scholarly journals Short Communication: Subtyping ofMycobacterium kansasiiby PCR-Restriction Enzyme Analysis of thehsp65Gene

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
Zofia Bakuła ◽  
Aleksandra Safianowska ◽  
Magdalena Nowacka-Mazurek ◽  
Jacek Bielecki ◽  
Tomasz Jagielski
Keyword(s):  
Lung Disease ◽  
Pulmonary Disease ◽  
Restriction Enzyme ◽  
Short Communication ◽  
Nontuberculous Mycobacteria ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Mycobacterium Kansasii ◽  
Clinical Specimens ◽  
Common Causes

Mycobacterium kansasiiis one of the most common causes of pulmonary disease resulting from nontuberculous mycobacteria (NTM). It is also the most frequently isolated NTM species from clinical specimens in Poland. The aim of this study was to investigate the distribution ofM. kansasiisubtypes among patients suspected of having pulmonary NTM disease. Fifty clinical isolates ofM. kansasiirecovered from as many patients with suspected mycobacterial lung disease between 2000 and 2010 in Poland were genotyped by PCR-restriction enzyme analysis (PCR-REA) of partialhsp65gene.Mycobacterium kansasiisubtype I was the only genotype to be identified among the isolates, both disease-associated and non-disease-associated. Isolation ofM. kansasiisubtype I from clinical specimens may be indicative of infection but may also merely represent colonization.

Proposal of a new method for subtyping of Mycobacterium kansasii based upon PCR restriction enzyme analysis of the tuf gene

2016 ◽  
Vol 84 (4) ◽  
pp. 318-321 ◽  
Author(s):  
Zofia Bakuła ◽  
Magdalena Modrzejewska ◽  
Aleksandra Safianowska ◽  
Jakko van Ingen ◽  
Małgorzata Proboszcz ◽  
...  
Keyword(s):  
Restriction Enzyme ◽  
New Method ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Mycobacterium Kansasii ◽  
Tuf Gene

Identification of mycobacterial species by PCR restriction enzyme analysis of thehsp65gene — an Indian experience

2015 ◽  
Vol 61 (4) ◽  
pp. 293-296 ◽  
Author(s):  
Ajoy Kumar Verma ◽  
Gavish Kumar ◽  
Jyoti Arora ◽  
Paras Singh ◽  
Vijay Kumar Arora ◽  
...  
Keyword(s):  
Restriction Enzyme ◽  
Nontuberculous Mycobacteria ◽  
Diagnostic Methods ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Smear Microscopy ◽  
Biochemical Tests ◽  
Mycobacterium Chelonae ◽  
Biochemical Identification ◽  
Pcr Targeting

Nowadays, nontuberculous mycobacteria (NTM) often cause pulmonary and extrapulmonary disease. Species identification of NTM determines the line of treatment and management of the disease. The routine diagnostic methods, i.e., smear microscopy and biochemical identification, of nontuberculous mycobacteria are tedious and time consuming and not all laboratories can perform these tests on a routine basis. A PCR targeting the hsp65 gene was implemented using standard strains and was applied to 109 clinical isolates. The PCR-amplified product was subjected to restriction enzyme analysis using BstEII and HaeIII. The results obtained were compared with that of biochemical tests. Of 109 NTM, 107 were identified to species level. PCR plus restriction enzyme analysis (PRA) identified 12 types of NTM. Common species identified were Mycobacterium chelonae (32), a rapid growing NTM, and Mycobacterium avium complex (21), among the slow growing NTM. PRA and biochemical identification showed 95.32% (102/107) concordant results. PRA is fast, cheap, and accurate for identification of potentially pathogenic NTM.

Long interspersed repeated DNA (LINE) causes polymorphism at the rat insulin 1 locus

1985 ◽  
Vol 5 (9) ◽  
pp. 2197-2203
Author(s):  
M S Lakshmikumaran ◽  
E D'Ambrosio ◽  
L A Laimins ◽  
D T Lin ◽  
A V Furano
Keyword(s):  
Dna Sequence ◽  
Restriction Enzyme ◽  
Allelic Variation ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Repeated Dna ◽  
Coding Region ◽  
Sequence Determination ◽  
Polymorphic Region ◽  
Or Gene

The insulin 1, but not the insulin 2, locus is polymorphic (i.e., exhibits allelic variation) in rats. Restriction enzyme analysis and hybridization studies showed that the polymorphic region is 2.2 kilobases upstream of the insulin 1 coding region and is due to the presence or absence of an approximately 2.7-kilobase repeated DNA element. DNA sequence determination showed that this DNA element is a member of a long interspersed repeated DNA family (LINE) that is highly repeated (greater than 50,000 copies) and highly transcribed in the rat. Although the presence or absence of LINE sequences at the insulin 1 locus occurs in both the homozygous and heterozygous states, LINE-containing insulin 1 alleles are more prevalent in the rat population than are alleles without LINEs. Restriction enzyme analysis of the LINE-containing alleles indicated that at least two versions of the LINE sequence may be present at the insulin 1 locus in different rats. Either repeated transposition of LINE sequences or gene conversion between the resident insulin 1 LINE and other sequences in the genome are possible explanations for this.

Restriction Enzyme Analysis (REA) of Group A Streptococcal (GAS) M-Serotypes 1, 3, and 28

1997 ◽  
pp. 221-223 ◽  
Author(s):  
Dwight R. Johnson ◽  
Cheryl L. Romana ◽  
Carey D. Rehder ◽  
Joanne Dehnbostel ◽  
Edward L. Kaplan
Keyword(s):  
Restriction Enzyme ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Group A
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