scholarly journals Transcriptome-wide RNA sequencing analysis of rat skeletal muscle feed arteries. I. Impact of obesity

2014 ◽  
Vol 116 (8) ◽  
pp. 1017-1032 ◽  
Author(s):  
Nathan T. Jenkins ◽  
Jaume Padilla ◽  
Pamela K. Thorne ◽  
Jeffrey S. Martin ◽  
R. Scott Rector ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Endothelial Cell ◽  
Rna Sequencing ◽  
Differentially Expressed ◽  
Aortic Endothelial Cell ◽  
Oletf Rats ◽  
Long Evans ◽  
Feed Arteries ◽  
Skeletal Muscle Feed Arteries ◽  
Aortic Endothelial

We employed next-generation RNA sequencing (RNA-Seq) technology to determine the influence of obesity on global gene expression in skeletal muscle feed arteries. Transcriptional profiles of the gastrocnemius and soleus muscle feed arteries (GFA and SFA, respectively) and aortic endothelial cell-enriched samples from obese Otsuka Long-Evans Tokushima Fatty (OLETF) and lean Long-Evans Tokushima Otsuka (LETO) rats were examined. Obesity produced 282 upregulated and 133 downregulated genes in SFA and 163 upregulated and 77 downregulated genes in GFA [false discovery rate (FDR) < 10%] with an overlap of 93 genes between the arteries. In LETO rats, there were 89 upregulated and 114 downregulated genes in the GFA compared with the SFA. There were 244 upregulated and 275 downregulated genes in OLETF rats (FDR < 10%) in the GFA compared with the SFA, with an overlap of 76 differentially expressed genes common to both LETO and OLETF rats in both the GFA and SFA. A total of 396 transcripts were found to be differentially expressed between LETO and OLETF in aortic endothelial cell-enriched samples. Overall, we found 1) the existence of heterogeneity in the transcriptional profile of the SFA and GFA within healthy LETO rats, 2) that this between-vessel heterogeneity was markedly exacerbated in the hyperphagic, obese OLETF rat, and 3) a greater number of genes whose expression was altered by obesity in the SFA compared with the GFA. Also, results indicate that in OLETF rats the GFA takes on a relatively more proatherogenic phenotype compared with the SFA.

scholarly journals Transcriptome-wide RNA sequencing analysis of rat skeletal muscle feed arteries. II. Impact of exercise training in obesity

2014 ◽  
Vol 116 (8) ◽  
pp. 1033-1047 ◽  
Author(s):  
Jaume Padilla ◽  
Nathan T. Jenkins ◽  
Pamela K. Thorne ◽  
Jeffrey S. Martin ◽  
R. Scott Rector ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Endothelial Cells ◽  
Exercise Training ◽  
Training Program ◽  
Rna Sequencing ◽  
Gastrocnemius Muscle ◽  
Aortic Endothelial Cells ◽  
Feed Arteries ◽  
Skeletal Muscle Feed Arteries ◽  
Aortic Endothelial

We employed next-generation RNA sequencing (RNA-Seq) technology to determine the extent to which exercise training alters global gene expression in skeletal muscle feed arteries and aortic endothelial cells of obese Otsuka Long-Evans Tokushima Fatty (OLETF) rats. Transcriptional profiles of the soleus and gastrocnemius muscle feed arteries (SFA and GFA, respectively) and aortic endothelial cell-enriched samples from rats that underwent an endurance exercise training program (EndEx; n = 12) or a interval sprint training program (IST; n = 12) or remained sedentary (Sed; n = 12) were examined. In response to EndEx, there were 39 upregulated (e.g., MANF) and 20 downregulated (e.g., ALOX15) genes in SFA and 1 upregulated (i.e., Wisp2) and 1 downregulated (i.e., Crem) gene in GFA [false discovery rate (FDR) < 10%]. In response to IST, there were 305 upregulated (e.g., MANF, HSPA12B) and 324 downregulated genes in SFA and 101 upregulated and 66 downregulated genes in GFA, with an overlap of 32 genes between arteries. Furthermore, in aortic endothelial cells, there were 183 upregulated (e.g., eNOS, SOD-3) and 141 downregulated (e.g., ATF3, Clec1b, npy, leptin) genes with EndEx and 71 upregulated and 69 downregulated genes with IST, with an overlap of 35 between exercise programs. Expression of only two genes (Tubb2b and Slc9a3r2) was altered (i.e., increased) by exercise in all three arteries. The finding that both EndEx and IST produced greater transcriptional changes in the SFA compared with the GFA is intriguing when considering the fact that treadmill bouts of exercise are associated with greater relative increases in blood flow to the gastrocnemius muscle compared with the soleus muscle.

