A combined magnetophoresis/dielectrophoresis based microbead array as high-throughput biomolecular tweezers

In this paper we describe a combined magnetophoresis (MAP) and dielectrophoresis (DEP) based platform for high throughput characterization of specific biomolecular interactions. The magnetic manipulation enables parallel loading of individual magnetic beads onto a magnetic pad array, while the combination of tightly controlled opposing magnetic and dielectrophoretic (DEP) forces is employed to produce characteristic out-of-plane (z-axial) bead displacement. We optimized design parameters to evenly load 2.8 μm biomolecule functionalized paramagnetic beads onto magnetic pads, and demonstrate the ability of our tweezers to discriminate between specific antibody-antigen bond from non-specific bond formed between bead and pad surface.

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Combinatorial Fabrication and High-Throughput Characterization of Thin Film Metal Oxide Libraries for Solar water Splitting

10.29363/nanoge.fallmeeting.2018.046 ◽

2018 ◽

Author(s):

Alfred Ludwig ◽

Mona Nowak ◽

Swati Kumari ◽

Helge S. Stein ◽

Ramona Gutkowski ◽

...

Keyword(s):

Thin Film ◽

Metal Oxide ◽

Water Splitting ◽

High Throughput ◽

Solar Water ◽

Solar Water Splitting

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Faculty Opinions recommendation of Characterization of specific antibody deficiency in adults with medically refractory chronic rhinosinusitis.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.12742957.14012055 ◽

2011 ◽

Author(s):

Todd A Loehrl

Keyword(s):

Chronic Rhinosinusitis ◽

Specific Antibody ◽

Antibody Deficiency ◽

Specific Antibody Deficiency

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F1000Prime recommendation of Dub-seq: dual-barcoded shotgun expression library sequencing for high-throughput characterization of functional traits.

F1000 - Post-publication peer review of the biomedical literature ◽

10.3410/f.733787726.793556918 ◽

2019 ◽

Author(s):

Marvin Whiteley ◽

Kelly Michie ◽

Gina Lewin

Keyword(s):

Functional Traits ◽

High Throughput ◽

Expression Library

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Database of ab initio L-edge X-ray absorption near edge structure

Scientific Data ◽

10.1038/s41597-021-00936-5 ◽

2021 ◽

Vol 8 (1) ◽

Author(s):

Yiming Chen ◽

Chi Chen ◽

Chen Zheng ◽

Shyam Dwaraknath ◽

Matthew K. Horton ◽

...

Keyword(s):

High Throughput ◽

Scattering Theory ◽

Research Community ◽

Metal Compounds ◽

Edge Structure ◽

X Ray ◽

Initial Release ◽

Computational Workflow ◽

X Ray Absorption

AbstractThe L-edge X-ray Absorption Near Edge Structure (XANES) is widely used in the characterization of transition metal compounds. Here, we report the development of a database of computed L-edge XANES using the multiple scattering theory-based FEFF9 code. The initial release of the database contains more than 140,000 L-edge spectra for more than 22,000 structures generated using a high-throughput computational workflow. The data is disseminated through the Materials Project and addresses a critical need for L-edge XANES spectra among the research community.

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High-Throughput Synthesis and Characterization of a Combinatorial Materials Library in Bulk Alloys

Metallurgical and Materials Transactions A ◽

10.1007/s11661-021-06149-0 ◽

2021 ◽

Vol 52 (4) ◽

pp. 1159-1168

Author(s):

Lei Zhao ◽

Yuanxun Zhou ◽

Hui Wang ◽

Xuebin Chen ◽

Lixia Yang ◽

...

Keyword(s):

High Throughput ◽

Synthesis And Characterization ◽

Combinatorial Materials ◽

Bulk Alloys

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Tyrosinase Nanoparticles: Understanding the Melanogenesis Pathway by Isolating the Products of Tyrosinase Enzymatic Reaction

International Journal of Molecular Sciences ◽

10.3390/ijms22020734 ◽

2021 ◽

Vol 22 (2) ◽

pp. 734

Author(s):

Paul K. Varghese ◽

Mones Abu-Asab ◽

Emilios K. Dimitriadis ◽

Monika B. Dolinska ◽

George P. Morcos ◽

...

Keyword(s):

Catalytic Activity ◽

Large Scale ◽

Magnetic Beads ◽

Enzymatic Reaction ◽

Oculocutaneous Albinism ◽

Transmission Electron ◽

Hill Kinetics ◽

Rate Limiting ◽

Human Tyrosinase

Human Tyrosinase (Tyr) is the rate-limiting enzyme of the melanogenesis pathway. Tyr catalyzes the oxidation of the substrate L-DOPA into dopachrome and melanin. Currently, the characterization of dopachrome-related products is difficult due to the absence of a simple way to partition dopachrome from protein fraction. Here, we immobilize catalytically pure recombinant human Tyr domain (residues 19–469) containing 6xHis tag to Ni-loaded magnetic beads (MB). Transmission electron microscopy revealed Tyr-MB were within limits of 168.2 ± 24.4 nm while the dark-brown melanin images showed single and polymerized melanin with a diameter of 121.4 ± 18.1 nm. Using Hill kinetics, we show that Tyr-MB has a catalytic activity similar to that of intact Tyr. The diphenol oxidase reactions of L-DOPA show an increase of dopachrome formation with the number of MB and with temperature. At 50 °C, Tyr-MB shows some residual catalytic activity suggesting that the immobilized Tyr has increased protein stability. In contrast, under 37 °C, the dopachrome product, which is isolated from Tyr-MB particles, shows that dopachrome has an orange-brown color that is different from the color of the mixture of L-DOPA, Tyr, and dopachrome. In the future, Tyr-MB could be used for large-scale productions of dopachrome and melanin-related products and finding a treatment for oculocutaneous albinism-inherited diseases.

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