Specificity of sugar transport by the intestine of the hamster

1960 ◽  
Vol 198 (1) ◽  
pp. 99-102 ◽  
Author(s):  
T. Hastings Wilson ◽  
Bernard R. Landau
Keyword(s):  
Small Intestine ◽  
Transport System ◽  
Concentration Gradient ◽  
Sugar Transport ◽  
Intestinal Wall ◽  
Sugar Derivatives

The specificity of the sugar transport system of the hamster small intestine was tested with 20 sugars and sugar derivatives not previously tested in this system. The absorption of sugars across the intestinal wall against a concentration gradient was tested with the everted sac technique in vitro. 3-Deoxyglucose, 4-0-methylgalactose, 6-deoxy-6-fluoroglucose and α-methylglucoside were transported while a variety of other sugars were not. From the data derived from the study of a total of 49 sugars tested in this system, certain generalizations are made as to the structural limitations of the sugar-absorbing capacity of the hamster intestine.

Transport of l-tyrosine by the small intestine in vitro

1960 ◽  
Vol 199 (1) ◽  
pp. 127-130 ◽  
Author(s):  
Edmund C. C. Lin ◽  
T. Hastings Wilson
Keyword(s):  
Small Intestine ◽  
Amino Acid ◽  
Transport System ◽  
Concentration Gradient ◽  
Animal Species

l-Tyrosine was absorbed against a concentration gradient by sacs of isolated small intestine obtained from several animal species. During absorption the tissue segments accumulated the amino acid to concentrations as high as nine times that in the suspending medium. In the case of the hamster the rate of transport was maximal in the mid portion of the small intestine. Studies on specificity of the transport system showed that l-tyrosine and dl-meta-tyrosine were transported while no activity was observed with d-tyrosine, dl-ortho-tyrosine, and 3,5-diiodo-l-tyrosine. Transport of l-tyrosine was inhibited by l-phenylalanine and l-methionine but not by d-phenylalanine. High tyrosine diet did not alter the rate of the in vitro transport of this amino acid in the rat.

In vitro absorption of bile salts by small intestine of rats and guinea pigs

1961 ◽  
Vol 200 (2) ◽  
pp. 313-317 ◽  
Author(s):  
Leon Lack ◽  
I. M. Weiner
Keyword(s):  
Small Intestine ◽  
Bile Acid ◽  
Active Transport ◽  
Sodium Azide ◽  
Concentration Gradient ◽  
Guinea Pigs ◽  
Bile Salts ◽  
Ileal Segment

The transport of taurocholic and glycocholic acids by the small intestine of rats and guinea pigs against a concentration gradient was studied by the everted gutsac technique. Transport of these substances is limited to the distal ileal segment. This transport is inhibited by anoxia, dinitrophenol and sodium azide. The system has a transport maximum. On the basis of these criteria bile acid reabsorption is considered to occur by active transport.

Factors controlling B12 uptake by intestinal sacs in vitro

1960 ◽  
Vol 198 (1) ◽  
pp. 103-107 ◽  
Author(s):  
Elliott W. Strauss ◽  
T. Hastings Wilson
Keyword(s):  
Small Intestine ◽  
Low Temperature ◽  
Guinea Pig ◽  
Calcium Ion ◽  
Intrinsic Factor ◽  
Intestinal Wall ◽  
Vitamin B ◽  
Anaerobic Conditions ◽  
Intestinal Uptake

Sacs of everted small intestine were incubated in bicarbonate-saline containing radioactive vitamin B12 with or without a source of gastric intrinsic factor (IF). In both the hamster and guinea pig the lowest ileum was most active in B12 uptake in the presence of intrinsic factor, the upper jejunum showing little or no uptake. Low temperature and anaerobic conditions completely abolished the stimulatory effect of IF on B12 uptake. Intrinsic factor did not bind to the intestinal wall in the absence of B12 (even in the presence of calcium ion) as the IF activity could be completely removed by gentle washing of the tissue. The vitamin and intrinsic factor must be present together to cause intestinal uptake of B12.

Effect of VIP on Sugar Transport in Rabbit Small Intestine in vitro

1990 ◽  
Vol 37 (1-10) ◽  
pp. 123-129 ◽  
Author(s):  
M. P. Arruebo ◽  
V. Sorribas ◽  
M. J. Rodriguez-Yoldi ◽  
M. D. Murillo ◽  
Ana Isabel Alcalde
Keyword(s):  
Small Intestine ◽  
Sugar Transport ◽  
Rabbit Small Intestine

scholarly journals Metabolism of ε-(γ-L-glutamyl)-L-lysine in the rat

1975 ◽  
Vol 34 (2) ◽  
pp. 291-296 ◽  
Author(s):  
G. Raczyński ◽  
M. Snochowski ◽  
S. Buraczewski
Keyword(s):  
Small Intestine ◽  
Intestinal Wall ◽  
Rat Small Intestine ◽  
Blood Proteins ◽  
Vitro Experiment ◽  
Comparative Tests ◽  
In Vitro Experiment ◽  
Body Tissues ◽  
Everted Sacs

1. A study was made of the metabolism of ɛ-(γ-L-glutamyl)-L[4, 5-3H]lysine (GL) in the rat.2. The compound was largely absorbed from the intestine and metabolized. Labelled lysine was incorporated into blood proteins.3. In an in vitro experiment with everted sacs of rat small intestine, GL passed through the intestinal wall unchanged.4. The results of comparative tests using homogenates of different body tissues indicated that the kidneys were particularly active in hydrolysing GL. Their activity was nine times greater than that of the liver and eighteen times greater than that of the small intestine.

