scholarly journals Strategies Affording Prevascularized Cell-Based Constructs for Myocardial Tissue Engineering

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Claudio Muscari ◽  
Emanuele Giordano ◽  
Francesca Bonafè ◽  
Marco Govoni ◽  
Carlo Guarnieri
Keyword(s):  
Tissue Engineering ◽  
Cardiac Tissue ◽  
Future Research ◽  
Myocardial Tissue ◽  
Cell Sheets ◽  
Mixed Cell ◽  
Differentiation Potential ◽  
Cardiac Wall ◽  
Basic Approaches ◽  
Myocardial Tissue Engineering

The production of a functional cardiac tissue to be transplanted in the injured area of the infarcted myocardium represents a challenge for regenerative medicine. Most cell-based grafts are unviable because of inadequate perfusion; therefore, prevascularization might be a suitable approach for myocardial tissue engineering. To this aim, cells with a differentiation potential towards vascular and cardiac muscle phenotypes have been cocultured in 2D or 3D appropriate scaffolds. In addition to these basic approaches, more sophisticated strategies have been followed employing mixed-cell sheets, microvascular modules, and inosculation from vascular explants. Technologies exerting spatial control of vascular cells, such as topographical surface roughening and ordered patterning, represent other ways to drive scaffold vascularization. Finally, microfluidic devices and bioreactors exerting mechanical stress have also been employed for high-throughput scaling-up production in order to accelerate muscle differentiation and speeding the endothelialization process. Future research should address issues such as how to optimize cells, biomaterials, and biochemical components to improve the vascular integration of the construct within the cardiac wall, satisfying the metabolic and functional needs of the myocardial tissue.

scholarly journals Production and characterization of elastomeric cardiac tissue-like patches for Myocardial Tissue Engineering

2020 ◽  
Vol 90 ◽  
pp. 106613
Author(s):  
Sumeyye Cesur ◽  
Songul Ulag ◽  
Lara Ozak ◽  
Aleyna Gumussoy ◽  
Sema Arslan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cardiac Tissue ◽  
Myocardial Tissue ◽  
Myocardial Tissue Engineering

NEW BIOARTIFICIAL SYSTEMS AND BIODEGRADABLE SYNTHETIC POLYMERS FOR CARDIAC TISSUE ENGINEERING: A PRELIMINARY SCREENING

2010 ◽  
Vol 22 (06) ◽  
pp. 497-507 ◽  
Author(s):  
Elisabetta Rosellini ◽  
Caterina Cristallini ◽  
Niccoletta Barbani ◽  
Giovanni Vozzi ◽  
Mario D'Acunto ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cardiac Tissue ◽  
Degradation Products ◽  
Synthetic Polymers ◽  
Myocardial Tissue ◽  
Specific Cell ◽  
Biological Characterization ◽  
Proliferation And Differentiation ◽  
Poly Ethylene ◽  
Myocardial Tissue Engineering

The aim of this work was the preparation and characterization of new polymeric biomaterials for application in myocardial tissue engineering. The attention was firstly focused on new bioartificial polymeric systems, with the aim to combine the features of synthetic polymers with the specific cell and tissue compatibility of biopolymers. In this work, alginate, collagen, and gelatin were used as the natural component and poly(N-isopropylacrylamide) was used as the synthetic component. The characterization included morphological, topographical, and mechanical analyses, thermogravimetric characterization, infrared spectroscopy, and cell culture tests. For the biological characterization, C2C12 myoblasts were cultured on different materials and cell adhesion, proliferation, and differentiation were evaluated. The morphological, topographical, and mechanical analyses, as well as the biological characterization, were also applied to a tri-block poly(ester-ether-ester) copolymer, obtained by reaction of preformed poly(ethylene glycol) with ε-caprolactone, and a novel poly(ester urethane) obtained by using an L-lisine-derived diisocyanate, giving nontoxic degradation products. The encouraging results obtained in this work allowed us to select some of the new bioartificial polymers, the synthetic tri-block copolymer, and the polyurethane as potential good materials to prepare scaffolds for myocardial tissue engineering.

Application potential of three-dimensional silk fibroin scaffold using mesenchymal stem cells for cardiac regeneration

2021 ◽  
pp. 088532822110185
Author(s):  
Yuksel Cetin ◽  
Merve G Sahin ◽  
Fatma N Kok
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Silk Fibroin ◽  
Cardiac Tissue ◽  
Troponin I ◽  
Cardiac Tissue Engineering ◽  
Swelling Ratio ◽  
Differentiation Potential ◽  
Cardiomyogenic Differentiation

Cardiac tissue engineering focusing on biomaterial scaffolds incorporating cells from different sources has been explored to regenerate or repair damaged area as a lifesaving approach.The aim of this study was to evaluate the cardiomyocyte differentiation potential of human adipose mesenchymal stem cells (hAD-MSCs) as an alternative cell source on silk fibroin (SF) scaffolds for cardiac tissue engineering. The change in surface morphology of SF scaffolds depending on SF concentration (1–6%, w/v) and increase in their porosity upon application of unidirectional freezing were visualized by scanning electron microscopy (SEM). Swelling ratio was found to increase 2.4 fold when SF amount was decreased from 4% to 2%. To avoid excessive swelling, 4% SF scaffold with swelling ratio of 10% (w/w) was chosen for further studies.Biodegradation rate of SF scaffolds depended on enzymatic activity was found to be 75% weight loss of SF scaffolds at the day 14. The phenotype of hAD-MSCs and their multi-linage potential into chondrocytes, osteocytes, and adipocytes were shown by flow cytometry and immunohistochemical staining, respectively.The viability of hAD-MSCs on 3D SF scaffolds was determined as 90%, 118%, and 138% after 1, 7, and 14 days, respectively. The use of 3D SF scaffolds was associated with increased production of cardiomyogenic biomarkers: α-actinin, troponin I, connexin 43, and myosin heavy chain. The fabricated 3D SF scaffolds were proved to sustain hAD-MSCs proliferation and cardiomyogenic differentiation therefore, hAD-MSCs on 3D SF scaffolds may useful tool to regenerate or repair damaged area using cardiac tissue engineering techniques.

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