scholarly journals The Quality Assessment of Stored Red Blood Cells Probed Using Atomic-Force Microscopy

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
I. M. Lamzin ◽  
R. M. Khayrullin
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Morphological Characteristics ◽  
High Increase ◽  
Mean Values ◽  
Force Microscopy ◽  
Atomic Force ◽  
The Mean ◽  
The Moment

At the moment the suitability of stored red blood cells (sRBC) for transfusion is checked by routine methods such as haemoglobin estimation and the level of haemolysis. These methods cannot characterize directly the quality of the membranes of sRBC. The aim of this work is to assess the quality of sRBC based on such criteria as the membrane’s stiffness and the size and the form of sRBC. Materials and Methods. We have investigated 5 series of dry cytosmears of the sRBC which had been kept in blood bank in a period from 1 to 35 days. After AFM imaging, in every specimen, 5 RBC were chosen at random; the diameter, the height, and the stiffness were measured on each of them. Results. The present study shows high increase of the mean values of YM and height of RBC after 35 days of storage and decrease of the mean values of their diameter. Conclusion. Statistically significant high increase of the mean values of YM indicates the decrease of the elasticity of the cells in the course of storing of the RBC. This parameter along with the morphological characteristics can be used as criterion for assessment of applicability of the sRBC for blood transfusion.

Erythroplateletapheresis: A New Strategy in the Global Apheresis Program

1998 ◽  
Vol 21 (6_suppl) ◽  
pp. 17-19
Author(s):  
G. Menichella ◽  
M. Ciarli ◽  
R. Serafini ◽  
L. Pierelli ◽  
M. Vittori ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Plasma Volume ◽  
Blood Cells ◽  
Storage Time ◽  
Blood Donation ◽  
Mean Values ◽  
Packed Red Blood Cells ◽  
New Strategy ◽  
The Mean

Blood donation allowed by cell separators can offer higher performance and higher yield to guarantee better quality and pureness of collected products. New systems for the collection of platelet concentrate (PC) and packed red blood cells (PRBC) are currently available. The aim of our work was to test the possibility of preparing PC routinely from normal apheresis donors in a minimum amount of time while providing a second product. Over a 3-month period we performed 40 procedures using the Hemonetics MCS3P blood cell separator and the Dideco Excel. The mean values of platelet yield were 2.8 x 1011 (range 1.4-4.1) with the MCS3P and 3.49 x 1011 (range 2.9-3.9) with the Excel, in a plasma volume of 240 ml and 215 ml respectively; the PRBC units were added with SAG-Mannitol allowing a storage time of 42 days. Collection times were 71’ and 48’ respectively. Donor tolerance was analogous to phateletapheresis or plasmapheresis.

Investigation of the influence of phenosan, ichphan-10, and melafen on red blood cells in vivo by atomic force microscopy

2011 ◽  
Vol 441 (1) ◽  
pp. 245-247 ◽  
Author(s):  
V. I. Binyukov ◽  
O. M. Alekseeva ◽  
E. M. Mil ◽  
A. A. Albantova ◽  
S. G. Fattachov ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Usage of atomic force microscopy for detection of the damaging effect of CdCl2 on red blood cells membrane

2021 ◽  
Vol 208 ◽  
pp. 111683
Author(s):  
E.L. Demchenkov ◽  
A.A. Nagdalian ◽  
R.O. Budkevich ◽  
N.P. Oboturova ◽  
A.I. Okolelova
Keyword(s):  
Atomic Force Microscopy ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Stage-Dependent Topographical and Optical Properties of Plasmodium Falciparum-Infected Red Blood Cells

2021 ◽  
Author(s):  
Katharina Preißinger ◽  
Beáta Vértessy ◽  
István Kézsmárki ◽  
Miklós Kellermayer ◽  
Petra Molnár
Keyword(s):  
Plasmodium Falciparum ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Imaging Study ◽  
Close Correlation ◽  
Functional Changes ◽  
Force Microscopy ◽  
Atomic Force ◽  
Infected Rbcs ◽  
Bright Field Microscopy

Abstract Efficient malaria treatment is a major healthcare challenge. Addressing this challenge requires in-depth understanding of malaria parasite maturation during the intraerythrocytic cycle. Exploring the structural and functional changes of the parasite through the intraerythrocytic stages and their impact on red blood cells (RBCs) is a cornerstone of antimalarial drug development. In order to precisely trace such changes, we performed a thorough imaging study of RBCs infected by Plasmodium falciparum, by using atomic force microscopy (AFM) and total internal reflection fluorescence microscopy (TIRF) supplemented with bright field microscopy for stage assignment. This multifaceted imaging approach allows to reveal structure–function relations via correlations of the parasite maturation with morphological and fluorescence properties of the stages. We established diagnostic patterns characteristic to the parasite stages based on the topographical profile of infected RBCs, which show close correlation with their fluorescence (TIRF) map. Furthermore, we found that hemozoin crystals exhibit a strong optical contrast, possibly due to the quenching of fluorescence. The topographical and optical features provide a tool for locating the hemozoin crystals within the RBCs and following their growth.

scholarly journals Coronavirus SARS-CoV-2: Hypotheses of Impact on the Circulatory System, Prospects for the Use of Perfluorocarbon Emulsion, and Feasibility of Biophysical Research Methods

2020 ◽  
Vol 16 (3) ◽  
pp. 4-13 ◽  
Author(s):  
V. V. Moroz ◽  
A. M. Chernysh ◽  
Elena K. Kozlova
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Molecular Mechanisms ◽  
Circulatory System ◽  
High Resolution Spectroscopy ◽  
Perfluorocarbon Emulsion ◽  
Erythrocyte Morphology ◽  
Force Microscopy ◽  
Human Red Blood Cells ◽  
Atomic Force

This paper highlights published hypotheses on the possibility of coronavirus SARS-CoV-2 entry into the bloodstream, its interaction with vascular endothelium, red blood cells, hemoglobin and its fragments. As a result of such interaction, iron ions may be released into the bloodstream and, subsequently, a cytokine storm may occur. In this context, it is important to find a cytoprotective agent capable of blocking such processes. The perfluorocarbon emulsion could be a candidate for this role.The aim of the paper is to show the feasibility of biophysical methods to study the molecular mechanisms of action of SARS-CoV-2 on human red blood cells and hemoglobin as well as the restorative and cytoprotective effect of the perfluorocarbon emulsion during Fe2+ oxidation in heme.Materials and methods. High resolution spectroscopy, atomic force microscopy, atomic force spectroscopy, electroporation were used. Blood was exposed to oxidizing agents of different nature. Perfluorocarbon emulsion was added in various concentrations and its effect at various incubation times was studied. Concentration of hemoglobin derivatives was calculated considering multicollinearity, and statistical analysis of the results was performed.Results. The perfluorocarbon emulsion was shown to have an effective restorative and cytoprotective action in iron ion oxidation in the heme: Fe3+ was restored to Fe2+. The degree of MetHb reduction to HbO2 and Hb depended on the concentration of the oxidizing agent and incubation time. We observed a change in MetHb content from 80-90% to 5-12%. The perfluorocarbon emulsion in clinical concentrations helped eliminate local membrane defects and restored normal erythrocyte morphology.Conclusion. In the light of the studied hypotheses, the use of perfluorocarbon emulsion can become an effective method for blocking the consequences of coronavirus effect on the blood cells and restoring a normal gas exchange.

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