scholarly journals Effect of Negative Pressure Therapy on the Inflammatory Response of the Intestinal Microenvironment in a Porcine Septic Model

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Kenneth C. Norbury ◽  
Mary Pat Moyer
Keyword(s):  
Inflammatory Response ◽  
Negative Pressure ◽  
Ischemia Reperfusion ◽  
Group Versus ◽  
Organ Injury ◽  
Swine Model ◽  
M2 Macrophages ◽  
Negative Pressure Therapy ◽  
Pressure Therapy ◽  
Species Response

In a swine model of ischemia/reperfusion injury coupled with sepsis, we have previously shown attenuation of secondary organ injury and decreased mortality with negative pressure therapy (NPT). We hypothesized that NPT modulates the intestinal microenvironment by mediating the innate immune system. Sepsis was induced in 12 anesthetized female pigs. Group 1 (n=6) was decompressed at 12 hrs after injury (T12) and treated with standard of care (SOC), and group 2 (n=6) with NPT for up toT48. Immunoparalysis was evident as lymphocytopenia atT24in both groups; however, survival was improved in the NPT group versus SOC (Odds ratio = 4.0). The SOC group showed significant reduction in lymphocyte numbers compared to NPT group byT48(p<0.05). The capacity of peritoneal fluid to stimulate a robust reactive oxygen species responsein vitrowas greater for the NPT group, peaking atT24for both M1 (p=0.0197) and M2 macrophages (p=0.085). Plasma elicited little if any effect which was confirmed by microarray analysis. In this septic swine model NPT appeared to modulate the intestinal microenvironment, facilitating an early robust, yet transient, host defense mediated by M1 and M2 macrophages. NPT may help overcome immunoparalysis that occurs during inflammatory response to septic injury.

Peritoneal Negative Pressure Therapy Prevents Multiple Organ Injury in a Chronic Porcine Sepsis and Ischemia/Reperfusion Model

Shock ◽  
2010 ◽  
Vol 34 (5) ◽  
pp. 525-534 ◽  
Author(s):  
Brian D. Kubiak ◽  
Scott P. Albert ◽  
Louis A. Gatto ◽  
Kathleen P. Snyder ◽  
Kristopher G. Maier ◽  
...  
Keyword(s):  
Negative Pressure ◽  
Ischemia Reperfusion ◽  
Multiple Organ ◽  
Organ Injury ◽  
Negative Pressure Therapy ◽  
Pressure Therapy ◽  
Multiple Organ Injury

Negative pressure therapy – How many dressing changes are enough?

2012 ◽  
Vol 60 (S 01) ◽  
Author(s):  
S Raab ◽  
T Weimann ◽  
W Sienel ◽  
L Lampl ◽  
M Beyer
Keyword(s):  
Negative Pressure ◽  
Negative Pressure Therapy ◽  
Pressure Therapy

Four Cases of Lower Extremity Ulcers Treated with Negative Pressure Therapy

2015 ◽  
Vol 77 (5) ◽  
pp. 456-460
Author(s):  
Maiko SAKAMOTO-TOYA ◽  
Sho MIAKE ◽  
Satoko SHIBATA-KIKUCHI ◽  
Masutaka FURUE
Keyword(s):  
Lower Extremity ◽  
Negative Pressure ◽  
Negative Pressure Therapy ◽  
Pressure Therapy

Negative Pressure Therapy in Wounds Surgical Treatment

2017 ◽  
Vol 68 (11) ◽  
pp. 2687-2690 ◽  
Author(s):  
Bogdan Mihnea Ciuntu ◽  
Stefan Octavian Georgescu ◽  
Ciprian Cirdeiu ◽  
Daniel Timofte ◽  
Doina Azoicai ◽  
...  
Keyword(s):  
Negative Pressure ◽  
Healing Time ◽  
Negative Pressure Therapy ◽  
Simultaneous Application ◽  
Average Hospital ◽  
Pressure Therapy ◽  
Days Of Therapy ◽  
Functional Consequences ◽  
Time Required ◽  
A Prospective Study

The study aims to assess the significance of negative pressure therapy in the treatment of 1 January 2014 - 31 June 2017. The objectives intend to evaluate the healing time required after applying the method and the functional consequences for the patient. A prospective study was conducted on a sample of 31 patients with various tipe of wounds which were monitored their clinical course between September 2014 - February 2017, following negative pressure therapy. There were used vacuum assisted closure devices (VAC � -Hartman) in order to apply negative pressure to the wound, while complying with specified settings in accordance with patients� outcome. Healing was obtained in all cases, to an average hospital stay of 30 days and 12 days of therapy application.The negative result of microbial cultures was obtained after an average of 7.55 days by simultaneous application of negative pressure and antibiotic treatment according to the antibiogram. After basic treatment of the wound, auxiliary methods such as negative pressure contribute to the healing. Evolution was favorable with wound granulation in 95% cases, which allowed surgery under local anesthesia, and defect was covered with skin graft. VAC therapy falls into the last group of treatments by eliminating healing inhibitors. This regenerates the wound in a damp environment and essentially turns an open wound into a closed system.

