Recognition of consensus CHO structure in ligands for selectins by novel antibody against sialyl Lewis X

1995 ◽  
Vol 269 (4) ◽  
pp. H1282-H1287 ◽  
Author(s):  
T. Tamatani ◽  
M. Suematsu ◽  
K. Tezuka ◽  
N. Hanzawa ◽  
T. Tsuji ◽  
...  
Keyword(s):  
Sialyl Lewis X ◽  
Consensus Structure ◽  
Carbohydrate Structure ◽  
Lewis X ◽  
High Endothelial Venules ◽  
Cos Cells ◽  
Cell Lysate ◽  
Sialyl Lewis

The selectins (L, E, and P) play an important role in the earliest events of the inflammatory response, leading to the “rolling” phenomenon. All selectins react with sialyl Lewis X (SLex) in vitro, possibly suggesting that their ligands have a consensus structure. 2H5 is a monoclonal antibody against SLex that blocks L-selectin-mediated adhesion. 2H5 inhibited adhesion of HL-60 cells to P- and E-selectin-producing COS cells in vitro and immunoprecipitated a P-selectin glycoprotein ligand-1-like glycoprotein from HL-60 cell lysate, suggesting that it recognizes a functional consensus structure on the ligands for all selectins. 2H5 reacted not only with human but also with rat and mouse neutrophils. 2H5 is the first antibody against SLex that recognizes neutrophils of nonhuman mammals. The carbohydrate structure recognized by 2H5 was present not only on high endothelial venules of rat lymphoid organs but also on the endothelial cells of nonlymphoid organs. Furthermore, administration of the antibody markedly inhibited L- and P-selectin-mediated neutrophil rolling and adhesion in rat mesenteric venules in vivo. These results provide evidence for the presence of a consensus carbohydrate structure on the ligands for all selectins. The consensus structure thus has the potential to serve as a therapeutic target.

Sulfated Sialyl Lewis X, the Putative L-Selectin Ligand, Detected on Endothelial Cells of High Endothelial Venules by a Distinct Set of Anti-Sialyl Lewis X Antibodies

1997 ◽  
Vol 230 (3) ◽  
pp. 546-551 ◽  
Author(s):  
Chikako Mitsuoka ◽  
Naoko Kawakami-Kimura ◽  
Mikiko Kasugai-Sawada ◽  
Nozomu Hiraiwa ◽  
Ken'ichi Toda ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Sialyl Lewis X ◽  
Lewis X ◽  
High Endothelial Venules ◽  
Sialyl Lewis ◽  
Selectin Ligand

In Vitro Selection of the RNA Aptamer against the Sialyl Lewis X and Its Inhibition of the Cell Adhesion

2001 ◽  
Vol 281 (1) ◽  
pp. 237-243 ◽  
Author(s):  
Sunjoo Jeong ◽  
Tae-Yeon Eom ◽  
Se-Jin Kim ◽  
Seong-Wook Lee ◽  
Jaehoon Yu
Keyword(s):  
Cell Adhesion ◽  
In Vitro Selection ◽  
Sialyl Lewis X ◽  
Rna Aptamer ◽  
Lewis X ◽  
Sialyl Lewis ◽  
Selection Of

scholarly journals Mechanical Shedding of L-selectin from the Neutrophil Surface during Rolling on Sialyl Lewis x under Flow

2006 ◽  
Vol 282 (7) ◽  
pp. 4812-4820 ◽  
Author(s):  
Dooyoung Lee ◽  
Joanne B. Schultz ◽  
Philip A. Knauf ◽  
Michael R. King
Keyword(s):  
Flow Chamber ◽  
Mechanical Force ◽  
Mitogen Activated Protein Kinase ◽  
Sialyl Lewis X ◽  
Lewis X ◽  
Rolling Velocity ◽  
Sialyl Lewis ◽  
Mitogen Activated Protein ◽  
Neutrophil Surface

The interaction of L-selectin expressed on leukocytes with endothelial cells leads to capture and rolling and is critical for the recruitment of leukocytes into sites of inflammation. It is known that leukocyte activation by chemoattractants, the change of osmotic pressure in cell media, or cross-linking of L-selectin all result in rapid shedding of L-selectin. Here we present a novel mechanism for surface cleavage of L-selectin on neutrophils during rolling on a sialyl Lewis x-coated surface that involves mechanical force. Flow cytometry and rolling of neutrophils labeled with Qdot®-L-selectin antibodies in an in vitro flow chamber showed that the mechanical shedding of L-selectin occurs during rolling and depends on the amount of shear applied. In addition, the mechanical L-selectin shedding causes an increase in cell rolling velocity with rolling duration, suggesting a gradual loss of L-selectin and is mediated by p38 mitogen-activated protein kinase activation. Thus, these data show that mechanical force induces the cleavage of L-selectin from the neutrophil surface during rolling and therefore decreases the adhesion of cells to a ligand-presenting surface in flow.

scholarly journals Identification of a Major Carbohydrate Capping Group of the L-selectin Ligand on High Endothelial Venules in Human Lymph Nodes as 6-Sulfo Sialyl Lewis X

1998 ◽  
Vol 273 (18) ◽  
pp. 11225-11233 ◽  
Author(s):  
Chikako Mitsuoka ◽  
Mikiko Sawada-Kasugai ◽  
Keiko Ando-Furui ◽  
Mineko Izawa ◽  
Hayao Nakanishi ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Sialyl Lewis X ◽  
Lewis X ◽  
High Endothelial Venules ◽  
Sialyl Lewis ◽  
Selectin Ligand

