In vitro study of LDL transport under pressurized (convective) conditions

2007 ◽  
Vol 293 (1) ◽  
pp. H126-H132 ◽  
Author(s):  
Limary M. Cancel ◽  
Andrew Fitting ◽  
John M. Tarbell
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
In Vitro Study ◽  
Vitro Study ◽  
Bovine Aortic Endothelial Cell ◽  
Convective Conditions ◽  
Transport Pathways ◽  
Ldl Transport

It is difficult to assess the transport pathways that carry low-density lipoprotein (LDL) into the artery wall in vivo, and there has been no previous in vitro study that has examined transendothelial transport under physiologically relevant pressurized (convective) conditions. Therefore, we measured water, albumin, and LDL fluxes across bovine aortic endothelial cell (BAEC) monolayers in vitro and determined the relative contributions of vesicles, paracellular transport through “breaks” in the tight junction, and “leaky” junctions associated with dying or dividing cells. Our results show that leaky junctions are the dominant pathway for LDL transport (>90%) under convective conditions and that albumin also has a significant component of transport through leaky junctions (44%). Transcellular transport of LDL by receptor-mediated processes makes a minor contribution (<10%) to overall transport under convective conditions.

IN VITRO STUDY OF A NOVEL LOW-DENSITY LIPOPROTEIN ADSORBENT

2002 ◽  
Vol 30 (1) ◽  
pp. 53-61 ◽  
Author(s):  
Ning-Ning Cao ◽  
Yao-Ting Yu ◽  
Man-Yan Wang ◽  
Chang-Zhi Chen
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
In Vitro Study ◽  
Vitro Study ◽  
Low Density

Does Mercury Promote Lipid Peroxidation?: An In Vitro Study Concerning Mercury, Copper, and Iron in Peroxidation of Low-Density Lipoprotein

2004 ◽  
Vol 101 (2) ◽  
pp. 117-132 ◽  
Author(s):  
Kari Seppänen ◽  
Pasi Soininen ◽  
Jukka T. Salonen ◽  
Simo Lötjönen ◽  
Reino Laatikainen
Keyword(s):  
Lipid Peroxidation ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
In Vitro Study ◽  
Vitro Study ◽  
Low Density

scholarly journals In-Vitro Study of the Properties of Components for the Synthesis of Sorbent for Low-Density Lipoprotein Apheresis

Pharmacophore ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 37-41
Author(s):  
Anna Andreevna Bazhenova ◽  
Natalia Igorevna Guryanova ◽  
Gleb Sergeevich Guryanov ◽  
Heda Abdul Vahidovna Alieva ◽  
Diana Tamerlanovna Kachmazova ◽  
...  
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
In Vitro Study ◽  
Vitro Study ◽  
Low Density ◽  
Lipoprotein Apheresis

Antioxidant properties of aged garlic extract: an in vitro study incorporating human low density lipoprotein

Life Sciences ◽  
2003 ◽  
Vol 72 (14) ◽  
pp. 1583-1594 ◽  
Author(s):  
Stephanie A Dillon ◽  
Rajpal S Burmi ◽  
Gordon M Lowe ◽  
David Billington ◽  
Khalid Rahman
Keyword(s):  
Low Density Lipoprotein ◽  
Antioxidant Properties ◽  
Density Lipoprotein ◽  
In Vitro Study ◽  
Garlic Extract ◽  
Vitro Study ◽  
Low Density ◽  
Aged Garlic Extract ◽  
Aged Garlic

In vitro study of LDL transport under convective conditions

2006 ◽  
Vol 39 ◽  
pp. S376
Author(s):  
L.M. Cancel ◽  
A. Fitting ◽  
J.M. Tarbell
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Convective Conditions ◽  
Ldl Transport

Effects of TSH and calcitriol on bone metabolism: in vivo and in vitro study

2014 ◽  
Author(s):  
Ivo Dumic-Cule ◽  
Dunja Rogic ◽  
Damir Jezek ◽  
Lovorka Grgurevic ◽  
Slobodan Vukicevic
Keyword(s):  
Bone Metabolism ◽  
In Vitro Study ◽  
Vitro Study

scholarly journals Reductions of copper ion-mediated low-density lipoprotein (LDL) oxidations of trypsin inhibitors, the sweet potato root major proteins, and LDL binding capacities

2020 ◽  
Vol 61 (1) ◽  
Author(s):  
Yeh-Lin Lu ◽  
Chia-Jung Lee ◽  
Shyr-Yi Lin ◽  
Wen-Chi Hou
Keyword(s):  
Sweet Potato ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Trypsin Inhibitors ◽  
Water Soluble ◽  
Affinity Column ◽  
Low Density ◽  
Native Page

Abstract Background The root major proteins of sweet potato trypsin inhibitors (SPTIs) or named sporamin, estimated for 60 to 80% water-soluble proteins, exhibited many biological activities. The human low-density lipoprotein (LDL) showed to form in vivo complex with endogenous oxidized alpha-1-antitrypsin. Little is known concerning the interactions between SPTIs and LDL in vitro. Results The thiobarbituric-acid-reactive-substance (TBARS) assays were used to monitor 0.1 mM Cu2+-mediated low-density lipoprotein (LDL) oxidations during 24-h reactions with or without SPTIs additions. The protein stains in native PAGE gels were used to identify the bindings between native or reduced forms of SPTIs or soybean TIs and LDL, or oxidized LDL (oxLDL). It was found that the SPTIs additions showed to reduce LDL oxidations in the first 6-h and then gradually decreased the capacities of anti-LDL oxidations. The protein stains in native PAGE gels showed more intense LDL bands in the presence of SPTIs, and 0.5-h and 1-h reached the highest one. The SPTIs also bound to the oxLDL, and low pH condition (pH 2.0) might break the interactions revealed by HPLC. The LDL or oxLDL adsorbed onto self-prepared SPTIs-affinity column and some components were eluted by 0.2 M KCl (pH 2.0). The native or reduced SPTIs or soybean TIs showed different binding capacities toward LDL and oxLDL in vitro. Conclusion The SPTIs might be useful in developing functional foods as antioxidant and nutrient supplements, and the physiological roles of SPTIs-LDL and SPTIs-oxLDL complex in vivo will investigate further using animal models.

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