ET-1 in the myocardial interstitium: relation to myocyte ECE activity and expression

2000 ◽  
Vol 278 (6) ◽  
pp. H2050-H2056 ◽  
Author(s):  
Adviye Ergul ◽  
C. Allyson Walker ◽  
Aron Goldberg ◽  
Simona C. Baicu ◽  
Jennifer W. Hendrick ◽  
...  
Keyword(s):  
Heart Failure ◽  
Congestive Heart Failure ◽  
Plasma Levels ◽  
Left Ventricular ◽  
Converting Enzyme ◽  
Endothelin 1 ◽  
Endothelin Converting Enzyme

Increased plasma levels of endothelin-1 (ET-1) have been identified in congestive heart failure (CHF), but local myocardial interstitial ET-1 levels and the relation to determinants of ET-1 synthesis remain to be defined. Accordingly, myocardial interstitial ET-1 levels and myocyte endothelin-converting enzyme (ECE)-1 activity and expression with the development of CHF were examined. Pigs were instrumented with a microdialysis system to measure myocardial interstitial ET-1 levels with pacing CHF (240 beats/min, 3 wk; n = 9) and in controls ( n = 14). Plasma ET-1 was increased with CHF (15 ± 1 vs. 9 ± 1 fmol/ml, P < 0.05) as was total myocardial ET-1 content (90 ± 15 vs. 35 ± 5 fmol/g, P < 0.05). Paradoxically, myocardial interstitial ET-1 was decreased in CHF (32 ± 4 vs. 21 ± 2 fmol/ml, P < 0.05), which indicated increased ET-1 uptake by the left ventricular (LV) myocardium with CHF. In isolated LV myocyte preparations, ECE-1 activity was increased by twofold with CHF ( P < 0.05). In LV myocytes, both ECE-1a and ECE-1c mRNAs were detected, and ECE-1a expression was upregulated fivefold in CHF myocytes ( P < 0.05). In conclusion, this study demonstrated compartmentalization of ET-1 in the myocardial interstitium and enhanced ET-1 uptake with CHF. Thus a local ET-1 system exists at the level of the myocyte, and determinants of ET-1 biosynthesis are selectively regulated within this myocardial compartment in CHF.

Enhancement of Endothelin Converting Enzyme and Endothelin Receptor Subtypes in Human Myocardium with Congestive Heart Failure

2000 ◽  
Vol 6 (S2) ◽  
pp. 608-609
Author(s):  
J. Lin ◽  
C Wei
Keyword(s):  
Heart Failure ◽  
Congestive Heart Failure ◽  
Cardiac Tissue ◽  
Vascular Endothelial Cells ◽  
Normal Subjects ◽  
Human Myocardium ◽  
Endothelin Receptors ◽  
Receptor Subtypes ◽  
Converting Enzyme ◽  
Endothelin Converting Enzyme

Endothelin-1 (ET-1) is a potent endothelial cell-drived vasoconstrictive peptide which is increased in congestive heart failure (CHF). ET-1 is converted from its precursor big ET-1 by activation of endothelin converting enzyme (ECE). ET-1 binding to ET-A receptor in vascular smooth muscle cells stimulates vasoconstriction and binding to ET-B receptor in vascular endothelial cells mediates vasodilation. In previous studies, we and others demonstrated that plasma ET-1 was significantly increased in congestive heart failure. However, the presentation and localization of endothelin converting enzyme and endothelin receptors (ET-A and ET-B) in human cardiac tissue with and without heart failure remain unclear. Therefore, the current study was designed to investigate the expression and localization of endothelin receptors and endothelin converting enzyme in human myocardium in the absence or presence of congestive heart failure.Human atrial tissues (n=6) were obtained from normal subjects and end-stage CHF patients during cardiac transplantation. The expression of ECE, ET-A and ET-B were determined by immunohistochemical staining (IHCS).

