Formation and Binding of Histamine by Free Mast Cells of Rat Peritoneal Fluid

1956 ◽  
Vol 186 (2) ◽  
pp. 199-202 ◽  
Author(s):  
Richard W. Schayer
Keyword(s):  
Mast Cells ◽  
Peritoneal Fluid ◽  
Histidine Decarboxylase ◽  
Cell Suspensions ◽  
Stable Form ◽  
Effects Of Temperature ◽  
Temperature Time ◽  
Considerable Loss ◽  
Number Of Cells

Suspensions of the cells of rat peritoneal fluid have a marked ability to decarboxylate C14 l-histidine and bind the resulting histamine in stable form. The evidence is strong that this activity is due almost entirely to mast cells; these constitute about 4% of the total number of cells present. The effects of temperature, time, and ph on this process were found to be characteristic of enzymic reactions. Preincubation of cell suspensions with nonisotopic histamine had no effect on the formation and binding of C14 histamine; this is evidence that exogenous histamine cannot enter the bound condition in free mast cells. The effects of histidine analogs on binding is described. Disruption of the mast cells results in a considerable loss in histamine forming activity. However, a fairly stable, soluble histidine decarboxylase can be obtained from the disrupted cells. A new method for determination of histamine as the S35 dibenzenesulfonyl derivative is described.

scholarly journals Quantitation of mast cell heparin by flow cytofluorometry.

1976 ◽  
Vol 24 (12) ◽  
pp. 1231-1238 ◽  
Author(s):  
L Enerbäck ◽  
G Berlin ◽  
I Svensson ◽  
I Rundquist
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Mixed Cell ◽  
Flow Cytofluorometry ◽  
Frequency Distributions ◽  
Cell Counts ◽  
Mixed Cell Population ◽  
Number Of Cells ◽  
Excellent Reproducibility

Mast cells can be automatically identified in a mixed cell population by flow cytofluorometry after Berberine sulphate staining. Volume specific counts of the total number of cells and number of mast cells, as well as frequency distributions of fluorescence intensities of mast cells, based on a large number of cells, can be rapidly obtained. Results obtained by microscope fluorometry of cells identified by phase contrast microscopy showviously published results it may be inferred that the fluorescence intensity of individual mast cells is proportional to mast cell heparin content. The automated cell counts correlated very well with manual hemocytometer counts. Both cell counts and the determination of mean mast cell fluorescence showed excellent reproducibility.

scholarly journals DETERMINATION OF THE COEFFICIENT OF HEAT TRANSFER DURING COOLING OF SILVER THERMO PROBES, TAKING INTO ACCOUNT THE EFFECTS OF TEMPERATURE-TIME DELAY

2017 ◽  
Vol 39 (5) ◽  
pp. 41-47 ◽  
Author(s):  
E. N. Zotov ◽  
A. A. Moskalenko A.A. ◽  
O. V. Rasumtseva ◽  
L. M. Protsenko
Keyword(s):  
Heat Transfer ◽  
Heat Flux Density ◽  
Cooling Capacity ◽  
Heat Transfer Process ◽  
Special Program ◽  
Nonstationary Heat Transfer ◽  
Density Surface ◽  
Effects Of Temperature ◽  
Temperature Time

Existing methods for determining the characteristics of the nonstationary heat transfer process (temperature field, heat transfer coefficient, heat flux density, surface temperature) are considered and analyzed when cooling silver spherical and cylindrical thermo-probes. New analytical solutions are proposed using a special program IQLab, which increase the accuracy of calculations when testing the cooling capacity of various liquids. The results of the calculations are compared with the experimental data.  

scholarly journals Measurement of Homocysteine and Other Aminothiols in Plasma: Advantages of Using Tris(2-carboxyethyl)phosphine as Reductant Compared with Tri-n-butylphosphine

2001 ◽  
Vol 47 (10) ◽  
pp. 1821-1828 ◽  
Author(s):  
Jakub Krijt ◽  
Martina Vacková ◽  
Viktor Kožich
Keyword(s):  
Low Molecular Weight ◽  
Total Glutathione ◽  
Deming Regression ◽  
Mixed Disulfides ◽  
Total Homocysteine ◽  
The Mean ◽  
Effects Of Temperature ◽  
Temperature Time ◽  
Reliable Methods

