scholarly journals An In Vitro Analysis of the Effects of Intravenous Lipid Emulsion on Free and Total Local Anaesthetic Concentrations in Human Blood and Plasma

2014 ◽  
Vol 2014 ◽  
pp. 1-7
Author(s):  
Louise Ann Clark ◽  
Jochen Beyer ◽  
Andis Graudins
Keyword(s):  
Blood Plasma ◽  
Human Blood ◽  
Local Anaesthetic ◽  
Whole Blood ◽  
Lipid Emulsion ◽  
Equilibrium Dialysis ◽  
Human Blood Plasma ◽  
Intravenous Lipid Emulsion ◽  
Vitro Effect

Background. Intravenous lipid emulsion (ILE) is recommended as a “rescue” treatment for local anaesthetic (LA) toxicity. A purported mechanism of action suggests that lipophilic LAs are sequestered into an intravascular “lipid-sink,” thus reducing free drug concentration. There is limited data available correlating the effects of ILE on LAs.Aims. To compare the in vitro effect of ILE on LA concentrations in human blood/plasma and to correlate this reduction to LA lipophilicity.Method. One of four LAs (bupivacaine-most lipophilic-4 mg/L, ropivacaine-6 mg/L, lignocaine-14 mg/L, and prilocaine-least lipophilic-7 mg/L) was spiked into plasma or whole blood. ILE or control-buffer was added. Plasma was centrifuged to separate ILE and total-LA concentration assayed from the lipid-free fraction. Whole blood underwent equilibrium dialysis and free-LA concentration was measured. Percent reduction in LA concentration from control was compared between the LAs and correlated with lipophilicity.Results. ILE caused a significant reduction in total and free bupivacaine concentration compared with the other LAs. Ropivacaine had the least reduction in concentration, despite a lipophilicity similar to bupivacaine. The reduction in LA concentration correlated to increasing lipophilicity when ropivacaine was excluded from analysis.Conclusion. In this first in vitro model assessing both free- and total-LA concentrations exposed to ILE in human blood/plasma, ILE effect was linearly correlated with increasing lipophilicity for all but ropivacaine.

Action of heparin and heparin-precipitated fraction of human blood plasma on antibody-producing cells in vitro

1976 ◽  
Vol 81 (1) ◽  
pp. 70-72
Author(s):  
S. V. Kaznacheev ◽  
V. A. Kozlov ◽  
E. M. Petrova ◽  
V. P. Lozovoi
Keyword(s):  
Blood Plasma ◽  
Human Blood ◽  
Human Blood Plasma

A Novel Validation of Analysis Method for Determining Nicotine Levels in Human Blood Plasma (In vitro) High-Pressure Liquid Chromatography Ultraviolet Detector

2020 ◽  
Vol 10 (02) ◽  
pp. 238-243
Author(s):  
Ghassaq T. Al-Ubaidi ◽  
Ahmed A. Abbas ◽  
Ali A. Taha ◽  
Qasim S. Sharhan
Keyword(s):  
Liquid Chromatography ◽  
Blood Plasma ◽  
Human Blood ◽  
Analytical Methods ◽  
Limit Of Detection ◽  
Uv Detection ◽  
Human Blood Plasma ◽  
Analysis Method ◽  
System Suitability

