MOLECULAR CHARACTERIZATION OF TWO NOVEL RAT MINUTE VIRUSES IN TAIWAN

2020 ◽  
Vol 46 (02n03) ◽  
pp. 101-110
Author(s):  
Yi-Chun Liao ◽  
Yung-Hui Chang ◽  
Ming-Hseng Wang ◽  
Ber-Hsiang Fang ◽  
Cho-Hua Wan
Keyword(s):  
Amino Acid ◽  
Molecular Characterization ◽  
Nucleotide Sequences ◽  
Amino Acid Sequences ◽  
Phylogenetic Data ◽  
Laboratory Rodent ◽  
Minute Virus ◽  
The Common ◽  
Kilham Rat Virus

Rodent parvovirus infection is one of the common viral problems in laboratory rodent colonies. In this study, two new parvoviruses were identified in naturally-infected rats from two different research colonies in Taiwan. The genomic nucleotide sequences and the predicted amino acid sequences of proteins for these two viruses were compared to the previously characterized rodent parvoviruses. The two newly identified viruses were most closely related to each other, also closely related to two variants of rat minute virus (RMV; RMV-1 and RMV-2), and distinct from but closely related to Kilham rat virus and H-1 virus. These two viruses were significantly different from variants of rat parvovirus (RPV; RPV-1 and RPV-NTU1). Phylogenetic data also supported that these two new viruses are variants of the RMV species. These two newly identified viruses were designated RMV type National Taiwan University 1 (RMV-NTU1) and RMV type National Taiwan University 2 (RMV-NTU2). RMV-NTUs are the first molecularly characterized RMV variants identified in Asia.

scholarly journals Molecular characterization of three newly recognized rat parvoviruses

2002 ◽  
Vol 83 (8) ◽  
pp. 2075-2083 ◽  
Author(s):  
Cho-Hua Wan ◽  
Maria Söderlund-Venermo ◽  
David J. Pintel ◽  
Lela K. Riley
Keyword(s):  
Amino Acid ◽  
Nucleotide Sequence ◽  
Molecular Characterization ◽  
Amino Acid Sequences ◽  
Minute Virus ◽  
Genomic Nucleotide Sequence ◽  
Kilham Rat Virus

Rodent parvoviruses have been documented to interfere with both in vivo and in vitro research. In this study, three rat parvoviruses distinct from previously characterized rodent parvoviruses were identified from naturally infected rats obtained from four discrete sources. These three newly recognized parvoviruses were designated rat minute virus (RMV)-1a, -1b and -1c. In this study, the genomic nucleotide sequence and the predicted amino acid sequences of proteins for each of the three RMV-1 variants and Kilham rat virus (KRV) were determined and compared with previously characterized rodent parvoviruses. The three RMV-1 variants were shown to be closely related to each other, to be distinct from but closely related to KRV and H-1 virus, and to be significantly different from the previously identified rat parvovirus isolate, RPV-1a.

scholarly journals Molecular characterization of Prunus necrotic ringspot virus isolated from rose in Brazil

2015 ◽  
Vol 45 (12) ◽  
pp. 2197-2200 ◽  
Author(s):  
Thor Vinícius Martins Fajardo ◽  
Monique Bezerra Nascimento ◽  
Marcelo Eiras ◽  
Osmar Nickel ◽  
Gilvan Pio-Ribeiro
Keyword(s):  
Amino Acid ◽  
Molecular Characterization ◽  
Molecular Analysis ◽  
Nucleotide Sequences ◽  
Amino Acid Sequences ◽  
Causal Agent ◽  
Ringspot Virus ◽  
Prunus Necrotic Ringspot Virus ◽  
First Time

ABSTRACT: There is no molecular characterization of Brazilian isolates of Prunus necrotic ringspot virus (PNRSV), except for those infecting peach. In this research, the causal agent of rose mosaic was determined and the movement (MP) and coat (CP) protein genes of a PNRSV isolate from rose were molecularly characterized for the first time in Brazil. The nucleotide and deduced amino acid sequences of MP and CP complete genes were aligned and compared with other isolates. Molecular analysis of the MP and CP nucleotide sequences of a Brazilian PNRSV isolate from rose and others from this same host showed highest identities of 96.7% and 98.6%, respectively, and Rose-Br isolate was classified in PV32 group.

scholarly journals Molecular Characterization of Chicken Anemia Virus Circulating in Chicken Flocks in Egypt

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Mohammed AboElkhair ◽  
Alaa G. Abd El-Razak ◽  
Abd Elnaby Y. Metwally
Keyword(s):  
Amino Acid ◽  
Molecular Characterization ◽  
Nucleotide Sequences ◽  
Amino Acid Sequences ◽  
Chicken Anemia Virus ◽  
Coding Region ◽  
Commercial Chicken ◽  
Amino Acids Profile ◽  
Anemia Virus

Introduction. Although many previous studies reported detection of chicken anemia virus (CAV) in Egypt since 1990, genomic characterization of this circulating CAV has not been published. In the present study, four nucleotide sequences of detected CAV were genetically characterized.Methods. These nucleotide sequences were obtained from commercial chicken flocks in two different locations of Egypt during 2010. The target region for sequencing was 675 bp nucleotide of partial coding region of VP1 protein. The nucleotide and deduced amino acid sequences of the detected CAV were aligned and compared to worldwide CAV isolates including commonly used vaccine strains. Phylogenetic analysis of these sequences was also carried out.Results. Our results showed that all the Egyptian CAV sequences were grouped in one group with viruses from diverse geographic regions. This group is characterized by amino acids profile75I,97L,139Q, and144Q in VP1. The phylogenetic and amino acid analyses of deduced amino acid indicated that the detected CAV sequences differ from CAV vaccine strains.Conclusion. This is the first report that describes molecular characterization of circulating CAV in Egypt. The study showed that the detected CAV, in Egypt are field viruses and unrelated to vaccine strains.

