  
Expression Of Genes ◽  
Transgenic Lines ◽  
Exogenous Gene

Bacillus thuringiensis (Bt) insecticidal protein genes are important tools in efforts to develop insect resistance in poplar. In this study, the Cry1Ac and Cry3A Bt toxin genes were simultaneously transformed into the poplar variety Populus × euramericana ‘Neva’ by Agrobacterium-mediated transformation to explore the exogenous gene expression and insect resistance, and to examine the effects of Bt toxin on the growth and development of Anoplophora glabripennis larvae after feeding on the transgenic plant. Integration and expression of the transgenes were determined by molecular analyses and the insect resistance of transgenic lines was evaluated in feeding experiments. Sixteen transgenic dual Bt toxin genes Populus × euramericana ‘Neva’ lines were obtained. The dual Bt toxin genes were expressed at both the transcriptional and translational levels; however, Cry3A protein levels were much higher than those of Cry1Ac. Some of the transgenic lines exhibited high resistance to the first instar larvae of Hyphantria cunea and Micromelalopha troglodyta, and the first and second instar larvae and adults of Plagiodera versicolora. Six transgenic lines inhibited the growth and development of A. glabripennis larvae. The differences in the transcriptomes of A. glabripennis larvae fed transgenic lines or non-transgenic control by RNA-seq analyses were determined to reveal the mechanism by which Bt toxin regulates the growth and development of longicorn beetle larvae. The expression of genes related to Bt prototoxin activation, digestive enzymes, binding receptors, and detoxification and protective enzymes showed significant changes in A. glabripennis larvae fed Bt toxin, indicating that the larvae responded by regulating the expression of genes related to their growth and development. This study lay a theoretical foundation for developing resistance to A. glabripennis in poplar, and provide a foundation for exploring the mechanism of Bt toxin action on Cerambycidae insects.

Temporal and spatial changes in Bt toxin expression in Bt-transgenic poplar and insect resistance in field tests

2016 ◽  
Vol 27 (6) ◽  
pp. 1249-1256 ◽  
Author(s):  
Yiwen Zhang ◽  
Jun Zhang ◽  
Jinping Lan ◽  
Jinmao Wang ◽  
Junxia Liu ◽  
...  
Keyword(s):  
Insect Resistance ◽  
Field Tests ◽  
Transgenic Poplar ◽  
Bt Toxin ◽  
Spatial Changes ◽  
Temporal And Spatial

scholarly journals BtToxin_Digger: a comprehensive and high-throughput pipeline for mining toxin protein genes from Bacillus thuringiensis

2020 ◽  
Author(s):  
Hualin Liu ◽  
Jinshui Zheng ◽  
Dexin Bo ◽  
Yun Yu ◽  
Weixing Ye ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
High Throughput ◽  
Large Scale ◽  
Web Based ◽  
Bt Toxin ◽  
Link Type ◽  
Toxin Genes ◽  
Toxin Protein ◽  
Mining Tool ◽  
Downstream Analysis

SummaryBacillus thuringiensis (Bt) which is a spore-forming gram-positive bacterium, has been used as the most successful microbial pesticide for decades. Its toxin genes (cry) have been successfully used for the development of GM crops against pests. We have previously developed a web-based insecticidal gene mining tool BtToxin_scanner, which has been proved to be the most important method for mining cry genes from Bt genome sequences. To facilitate efficiently mining major toxin genes and novel virulence factors from large-scale Bt genomic data, we re-design this tool with a new workflow. Here we present BtToxin_Digger, a comprehensive, high-throughput, and easy-to-use Bt toxin mining tool. It runs fast and can get rich, accurate, and useful results for downstream analysis and experiment designs. Moreover, it can also be used to mine other targeting genes from large-scale genome and metagenome data with the addition of other query sequences.Availability and ImplementationThe BtToxin_Digger codes and instructions are freely available at https://github.com/BMBGenomics/BtToxin_Digger. A web server of BtToxin_Digger can be found at http://bcam.hzau.edu.cn/[email protected]; [email protected].

Isolation and sugar uptake characteristics of protoplasts from maize endosperm suspension cultures

1991 ◽  
Vol 81 (1) ◽  
pp. 83-88 ◽  
Author(s):  
Frederick C. Felker ◽  
Paul A. Thomas ◽  
C. Gerald Crawford
Keyword(s):  
Suspension Cultures ◽  
Sugar Uptake ◽  
Maize Endosperm

scholarly journals Sugar Uptake by Maize Endosperm Suspension Cultures

1988 ◽  
Vol 88 (4) ◽  
pp. 1235-1239 ◽  
Author(s):  
Frederick C. Felker ◽  
James C. Goodwin
Keyword(s):  
Suspension Cultures ◽  
Sugar Uptake ◽  
Maize Endosperm

Novel insect resistance in Brassica napus developed by transformation of chitinase and scorpion toxin genes

2005 ◽  
Vol 24 (9) ◽  
pp. 549-555 ◽  
Author(s):  
Jingxue Wang ◽  
Zhenlang Chen ◽  
Jianzhong Du ◽  
Yi Sun ◽  
Aihua Liang
Keyword(s):  
Brassica Napus ◽  
Insect Resistance ◽  
Scorpion Toxin ◽  
Toxin Genes

scholarly journals Genetic Markers for Western Corn Rootworm Resistance to Bt Toxin

2015 ◽  
Vol 5 (3) ◽  
pp. 399-405 ◽  
Author(s):  
Lex E Flagel ◽  
Shilpa Swarup ◽  
Mao Chen ◽  
Christopher Bauer ◽  
Humphrey Wanjugi ◽  
...  
Keyword(s):  
Genetic Markers ◽  
Insect Resistance ◽  
Management Strategies ◽  
Resistance Management ◽  
The United States ◽  
Western Corn Rootworm ◽  
Economic Losses ◽  
Corn Rootworm ◽  
Insect Resistance Management ◽  
Bt Toxin

Abstract Western corn rootworm (WCR) is a major maize (Zea mays L.) pest leading to annual economic losses of more than 1 billion dollars in the United States. Transgenic maize expressing insecticidal toxins derived from the bacterium Bacillus thuringiensis (Bt) are widely used for the management of WCR. However, cultivation of Bt-expressing maize places intense selection pressure on pest populations to evolve resistance. Instances of resistance to Bt toxins have been reported in WCR. Developing genetic markers for resistance will help in characterizing the extent of existing issues, predicting where future field failures may occur, improving insect resistance management strategies, and in designing and sustainably implementing forthcoming WCR control products. Here, we discover and validate genetic markers in WCR that are associated with resistance to the Cry3Bb1 Bt toxin. A field-derived WCR population known to be resistant to the Cry3Bb1 Bt toxin was used to generate a genetic map and to identify a genomic region associated with Cry3Bb1 resistance. Our results indicate that resistance is inherited in a nearly recessive manner and associated with a single autosomal linkage group. Markers tightly linked with resistance were validated using WCR populations collected from Cry3Bb1 maize fields showing significant WCR damage from across the US Corn Belt. Two markers were found to be correlated with both diet (R2 = 0.14) and plant (R2 = 0.23) bioassays for resistance. These results will assist in assessing resistance risk for different WCR populations, and can be used to improve insect resistance management strategies.

Sign in / Sign up

Export Citation Format

Share Document