The necessity of nicotine analysis in blood plasma is increasing along with the increased number of smokers and nicotine poisoning cases. One of the analytical methods for nicotine is using high-performance liquid chromatography (HPLC) with ultraviolet (UV) detector because it has been commonly owned by instance in Indonesia. To guarantee accuracy, an analytical method can be used, and it must be validated. This research was the purpose of finding out the validity of the nicotine analysis method in human blood plasma (in vitro) using HPLC with UV detection. Blood plasma samples remained treated with centrifugation procedure by protein denaturation method using acetonitrile. The compounds were analyzed using methanol and buffer acetate 0.01 M (pH 5) 85:15 v/v as a mobile phase on an octadecylsilane column 250 mm, with UV detection at 254 and 260 nm, and flow rate 0.6 mL/minute. Parameter of analytical methods that were validated includes selectivity, accuracy, precision, repeatability, linearity, limit of detection (LoD), limit of quantification (LoQ), and system suitability. According to the result, the selectivity was 2.479, repeatability expressed by its variation coefficient = 0.701%, linearity at range 5–22 μg/mL expressed by coefficient correlation (r) = 0.996. Based on the chromatogram’s area under a curve, the LoD value was found 2.021 μg/mL, LoQ value was 6.737 μg/mL, the accurate percentage was 112.49 to 114.12%, and precision (% CV) was 2.15 to 3.95%. The system suitability from retention time and chromatogram’s area under curve showed % CV 0.70 and 1.64%. According to the experiment result, all parameters meet the requirements of validation criteria.

Effect of x-ray contrast agents on human blood plasma interleukin-2 level in vitro

1992 ◽  
Vol 114 (3) ◽  
pp. 1315-1317
Author(s):  
Yu. K. Napolov ◽  
N. U. Borsukova ◽  
N. L. Shimanovskii
Keyword(s):  
Blood Plasma ◽  
Contrast Agents ◽  
Human Blood ◽  
Interleukin 2 ◽  
Human Blood Plasma ◽  
X Ray

scholarly journals Behaviour of Titanium Dioxide Particles in Artificial Body Fluids and Human Blood Plasma

2021 ◽  
Vol 22 (19) ◽  
pp. 10614
Author(s):  
Eva Korábková ◽  
Věra Kašpárková ◽  
Daniela Jasenská ◽  
Dita Moricová ◽  
Eliška Daďová ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Human Blood ◽  
Body Fluids ◽  
Human Blood Plasma ◽  
Tio2 Particles ◽  
Commercial Mixture ◽  
Increased Risk ◽  
The Impact

The growing application of materials containing TiO2 particles has led to an increased risk of human exposure, while a gap in knowledge about the possible adverse effects of TiO2 still exists. In this work, TiO2 particles of rutile, anatase, and their commercial mixture were exposed to various environments, including simulated gastric fluids and human blood plasma (both representing in vivo conditions), and media used in in vitro experiments. Simulated body fluids of different compositions, ionic strengths, and pH were used, and the impact of the absence or presence of chosen enzymes was investigated. The physicochemical properties and agglomeration of TiO2 in these media were determined. The time dependent agglomeration of TiO2 related to the type of TiO2, and mainly to the type and composition of the environment that was observed. The presence of enzymes either prevented or promoted TiO2 agglomeration. TiO2 was also observed to exhibit concentration-dependent cytotoxicity. This knowledge about TiO2 behavior in all the abovementioned environments is critical when TiO2 safety is considered, especially with respect to the significant impact of the presence of proteins and size-related cytotoxicity.

scholarly journals In vitro study of dual layer mixed matrix hollow fiber membranes for outside-in filtration of human blood plasma

2021 ◽  
Author(s):  
O.E.M. ter Beek ◽  
M.K. van Gelder ◽  
C. Lokhorst ◽  
D.H.M. Hazenbrink ◽  
B.H. Lentferink ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Human Blood ◽  
Hollow Fiber ◽  
In Vitro Study ◽  
Vitro Study ◽  
Human Blood Plasma ◽  
Hollow Fiber Membranes ◽  
Mixed Matrix ◽  
Fiber Membranes

Egg beater as centrifuge: isolating human blood plasma from whole blood in resource-poor settings

Lab on a Chip ◽  
2008 ◽  
Vol 8 (12) ◽  
pp. 2032 ◽  
Author(s):  
Amy P. Wong ◽  
Malancha Gupta ◽  
Sergey S. Shevkoplyas ◽  
George M. Whitesides
Keyword(s):  
Blood Plasma ◽  
Human Blood ◽  
Whole Blood ◽  
Human Blood Plasma ◽  
Resource Poor
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