scholarly journals Cloud-Point Extraction Combined with Liquid Chromatography for the Determination of Ergosterol, a Natural Product with Diuretic Activity, in Rat Plasma, Urine, and Faeces

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Dan-Qian Chen ◽  
Jun-Min An ◽  
Ya-Long Feng ◽  
Ting Tian ◽  
Xiang-Yang Qin ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Cloud Point ◽  
Cloud Point Extraction ◽  
Rat Plasma ◽  
Ionic Surfactant ◽  
Pharmacokinetic Studies ◽  
Triton X

Ergosterol from many medicinal fungi has been demonstrated to possess a variety of pharmacological activitiesin vivoandin vitro. A new method based on cloud-point extraction has been developed, optimized and validated for the determination of ergosterol in rat plasma, urine and faeces by liquid chromatography. The non-ionic surfactant Triton X-114 was chosen as the extract solvent. The chromatographic separation was performed on an Inertsil ODS-3 analytical column with a mobile phase consisting of methanol and water (98 : 2, v/v) at a flow rate of 1 mL/min. The methodology was validated completely. The results indicated good performance in terms of specificity, linearity, detection and quantification limits, precision and accuracy. The method was successfully applied to the pharmacokinetic studies of ergosterol in rats. The results indicate that the ergosterol levels in feces are much higher than those in plasma and urine of the rat.

scholarly journals Cloud Point Extraction for Pre-Concentration and Spectrophotometric Determination of Trace Amounts of Silver Ions

2020 ◽  
Vol 10 (03) ◽  
pp. 459-464
Author(s):  
Azhar A. Ghali
Keyword(s):  
Detection Limit ◽  
Cloud Point ◽  
Spectrophotometric Determination ◽  
Cloud Point Extraction ◽  
Incubation Temperature ◽  
Chelating Agent ◽  
Silver Ions ◽  
Ionic Surfactant ◽  
Triton X

During the last years, the cloud point extraction was applied for determination, separation, and enrichment of the elements. The current study used cloud point extraction (CPE) for the extraction of Ag(I) ions pre-concentration from watery solutions by a non-ionic surfactant (Triton X-114) and chelating agent a 6-(4-bromo-phenylazo)m-anisidine[6-(4-BrPAA)], then estimation by using the spectrophotometry at 514 nm. Several condition effects on the efficiency of the cloud-point extraction included Triton X-114 concentration, [6-(4-BrPAA)] concentration, pH, time, and incubation temperature. The silver reacts with [6-(4-BrPAA)] to produce complex at a ratio of one to one. 0.009 to 1.5 μg mL-1 is the range of linearity. The detection limit and quantification of Ag(I) ion were 0.0054 and 0.0182 μg mL-1, respectively. The interference of the cations was examined. The cloud-point extraction was used for the evaluation of silver concentration in the water specimen.

Selective Ligandless Cloud Point Extraction of Palladium from Water and Dust Samples

2015 ◽  
Vol 98 (1) ◽  
pp. 201-205 ◽  
Author(s):  
Sayed Zia Mohammadi ◽  
Mohsen Mohammadnezhad
Keyword(s):  
Cloud Point ◽  
High Efficiency ◽  
Cloud Point Extraction ◽  
Absorption Spectrometry ◽  
Ionic Surfactant ◽  
Solution Ph ◽  
Flame Atomic Absorption ◽  
Separation And Preconcentration ◽  
Triton X

Abstract In this study, the phase-separation phenomenon ofnon-ionic surfactants was used for separation and preconcentration of Pd(II). The cloud point extraction(CPE) method is based on the formation of PdI2 which is then entrapped in the non-ionic surfactant Triton X-114. Ethanol acidified with 0.5 M HNO3 was added to the surfactant-rich phase prior to its analysis by flame atomic absorption spectrometry. The main factors affecting CPE efficiency, such as sample solution pH, concentration of iodide ion and Triton X-114, equilibration temperature and time, were all investigated and optimized. At optimum conditions, a calibration curve was constructed for the determination of palladium according to the ligandless CPE procedure. Linearity was maintained between 1.0 to 500.0 ng/mL. The LOD based on three times the SD of the blank divided by the slope of analytical curve, (3Sb/m) was 0.3 ng/mL. Seven replicatedeterminations of a solution containing of 4.0 μg palladium gave a mean absorbance of 0.359 with RSD ±1.85%. The high efficiency of CPE to carry out the determination of palladium in complex matrixes was demonstrated. The proposed method has beenapplied to the determination of trace amounts of palladium in a platinum-iridium alloy, water, and dust samples, with satisfactory results.

Simultaneous determination of vasicine and its major metabolites in rat plasma by UPLC-MS/MS and its application to in vivo pharmacokinetic studies

RSC Advances ◽  
2015 ◽  
Vol 5 (95) ◽  
pp. 78336-78351 ◽  
Author(s):  
Wei Liu ◽  
Dandan He ◽  
Yudan Zhu ◽  
Xuemei Cheng ◽  
Hao Xu ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Pharmacokinetic Study ◽  
Rat Plasma ◽  
Pharmacokinetic Studies ◽  
Butyrylcholinesterase Activity

An UPLC-MS/MS method was developed to simultaneously determinate vasicine and its main metabolites and applied to the pharmacokinetic study. In addition, the anti-butyrylcholinesterase activity of component in plasma was evaluatedin vitro.

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