Accumulation of liposome with Sialyl Lewis X to inflammation and tumor region: Application to in vivo bio-imaging

2007 ◽  
Vol 353 (3) ◽  
pp. 553-558 ◽  
Author(s):  
Masahiko Hirai ◽  
Hideki Minematsu ◽  
Naoko Kondo ◽  
Kazunori Oie ◽  
Koichi Igarashi ◽  
...  
Keyword(s):  
Sialyl Lewis X ◽  
Lewis X ◽  
Sialyl Lewis ◽  
Bio Imaging ◽  
Tumor Region

scholarly journals Evaluation of Strategies Aimed at Improving Liver Progenitor Cell Rolling and Subsequent Adhesion to the Endothelium

2020 ◽  
Vol 29 ◽  
pp. 096368972091270
Author(s):  
Pierre Edouard Dollet ◽  
Mei Ju Hsu ◽  
Jérôme Ambroise ◽  
Milena Rozzi ◽  
Joachim Ravau ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Cell Adhesion ◽  
Alternative Methods ◽  
Sialyl Lewis X ◽  
Thermosensitive Polymer ◽  
Lewis X ◽  
Promising Alternative ◽  
Sialyl Lewis ◽  
Selectin Ligand

Adult-derived human liver stem/progenitor cells (ADHLSCs) are a promising alternative to orthotopic liver transplantation in the treatment of inborn errors of metabolism. However, as is the case with many mesenchymal stromal cells, ADHLSCs have shown a low level of engraftment, which could be explained by the fact that they lack expression of selectin ligand and LFA-1 and only slightly express VLA- 4, molecules that have been shown to be involved in cell adhesion to the endothelium. In this paper, we have investigated strategies to increase their rolling and adhesion during the homing process by (1) adding a selectin ligand (Sialyl Lewis X) to their surface using biotinyl- N-hydroxy-succinimide–streptavidin bridges, and (2) protecting the adhesion proteins from trypsinization-induced damage using a thermosensitive polymer for cell culture and a nonenzymatic cell dissociation solution (CDS) for harvest. Despite increasing adhesion of ADHLSCs to E-selectin during an adhesion test in vitro performed under shear stress, the addition of Sialyl Lewis X did not increase adhesion to endothelial cells under the same conditions. Cultivating cells on a thermosensitive polymer and harvesting them with CDS increased their adhesion to endothelial cells under noninflammatory conditions, compared to the use of trypsin. However, we were not able to demonstrate any improvement in cell adhesion to the endothelium following culture on polymer and harvest with CDS, suggesting that alternative methods of improving engraftment still need to be evaluated.

scholarly journals 1001-33 In vivo microtubule binding to inflammatory endothelium via selectin targeting by Sialyl Lewis X

2004 ◽  
Vol 43 (5) ◽  
pp. A8 ◽  
Author(s):  
Erxiong Lu ◽  
Eric M Tom ◽  
Matundu M Felix ◽  
Joan Gretton ◽  
Rekhi P Varghese ◽  
...  
Keyword(s):  
Sialyl Lewis X ◽  
Lewis X ◽  
Microtubule Binding ◽  
Sialyl Lewis

L-selectin can mediate leukocyte rolling in untreated mesenteric venules in vivo independent of E- or P-selectin

Blood ◽  
1993 ◽  
Vol 82 (5) ◽  
pp. 1632-1638 ◽  
Author(s):  
K Ley ◽  
TF Tedder ◽  
GS Kansas
Keyword(s):  
Side Branch ◽  
Sialyl Lewis X ◽  
Leukocyte Rolling ◽  
Endothelial Lining ◽  
Lectin Domain ◽  
Sialyl Lewis ◽  
Human Granulocytes ◽  
Lymphocytic Cell Line

Abstract Granulocyte recruitment during the acute inflammatory response is initiated by their rolling along the endothelial lining of postcapillary venules. To determine whether expression of L-selectin alone is sufficient for rolling, the murine pre-B lymphocytic cell line 300.19, which does not bind E- or P-selectin, was transfected with human L-selectin cDNA, which led to stable L-selectin expression at a level similar to that of blood lymphocytes. Fluorescent-labeled cells were infused retrogradely into a side branch of the superior mesenteric artery of anesthetized rats. In venules of the mesenteric membrane, leukocyte rolling occurs without intentional stimulation. On average, 17% +/- 6% of L-selectin transfectants rolled in the observed venules, while mock-transfected cells did not roll. Rolling of L-selectin transfectants began approximately 20 minutes after surgery and continued for at least 120 minutes. In contrast, HL-60 promyelocytes, which express sialyl-Lewis(x) tetrasaccharide (sLe(x)) but not L- selectin and that bind to E- and P-selectin in vitro, did not roll between 20 and 120 minutes, but some HL-60 cells rolled at very early (< 20 minutes) and late (> 2 hours) time points. Pretreatment with either of two function-blocking monoclonal antibodies recognizing the lectin domain of L-selectin completely blocked rolling of L-selectin transfectants and sharply reduced (by 70%) rolling of isolated human granulocytes. Taken together, these results show that L-selectin can mediate leukocyte rolling by virtue of its lectin activity.

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