In recent years, the use of liquid biofuels in the transport sector has shown rapid global growth, driven mostly by policies focused on achievement of energy security, and mitigation of GHG emissions. Microalgae, as biomass, are a potential source of renewable energy, and they can be converted into energy such as biofuel oil and gas.In this paper, the microalgae strain of Chlorella sp. was supplied in BG11 medium, and Cultivation optimization were experimented. The results were showed that temperature was an important factor which could influence growth and accumulation of secondary metabolite in the cell from accumulation process of biological products, temperature which was suitable for the growth of chlorella was 25°C.