scholarly journals Molecular Characterization of Begomovirus on Cucumber in Java

2019 ◽  
Vol 23 (2) ◽  
pp. 197
Author(s):  
Listihani Listihani ◽  
Tri Asmira Damayanti ◽  
Sri Hendrastuti Hidayat ◽  
Suryo Wiyono
Keyword(s):  
Amino Acid ◽  
Molecular Characterization ◽  
Amino Acid Sequences ◽  
Specific Primer ◽  
West Java ◽  
Central Java ◽  
Frequency Detection ◽  
Squash Leaf Curl Virus ◽  
Leaf Curl

A survey on several cucumber cultivation areas in West Java, Central Java, Yogyakarta, and East Java found many plants showing typical Begomovirus symptoms such as yellow mosaic, cupping, and vein banding. This study was aimed to determine disease frequency, detection and molecular characterization of the causal virus of those symptoms on cucumber in Java. Sampling was conducted by purposive sampling by collecting 50 symptomatic plants from each location in West Java (Indramayu, Subang, and Bogor), Central Java (Brebes and Klaten), Yogyakarta (Kulon Progo), and East Java (Nganjuk, Kediri, and Tulungagung). The detection and disease frequency was determined based on DIBA test using a specific antiserum of Tomato leaf curl New Delhi virus (ToLCNDV) and Squash leaf curl virus (SLCV). The identification of nucleic acid was conducted by PCR using specific primer of ToLCNDV and SLCV, DNA cloning, and sequencing. The results of serological detection showed the disease frequency of ToLCNDV and SLCV ranged from 92.77-100% and 78.33-93.3%, respectively. PCR using specific primer of ToLCNDV successfully amplified the coat protein gene at a size of 600 bp from all samples. Homology nucleotide and amino acid sequences among ToLCNDV Java isolate ranging from 95.6-99.2% and 99.7-100%. ToLCNDV isolates Java had highest nucleotide and amino acid sequences similarity with cucumber isolate from Klaten, Indonesia (AB613825) ranging from 96.1-98.1% and 99.7-100%, and was considered as “Indonesia” strain. SLCV not amplified on all samples by PCR using specific primer, indicating it might not present yet on cucumber in Java.

Molecular characterization of ribonucleoproteic antigens containing repeated amino acid sequences from Trypanosoma cruzi

2008 ◽  
Vol 10 (7) ◽  
pp. 716-725 ◽  
Author(s):  
Fabiano S. Pais ◽  
Wanderson D. DaRocha ◽  
Ramon M. Almeida ◽  
Sophie Y. Leclercq ◽  
Marcus L. Penido ◽  
...  
Keyword(s):  
Amino Acid ◽  
Trypanosoma Cruzi ◽  
Molecular Characterization ◽  
Amino Acid Sequences

scholarly journals The genotype determination and molecular characterization of bovine leukemia virus in Turkey

2021 ◽  
Vol 91 (3) ◽  
pp. 237-247
Author(s):  
Feray Alkan ◽  
◽  
Ilke Karayel-Hacioglu ◽  
Selda Duran Yelken ◽  
Nuvit Coskun
Keyword(s):  
Amino Acid ◽  
Molecular Characterization ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Control Program ◽  
Amino Acid Sequences ◽  
Economic Losses ◽  
Geographical Regions ◽  
Full Length Sequence

Bovine leukemia virus (BLV) infection is widespread worldwide and causes significant economic losses in the livestock industry. In this study, to identify the genotypes and to investigate the molecular characterization of BLV strains, we analyzed 25 BLV strains from cattle housed in six herds located in several Turkish geographical regions. Phylogenetic analysis, based on a partial or full-length sequence of the env gp51 gene of BLVs, showed that all Turkish BLVs belonged to genotype 1. The alignment of the deduced amino acid sequences demonstrated fourteen amino acid substitutions in different regions of the env gp51. This study updates our knowledge of BLV in Turkey. Further studies are needed to understand the molecular epidemiology and implement a comprehensive BLV control program in Turkey.

scholarly journals Expression of Ubiquitin Gene in Microsporum canis and Trichophyton mentagrophytes Cultured with Fluconazole

2001 ◽  
Vol 45 (9) ◽  
pp. 2559-2562 ◽  
Author(s):  
Rui Kano ◽  
Ken Okabayashi ◽  
Yuka Nakamura ◽  
Shinichi Watanabe ◽  
Atsuhiko Hasegawa
Keyword(s):  
Amino Acid ◽  
Antifungal Activity ◽  
Proteasome Inhibitor ◽  
Trichophyton Mentagrophytes ◽  
Nucleotide Sequences ◽  
Amino Acid Sequences ◽  
Antifungal Drug ◽  
Microsporum Canis

ABSTRACT The expression of the ubiquitin (Ub) gene in dermatophytes was examined for its relation to resistance against the antifungal drug fluconazole. The nucleotide sequences and the deduced amino acid sequences of the Ub gene in Microsporum canis were proven to be 99% similar to those of the Ub gene in Trichophyton mentagrophytes. Expression of mRNA of Ub in M. canisand T. mentagrophytes was enhanced when the fungi were cultured with fluconazole. The antifungal activity of fluconazole against these dermatophytes was increased in the presence of Ub proteasome inhibitor.

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