Abstract Background: Aminothiols have been implicated in the pathogenesis of arteriosclerosis, and reliable methods are needed to determine their concentrations in body fluids. We present a comparison of two analytical methods and focus on the reduction of low-molecular weight and protein-mixed disulfides of homocysteine, cysteine, cysteinyl-glycine, and glutathione. Methods: The plasma total aminothiol profile was determined by HPLC with fluorescence detection after derivatization with ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonate. Disulfides and protein-bound aminothiols were reduced by either tri-n-butylphosphine (the TBP method) or tris(2-carboxyethyl)phosphine (the TCEP method); the effects of temperature, time of reduction, and concentration of reductants were evaluated. Results: The intraassay imprecision (CV) was <3% for all aminothiols using both methods. The interassay CVs for total cysteine (tCys), total cysteinyl-glycine (tCys-Gly), and total homocysteine (tHcy) were <4% and <8% for the TCEP and TBP methods, respectively, whereas for total glutathione (tGSH) the interassay CV was >12% for both methods. Deming regression and Bland–Altman difference plots showed positive biases for total aminothiol concentrations determined by the TCEP method relative to the TBP method. The mean proportional biases were 65%, 27%, 6%, and 60% for tCys, tCys-Gly, tHcy, and tGSH, respectively. The calculated concentrations of total aminothiols by the TCEP method were less influenced by changes in temperature and concentration of reducing agent or by calibrator matrix. Conclusions: The agreement between the TCEP and TBP methods was considerably lower for the determination of tCys, tCys-Gly, and tGSH than for tHcy. For total-aminothiol determination, the TCEP method yields better reproducibility and is more robust than the TBP method.

PENENTUAN KRITERIA PENILAIAN KESAN JUMLAH LEUKOSIT PADA PEMERIKSAAN APUSAN DARAH TEPI

2018 ◽  
Vol 3 (2) ◽  
pp. 52-61
Author(s):  
Dzikra Arwie ◽  
Islawati
Keyword(s):  
Data Analysis ◽  
Statistical Analysis ◽  
Blood Cells ◽  
Blood Smear ◽  
White Blood Cells ◽  
Peripheral Blood Smear ◽  
Field Of View ◽  
Laboratory Observation ◽  
Number Of Cells

Leukocytes or white blood cells have a characteristic characteristic of different cells. Determination of the impression of the number of leukocytes is determined in the number of cells in the field of view. While the number of viewable field cells expressed is still quite varied. The purpose of this study was to determine the number of leukocytes in the field of view and expressed the impression of a sufficient amount. This research was conducted at the Laboratory of Health Analyst Department Panrita Husada Bulukumba on 9 April 2017 to 14 July 2017. This type of research is a laboratory observation that aims to determine the criteria for assessing the impression of the number of leukocytes on a peripheral blood smear. Data analysis using statistical analysis is the average and standard deviations to determine the impression of the number of leukocytes and use 3 inspection zones. The results of this study obtained results in zone IV the number of leukocyte impressions said to be sufficient was 7-10, in zone V the number of leukocyte impressions said to be sufficient was 4-9, and in zone VI the number of leukocyte impressions said to be sufficient was 3-8.  

EFFECTS OF TEMPERATURE-TIME COMBINATIONS ON DONENESS AND YIELDS OF WATER-COOKED BROILER THIGHS

1975 ◽  
Vol 40 (1) ◽  
pp. 129-132 ◽  
Author(s):  
C. E. LYON ◽  
B. G. LYON ◽  
A. A. KLOSE ◽  
J. P. HUDSPETH
Keyword(s):  
Effects Of Temperature ◽  
Temperature Time

Mast cells and histamine concentration in muscle and liver of dystrophic mice

1964 ◽  
Vol 206 (2) ◽  
pp. 338-340 ◽  
Author(s):  
Pierre Bois
Keyword(s):  
Mast Cells ◽  
Histidine Decarboxylase ◽  
Binding Capacity ◽  
The Other ◽  
Decarboxylase Activity ◽  
Histamine Concentration ◽  
Other Hand ◽  
Histamine Binding ◽  
Dystrophic Mice ◽  
Histidine Decarboxylase Activity

The distribution of mast cells in muscle and liver of dystrophic mice was studied; histamine and histidine decarboxylase activity was also measured in the same tissues. Mast cells were significantly more numerous in dystrophic muscles. On the other hand, very few cells could be counted in the liver of either control or dystrophic animals. Histamine concentration was higher in muscle and liver of dystrophic mice; no visible increase in histidine decarboxylase activity could be measured by the methods used. It is concluded that histamine-binding capacity is increased in some tissues of dystrophic mice.

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