The Blood Lactate/Pyruvate Equilibrium Affair
Keyword(s):
The Lactate Shuttle hypothesis is supported by a variety of techniques including mass spectrometry analytics following infusion of carbon labeled isotopic tracers. However, there has been controversy over whether lactate tracers measure lactate (L) or pyruvate (P) turnover. Here we review the analytical errors, use of inappropriate tissue and animal models, failure to consider L and P pool sizes in modeling results, inappropriate tracer and blood sampling sites, and failure to anticipate roles of heart and lung parenchyma on L:P interactions. With support from magnetic resonance spectroscopy (MRS) and immunocytochemistry we conclude that carbon-labeled lactate tracers can be used to quantitate lactate fluxes.
2013 ◽
Vol 40
(12)
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pp. 1933-1937
1997 ◽
Vol 45
(10)
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pp. 4122-4126
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1981 ◽
Vol 46
(23)
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pp. 4770-4773
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