Plasma levels of endothelin-1 at rest and after exercise in patients with moderate congestive heart failure

1995 ◽  
Vol 51 (3) ◽  
pp. 267-272 ◽  
Author(s):  
Pascal de Groote ◽  
Alain Millaire ◽  
André Racadot ◽  
Eric Decoulx ◽  
Gérard Ducloux
Keyword(s):  
Heart Failure ◽  
Congestive Heart Failure ◽  
Plasma Levels ◽  
Endothelin 1

Isometric Handgrip Exercise Increases Endothelin-1 Plasma Levels in Patients With Chronic Congestive Heart Failure

1997 ◽  
Vol 79 (9) ◽  
pp. 1261-1263 ◽  
Author(s):  
Enrico Mangieri ◽  
Gaetano Tanzilli ◽  
Francesco Barillà ◽  
Massimo Ciavolella ◽  
Gianluca Serafini ◽  
...  
Keyword(s):  
Heart Failure ◽  
Congestive Heart Failure ◽  
Plasma Levels ◽  
Chronic Congestive Heart Failure ◽  
Handgrip Exercise ◽  
Isometric Handgrip ◽  
Endothelin 1 ◽  
Isometric Handgrip Exercise

Plasma levels and molecular forms of proatrial natriuretic peptides in healthy subjects and in patients with congestive heart failure

1996 ◽  
Vol 148 (1) ◽  
pp. 51-57 ◽  
Author(s):  
C Azizi ◽  
G Maistre ◽  
H Kalotka ◽  
R Isnard ◽  
C Barthélemy ◽  
...  
Keyword(s):  
Heart Failure ◽  
Congestive Heart Failure ◽  
Plasma Levels ◽  
Healthy Subjects ◽  
Plasma Concentrations ◽  
Gel Filtration ◽  
Heart Association ◽  
Left Ventricular ◽  
Molecular Forms ◽  
Asymptomatic Patients

Abstract A specific and sensitive radioimmunoassay (RIA) for the N-terminal fragment of proatrial natriuretic peptide (NproANP) was developed. Antiserum raised in rabbits against a mixture enriched with prohormone was 100% cross-reactive with human proANP(1–30). Plasma concentrations of proANP(1–30) and ANP immunoreactivities (ir-) were simultaneously measured in healthy subjects and patients with congestive heart failure (CHF; 26 dilated cardiomyopathy and 5 ischemic heart disease). High plasma levels of both ir-proANP(1–30) and ir-ANP were detected in CHF patients. Circulating ir-ANP levels were elevated in New York Heart Association functional Classes II and III patients but not in Class I patients. However, plasma levels of ir-proANP(1–30) were higher in asymptomatic patients than in healthy subjects, and markedly increased in patients of Classes II and III. Analysis of ir-proANP(1–30) by gel filtration chromatography or reverse-phase high pressure liquid chromatography revealed a 10 kDa peptide circulating as a distinct entity. These findings indicate that: (i) the most probable form of NproANP in human plasma is a 10 kDa peptide and (ii) in CHF patients the rise in plasma ir-proANP(1–30) levels is more pronounced than the variation in plasma ir-ANP. Thus, NproANP plasma levels may prove to be a more sensitive marker of left ventricular dysfunction than ANP. Journal of Endocrinology (1996) 148, 51–57

scholarly journals Inhibition of both neutral endopeptidase and endothelin converting enzyme lowers pulmonary pressures in congestive heart failure

2003 ◽  
Vol 41 (6) ◽  
pp. 266 ◽  
Author(s):  
Kenneth Dickstein ◽  
Hanka de Voogd ◽  
Milutin Miric ◽  
Roland Willenbrock ◽  
Veselin Mitrovic ◽  
...  
Keyword(s):  
Heart Failure ◽  
Congestive Heart Failure ◽  
Neutral Endopeptidase ◽  
Converting Enzyme ◽  
Endothelin Converting Enzyme

scholarly journals Effects of a Specific Endothelin-Converting Enzyme Inhibitor on Cardiac, Renal, and Neurohumoral Functions in Congestive Heart Failure

Circulation ◽  
1999 ◽  
Vol 99 (4) ◽  
pp. 570-577 ◽  
Author(s):  
Atsuyuki Wada ◽  
Takayoshi Tsutamoto ◽  
Masato Ohnishi ◽  
Masahide Sawaki ◽  
Daisuke Fukai ◽  
...  
Keyword(s):  
Heart Failure ◽  
Congestive Heart Failure ◽  
Enzyme Inhibitor ◽  
Converting Enzyme ◽  
Converting Enzyme Inhibitor ◽  
Endothelin Converting Enzyme
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