Different Frequencies of Electroacupuncture Modified the Cellular Activity of Serotonergic Neurons in Brainstem

2000 ◽  
Vol 28 (03n04) ◽  
pp. 435-441 ◽  
Author(s):  
Young-bae Kwon ◽  
Myung-soo Kang ◽  
Sung-sae Son ◽  
Jun-tae Kim ◽  
Yon-ho Lee ◽  
...  

In this study, we evaluated whether different frequencies of electroacupuncture (EA) modified the activities of serotonergic neurons in the dorsal raphe (DR) and raphe magnus (RMg) using double labeling immunohistochemistry for Fos and serotonin. The results demonstrated that both high and low frequency EA increased the colocalization between Fos and serotonin in the DR, not in RMg as compared with anesthesia control. In addition, high frequency EA more potently increased the serotonergic activity in the DR rather than low frequency EA, suggesting that serotonergic path-way from the DR plays an important role in the high frequency EA analgesia.

2001 ◽  
Vol 888 (2) ◽  
pp. 321-325 ◽  
Author(s):  
Rubén Guzmán-Marı́n ◽  
Md.Noor Alam ◽  
Stefan Mihailescu ◽  
Ron Szymusiak ◽  
Dennis McGinty ◽  
...  

2020 ◽  
Vol 21 (8) ◽  
pp. 2735 ◽  
Author(s):  
Enaam Chleilat ◽  
Abhishek Pethe ◽  
Dietmar Pfeifer ◽  
Kerstin Krieglstein ◽  
Eleni Roussa

Calcium homeostasis is a cellular process required for proper cell function and survival, maintained by the coordinated action of several transporters, among them members of the Na+/Ca2+-exchanger family, such as SLC8A3. Transforming growth factor beta (TGF-β) signaling defines neuronal development and survival and may regulate the expression of channels and transporters. We investigated the regulation of SLC8A3 by TGF-β in a conditional knockout mouse with deletion of TGF-β signaling from Engrailed 1-expressing cells, i.e., in cells from the midbrain and rhombomere 1, and elucidated the underlying molecular mechanisms. The results show that SLC8A3 is significantly downregulated in developing dopaminergic and dorsal raphe serotonergic neurons in mutants and that low SLC8A3 abundance prevents the expression of the anti-apoptotic protein Bcl-xL. TGF-β signaling affects SLC8A3 via the canonical and p38 signaling pathway and may increase the binding of Smad4 to the Slc8a3 promoter. Expression of the lipid peroxidation marker malondialdehyde (MDA) was increased following knockdown of Slc8a3 expression in vitro. In neurons lacking TGF-β signaling, the number of MDA- and 4-hydroxynonenal (4-HNE)-positive cells was significantly increased, accompanied with increased cellular 4-HNE abundance. These results suggest that TGF-β contributes to the regulation of SLC8A3 expression in developing dopaminergic and dorsal raphe serotonergic neurons, thereby preventing oxidative stress.


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