scholarly journals Transcriptome‐wide RNA sequencing analysis of rat skeletal muscle feed arteries (1156.12)

2014 ◽  
Vol 28 (S1) ◽  
Author(s):  
Nathan Jenkins ◽  
Jaume Padilla ◽  
Pamela Thorne ◽  
Jeffrey Martin ◽  
R Rector ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Rna Sequencing ◽  
Sequencing Analysis ◽  
Rat Skeletal Muscle ◽  
Feed Arteries ◽  
Skeletal Muscle Feed Arteries

Human aortic endothelial cell aminopeptidase N

1996 ◽  
Vol 32 (1-3) ◽  
pp. 153-156 ◽  
Author(s):  
Andreas Papapetropoulos ◽  
James W Ryan ◽  
Alexander Antonov ◽  
Renu Virmani ◽  
Frank D Kolodgie ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Aminopeptidase N ◽  
Human Aortic Endothelial Cell ◽  
Aortic Endothelial Cell ◽  
Aortic Endothelial

scholarly journals Porcine Aortic Endothelial Cell Genes Responsive to Selected Inflammatory Stimulators

2009 ◽  
Vol 71 (11) ◽  
pp. 1499-1508 ◽  
Author(s):  
Hye-Jung YEOM ◽  
Kum-Joo SHIN ◽  
Jun-Sub KIM ◽  
Seung-Jun KIM ◽  
Sukmook LEE ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Porcine Aortic Endothelial Cell ◽  
Aortic Endothelial Cell ◽  
Aortic Endothelial

Aging induces muscle-specific impairment of endothelium-dependent dilation in skeletal muscle feed arteries

2002 ◽  
Vol 93 (5) ◽  
pp. 1685-1690 ◽  
Author(s):  
Christopher R. Woodman ◽  
Elmer M. Price ◽  
M. Harold Laughlin
Keyword(s):  
Skeletal Muscle ◽  
Mrna Expression ◽  
Vascular Function ◽  
Fischer 344 ◽  
Old Rats ◽  
The Right ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide ◽  
Feed Arteries ◽  
Skeletal Muscle Feed Arteries

We tested the hypothesis that aging decreases endothelium-dependent vasodilation in feed arteries perfusing rat skeletal muscle. In addition, we tested the hypothesis that attenuated vasodilator responses are associated with decreased endothelial nitric oxide synthase (eNOS) and superoxide dismutase-1 (SOD-1) expression. Soleus feed arteries (SFA) and gastrocnemius feed arteries (GFA) were isolated from young (4 mo) and old (24 mo) male Fischer 344 rats. Feed arteries from the right hindlimb were cannulated with two glass micropipettes for examination of endothelium-dependent [acetylcholine (ACh)] and endothelium-independent [adenosine (Ado) or sodium nitroprusside (SNP)] vasodilator function. Feed arteries from the left hindlimb were frozen and used to assess eNOS and SOD-1 protein and mRNA expression. In SFA, endothelium-dependent dilation to ACh was reduced in old rats (0.9 ± 0.04 vs. 0.8 ± 0.03), whereas dilator responses to Ado and SNP were similar in SFA of young and old rats. In GFA, vasodilator responses to ACh, Ado, and SNP were not altered by age. eNOS and SOD-1 protein expression declined with age in SFA (−71 and −54%, respectively) but not in GFA. eNOS and SOD-1 mRNA expression were not altered by age in SFA or GFA. Collectively, these data indicate aging induces muscle-specific impairment of endothelium-dependent vascular function in SFA.

scholarly journals Effects of macro- versus nanoporous silicon substrates on human aortic endothelial cell behavior

2014 ◽  
Vol 9 (1) ◽  
pp. 421 ◽  
Author(s):  
Pilar Formentín ◽  
María Alba ◽  
Úrsula Catalán ◽  
Sara Fernández-Castillejo ◽  
Josep Pallarès ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Behavior ◽  
Silicon Substrates ◽  
Human Aortic Endothelial Cell ◽  
Nanoporous Silicon ◽  
Aortic Endothelial Cell ◽  
Aortic Endothelial
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