A common pathway for sugar transport in hamster intestine

1961 ◽  
Vol 200 (1) ◽  
pp. 111-116 ◽  
Author(s):  
Charles R. Jorgensen ◽  
Bernard R. Landau ◽  
T. Hastings Wilson
Keyword(s):  
Small Intestine ◽  
Transport Mechanism ◽  
Sugar Transport ◽  
The Other ◽  
Common Pathway ◽  
In Vitro Method ◽  
Single Segment ◽  
Other Hand

The competition between different sugars for the transport mechanism of hamster small intestine was tested with an in vitro method which allowed the use of a single segment of intestine for both control and experimental periods. The transport of the test sugar d-galactose was inhibited by other sugars known to be actively absorbed by the intestine; namely, d-glucose, α-methyl-d-glucoside, i-deoxy-d-glucose, 6-deoxy-d-glucose and 3-o-methyl-d-glucose. On the other hand d-mannose and d-xylose, two sugars not actively transported, did not inhibit d-galactose absorption. In addition, sugars known to be actively absorbed produced an inhibition of transport of d-glucose and 6-deoxy-d-glucose when these were selected as test sugars. The results of these experiments are consistent with the view that all transported sugars compete for a common pathway in hamster intestine. Various hypotheses of sugar transport are discussed in light of the present data.

Effects of metabolic disturbances on potential difference across intestinal wall of rat

1963 ◽  
Vol 204 (1) ◽  
pp. 105-108 ◽  
Author(s):  
Masashi Sawada ◽  
Tomoaki Asano
Keyword(s):  
Small Intestine ◽  
Low Temperature ◽  
Intestinal Wall ◽  
Potential Difference ◽  
Rapid Decline ◽  
Serosal Side ◽  
Metabolic Disturbances ◽  
Temperature Dependent ◽  
Mucosal Side

The potential difference across the wall of the small intestine was determined in vitro under a variety of conditions using rats. When the normal Ringer's containing 200 mg/100 ml glucose was applied on both sides of the wall, the potential difference attained 5–9 mv, the serosal side being positive. The potential difference was temperature dependent, becoming reduced at low temperature, the temperature coefficient being 1.7 between 40 and 34 C. The potential difference was inhibited with 0.1 mm monoiodoacetic acid, 1 mm sodium azide, 0.1 mm dinitrophenol, and 50 µm ouabain applied on the mucosal side. Withdrawal and restitution of 200 mg/100 ml glucose on the mucosal side induced a rapid decline and recovery of the potential difference. The lowered potential difference was partially recovered by 200 mg/100 ml galactose but not by sorbitol.

scholarly journals Part of quercetin absorbed in the small intestine is conjugated and further secreted in the intestinal lumen

1999 ◽  
Vol 277 (1) ◽  
pp. G120-G126 ◽  
Author(s):  
Vanessa Crespy ◽  
Christine Morand ◽  
Claudine Manach ◽  
Catherine Besson ◽  
Christian Demigne ◽  
...  
Keyword(s):  
Small Intestine ◽  
Intestinal Wall ◽  
Intestinal Lumen ◽  
Intestinal Cells ◽  
Biliary Duct ◽  
In Situ Perfusion ◽  
Perfusion Period ◽  
Hydrolysis Of

Rutin and quercetin absorption and metabolism were investigated in rats after in situ perfusion of jejunum plus ileum (15 nmol/min). In contrast to rutin, a high proportion of quercetin (two-thirds) disappeared during perfusion, reflecting extensive transfer into the intestinal wall. Net quercetin absorption was not complete (2.1 nmol/min), inasmuch as 52% were reexcreted in the lumen as conjugated derivatives (7.7 nmol/min). Enterohepatic recycling contribution of flavonoids was excluded by catheterization of the biliary duct before perfusion. After a 30-min perfusion period, 0.71 μM of quercetin equivalents were detected in plasma, reflecting a significant absorption from the small intestine. The differential hydrolysis of effluent samples by glucuronidase and/or sulfatase indicates that the conjugated forms released in the lumen were 1) glucuronidated derivatives of quercetin and of its methoxylated forms (64%) and 2) sulfated form of quercetin (36%). In vitro quercetin glucuronides synthetized using jejunal and ileal microsomal fractions were similar to those recovered in the effluent of perfusion. These data suggest that glucuronidation and sulfatation take place in intestinal cells, whereas no glucurono-sulfoconjugates could be detected in the effluent. The present work shows that a rapid quercetin absorption in the small intestine is very effective together with its active conjugation in intestinal cells.

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