Negative Pressure Therapy in the Surgical Treatment of Diabetic Foot

2017 ◽  
Vol 68 (7) ◽  
pp. 1648-1651
Author(s):  
Bogdan Mihnea Ciuntu ◽  
Ciprian Vasiluta ◽  
Robert Negru ◽  
Roxana Hultoana ◽  
Roxana Ciuntu ◽  
...  
Keyword(s):  
Abdominal Wall ◽  
Negative Pressure ◽  
Diabetic Foot ◽  
Bacterial Load ◽  
Abdominal Wall Defects ◽  
Negative Pressure Therapy ◽  
Simultaneous Application ◽  
Average Hospital ◽  
Pressure Therapy ◽  
Days Of Therapy

The study aims to assess the significance of negative pressure therapy in the treatment of diabetic foot.The objectives intend to evaluate the healing time required after applying the method and the functional consequences for the patient. A prospective study was conducted on a sample of 37 patients with diabetic foot were monitored their clinical course between September 2014 - April 2017, following negative pressure therapy. There were used vacuum assisted closure devices (VAC � -Hartman) in order to apply negative pressure to the wound, while complying with specified settings (negative pressure, time of use of a kit) in accordance with patients� outcome.There were monitored changes in wound size (planimetric and volumetric measurement), their bacterial load and duration of treatment. Healing was obtained in all cases, to an average hospital stay of 27.3 days and 8 days of therapy application.The negative result of microbial cultures was obtained after an average of 6.45 days by simultaneous application of negative pressure and antibiotic treatment according to the antibiogram. Skin grafts were necessary to close the defect in 4 cases. After basic treatment of the wound, auxiliary methods such as negative pressure contribute to the healing.In patients with diabetic foot who were required surgical intervention, the use of negative pressure therapy yielded a significant benefit in the preservation of the affected limb, after minimal excision.The results we obtained throughout our experience recommend use of NPTW technique as indication for abdominal wall surgery in closing abdominal wall defects, compartment syndrome and surgical site infection after prosthetic mesh.

scholarly journals Pressure transduction to the thoracic cavity during topical negative pressure therapy of a sternotomy wound

2008 ◽  
Vol 5 (4) ◽  
pp. 579-584 ◽  
Author(s):  
Christian Torbrand ◽  
Richard Ingemansson ◽  
Lotta Gustafsson ◽  
Per Paulsson ◽  
Malin Malmsjö
Keyword(s):  
Negative Pressure ◽  
Thoracic Cavity ◽  
Negative Pressure Therapy ◽  
Pressure Therapy ◽  
Topical Negative Pressure

scholarly journals Focal intra-colon cooling reduces organ injury and systemic inflammation after REBOA management of lethal hemorrhage in rats

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Awadhesh K. Arya ◽  
Kurt Hu ◽  
Lalita Subedi ◽  
Tieluo Li ◽  
Bingren Hu
Keyword(s):  
Inflammatory Response ◽  
Inflammatory Cytokines ◽  
Troponin I ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Balloon Catheter ◽  
Organ Injury ◽  
Upper Body ◽  
Anti Inflammatory ◽  
Pro Inflammatory Cytokines

AbstractResuscitative endovascular balloon occlusion of the aorta (REBOA) is a lifesaving maneuver for the management of lethal torso hemorrhage. However, its prolonged use leads to distal organ ischemia–reperfusion injury (IRI) and systemic inflammatory response syndrome (SIRS). The objective of this study is to investigate the blood-based biomarkers of IRI and SIRS and the efficacy of direct intestinal cooling in the prevention of IRI and SIRS. A rat lethal hemorrhage model was produced by bleeding 50% of the total blood volume. A balloon catheter was inserted into the aorta for the implementation of REBOA. A novel TransRectal Intra-Colon (TRIC) device was placed in the descending colon and activated from 10 min after the bleeding to maintain the intra-colon temperature at 37 °C (TRIC37°C group) or 12 °C (TRIC12°C group) for 270 min. The upper body temperature was maintained at as close to 37 °C as possible in both groups. Blood samples were collected before hemorrhage and after REBOA. The organ injury biomarkers and inflammatory cytokines were evaluated by ELISA method. Blood based organ injury biomarkers (endotoxin, creatinine, AST, FABP1/L-FABP, cardiac troponin I, and FABP2/I-FABP) were all drastically increased in TRIC37°C group after REBOA. TRIC12°C significantly downregulated these increased organ injury biomarkers. Plasma levels of pro-inflammatory cytokines TNF-α, IL-1b, and IL-17F were also drastically increased in TRIC37°C group after REBOA. TRIC12°C significantly downregulated the pro-inflammatory cytokines. In contrast, TRIC12°C significantly upregulated the levels of anti-inflammatory cytokines IL-4 and IL-10 after REBOA. Amazingly, the mortality rate was 100% in TRIC37°C group whereas 0% in TRIC12°C group after REBOA. Directly cooling the intestine offered exceptional protection of the abdominal organs from IRI and SIRS, switched from a harmful pro-inflammatory to a reparative anti-inflammatory response, and mitigated mortality in the rat model of REBOA management of lethal hemorrhage.

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