scholarly journals Using the Doubly Charged Selected Ion Coupled with MS/MS Fragments Monitoring (DCSI-MS/MS) Mode for the Identification of Gelatin Species

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Xian-Long Cheng ◽  
Feng Wei ◽  
Jia Chen ◽  
Ming-hua Li ◽  
Lei Zhang ◽  
...  
Keyword(s):  
Ion Trap ◽  
Time Interval ◽  
Complex Samples ◽  
Ion Trap Mass Spectrometer ◽  
Multiply Charged ◽  
Species Specific ◽  
Doubly Charged ◽  
Charged Ions ◽  
Hydrolysis Of

In electrospray ionization (ESI) mode, peptides and proteins can be multiply charged ions; in this situation a doubly charged selected ion (DCSI) coupled with mass spectrometry (MS/MS) fragments monitoring (DCSI-MS/MS) method is the most suitable scanning mode to detect known peptides in complex samples when an ion-trap mass spectrometer is the instrument used for the analysis. In this mode, the MS detector is programmed to only select a doubly charged ion as a precursor and to perform continuous MS/MS on one or more of the selected precursors, either during a specific time interval or along the whole chromatographic run. Gelatin is a mixture of high molecular weight polypeptides from the hydrolysis of collagen. In this study, the DCSI-MS/MS monitoring mode was applied to the detection of previously characterized species-specific peptides from different gelatins. The proposed methodology makes use of tryptic digestion for sample preparation and peptide separation and identification by rapid resolution liquid chromatography coupled to an ion trap working in the DCSI-MS/MS mode for the analysis. This methodology was applied to the differential classification of five commercial, homological species of gelatins and proved to be an excellent tool for gelatin product authentication.

scholarly journals Formation and fragmentation of doubly and triply charged ions in the negative ion spectra of neutral N-glycans from viral and other glycoproteins

2021 ◽  
Author(s):  
David J. Harvey ◽  
Weston B. Struwe ◽  
Anna-Janina Behrens ◽  
Snezana Vasiljevic ◽  
Max Crispin
Keyword(s):  
Detailed Comparison ◽  
Negative Ion ◽  
Doubly Charged Ions ◽  
Multiply Charged ◽  
Structural Details ◽  
Different Types ◽  
Doubly Charged ◽  
Ion Collision ◽  
Charged Ions

AbstractStructural determination of N-glycans by mass spectrometry is ideally performed by negative ion collision-induced dissociation because the spectra are dominated by cross-ring fragments leading to ions that reveal structural details not available by many other methods. Most glycans form [M – H]- or [M + adduct]- ions but larger ones (above approx. m/z 2000) typically form doubly charged ions. Differences have been reported between the fragmentation of singly and doubly charged ions but a detailed comparison does not appear to have been reported. In addition to [M + adduct]- ions (this paper uses phosphate as the adduct) other doubly, triply, and quadruply charged ions of composition [Mn + (H2PO4)n]n- have been observed in mixtures of N-glycans released from viral and other glycoproteins. This paper explores the formation and fragmentation of these different types of multiply charged ions with particular reference to the presence of diagnostic fragments in the CID spectra and comments on how these ions can be used to characterize these glycans. Graphical abstract

Evidence from SIMS of Doubly-Charged Boron Contamination During Singly Charged-Ion Implantation

1990 ◽  
Vol 48 (2) ◽  
pp. 316-317
Author(s):  
D. S. Simons ◽  
P. H. Chi ◽  
D. B. Novotny
Keyword(s):  
Ion Implantation ◽  
Main Peak ◽  
Charged State ◽  
Exchange Reactions ◽  
Doubly Charged Ions ◽  
Multiply Charged ◽  
Residual Gas ◽  
Singly Charged ◽  
Doubly Charged ◽  
Charged Ions

When a dopant is introduced into a semiconductor material by ion implantation, it is sometimes desirable to accelerate and implant the ion in a multiply-charged state. This has the effect of increasing the energy and range of the ion without increasing the accelerating potential. Most modern ion implanters are of the pre-analysis type. In this design the ions are first accelerated through a modest extraction potential, e.g., 25 keV. This is followed by deflection for mass-to-charge selection in an analyzer magnet, after which the selected ions undergo final acceleration. Charge-exchange reactions between the doubly-charged ions and residual gas have been found to occur between the analyzing magnet and the final acceleration section. These reactions produce singly-charged ions that receive only half of the energy of the doubly-charged ions during final acceleration. For the case of B++ implantation the resulting implant profile shows a shallow-depth shoulder due to B+, the amplitude of which may be greater than 50% of the main peak.

Massenspektroskopische Festkörperuntersuchungen verbesserter Reproduzierbarkeit mit dem Gleichstrom-Abreißfunken im Vakuum zur lonenerzeugung

1963 ◽  
Vol 18 (8-9) ◽  
pp. 926-941 ◽  
Author(s):  
K. D. Schuy ◽  
H. Hintenberger
Keyword(s):  
Mass Spectra ◽  
Multiply Charged Ions ◽  
Doubly Charged Ions ◽  
Multiply Charged ◽  
Singly Charged ◽  
Main Component ◽  
Doubly Charged ◽  
Charged Ions ◽  
Spectrum Evaluation ◽  
Speed Of Analysis

Mass spectra obtained with the disjunctive d.c.-spark in vacuum show considerable improvement in accuracy and reproducibility over the conventional r.f.-spark of the DEMPSTER type. Higher ion currents increase the speed of analysis. A number of mass spectra were produced with a spectroscopic steel standard. The methods of visual and photometric spectrum evaluation are discussed in detail, using two quantities defined as “element sensitivity” and “normalized ionization sensitivity”. The former is a measure of how much more sensitive a given element can be photographically detected with the mass spectrograph than the main component of the sample (matrix element), while the latter indicates how much more sensitive multiply-charged ions of an element can be detected on the plate than singly-charged ions of the same element. Both element- and ionization sensitivities are reproducible to within approximately 20%. Furthermore, it is found, for most elements investigated, that the lines due to doubly-charged ions are more intense than those due to singly-charged ions and that the differences of element sensitivities of various elements decrease for ions of higher charge. The reproducibility of multiply-charged ions permits their use in the quantitative analysis of the sample.

ELECTRON TRANSFER TO MULTIPLY CHARGED IONS OF Ar, N2, N, AND O2

1967 ◽  
Vol 45 (4) ◽  
pp. 1451-1467 ◽  
Author(s):  
J. William McGowan ◽  
Larkin Kerwin
Keyword(s):  
Electron Transfer ◽  
Cross Sections ◽  
Large Scatter ◽  
Single Charge ◽  
Doubly Charged Ions ◽  
Multiply Charged ◽  
Double Electron ◽  
Doubly Charged ◽  
Charged Ions ◽  
Small Scatter

Cross sections for the transfer of one and of two electrons to fast doubly charged ions of Ar, O, N2, N, and to the triply charged ion of Ar are presented. The 20/02 reaction of Ar++ in Ar is resonant and smaller than that for Ar+ in Ar. The nonresonant, single-charge transfer process 20/11, even though it is exothermic, required that 12.5 ± 2.0 eV be transferred to the reaction from the kinetic energy of the projectile for the reaction to go. Consequently, the scatter of the fast Ar+ products is very large. Similarly, large scatter is observed for the double-electron transfer to Ar+++ as the ion traverses an argon target. Unlike the above, however, single- and double-electron transfer to O++ from O2 and to N2++ from N2, double transfer from Ar to Ar++, and single and triple transfer from Ar to Ar+++ show but small scatter.

Structural characterizations of lipids A by MS/MS of doubly charged ions on a hybrid linear ion trap/orbitrap mass spectrometer

2008 ◽  
Vol 43 (4) ◽  
pp. 478-484 ◽  
Author(s):  
Alba Silipo ◽  
Cristina De Castro ◽  
Rosa Lanzetta ◽  
Antonio Molinaro ◽  
Michelangelo Parrilli ◽  
...  
Keyword(s):  
Mass Spectrometer ◽  
Ion Trap ◽  
Linear Ion Trap ◽  
Doubly Charged Ions ◽  
Orbitrap Mass Spectrometer ◽  
Doubly Charged ◽  
Charged Ions ◽  
Structural Characterizations

Non-adiabatic interactions in charge transfer collisions

Open Physics ◽  
2013 ◽  
Vol 11 (9) ◽  
Author(s):  
Marie-Christine Bacchus-Montabonel ◽  
Emese Rozsályi ◽  
Erika Bene ◽  
Gábor Halász ◽  
Ágnes Vibók
Keyword(s):  
Charge Transfer ◽  
Ab Initio ◽  
Collision Energy ◽  
Molecular Targets ◽  
Collision Dynamics ◽  
Charge Transfer Mechanism ◽  
Multiply Charged ◽  
Doubly Charged ◽  
Molecular Calculations ◽  
Charged Ions

AbstractAn analysis of the charge transfer mechanism in the collision of multiply charged ions with molecular and biomolecular targets is performed, considering the non-adiabatic interactions between the molecular states involved. Collisions of doubly charged C 2+ ions on small molecular targets, CO and OH, have been investigated, together with the analysis of charge transfer between C 4+ ions on uracil and halouracil biomolecular targets. The process is studied theoretically by means of ab-initio molecular calculations followed by a semi-classical treatment of the collision dynamics. The influence of rotational couplings is discussed with regard to the collision energy. Strong anisotropic and vibration effects are pointed out.

REACTIONS OF DOUBLY CHARGED IONS WITH VARIOUS NEUTRALS

1979 ◽  
Vol 40 (C7) ◽  
pp. C7-21-C7-22
Author(s):  
K. Peska ◽  
E. Alge ◽  
H. Villinger ◽  
H. Störi ◽  
W. Lindinger
Keyword(s):  
Doubly Charged Ions ◽  
Doubly Charged ◽  
Charged Ions
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