Matrix Metalloproteinase-9 −1562C/T Gene Polymorphism Is Associated with Diabetic Nephropathy

To investigate the association between the metalloproteinase-9 (MMP9) −1562C/T polymorphism and diabetic nephropathy (DN) in Han Chinese, the patients with type 2 diabetes were collected and divided into the non-DN (NDN) and DN groups; controls were recruited. Genotype and allele frequencies were assessed using polymerase chain reaction and restriction fragment length polymorphism. Results showed that SBP, DBP, HbA1c, UAER, Cr, BUN, TG, and TC were higher in the DN group compared with the control and NDN groups. SBP, HbA1c, and TC in DN patients with the TT and CT genotypes were lower than in those with CC. Compared with controls, the frequency of the T allele in the DN group was significantly lower. The MMP9 −1562C allele, SBP, Cr, BUN, TG, and TC were independent risk factors for DN. All of the above suggested that the MMP9 −1562C/T polymorphism was associated with DN in Han Chinese.

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Identification of verotoxin type 2 variant B subunit genes in Escherichia coli by the polymerase chain reaction and restriction fragment length polymorphism analysis.

Journal of Clinical Microbiology ◽

10.1128/jcm.29.7.1339-1343.1991 ◽

1991 ◽

Vol 29 (7) ◽

pp. 1339-1343 ◽

Cited By ~ 87

Author(s):

S D Tyler ◽

W M Johnson ◽

H Lior ◽

G Wang ◽

K R Rozee

Keyword(s):

Escherichia Coli ◽

Polymerase Chain Reaction ◽

Restriction Fragment Length Polymorphism ◽

Fragment Length Polymorphism ◽

Polymorphism Analysis ◽

Chain Reaction ◽

B Subunit ◽

Polymerase Chain ◽

Restriction Fragment Length

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Association analysis of vitamin D receptor gene polymorphisms in North England population with Type 2 diabetes mellitus

African Health Sciences ◽

10.4314/ahs.v21i1.3 ◽

2021 ◽

Vol 21 (1) ◽

pp. 8-14

Author(s):

Naila Abdul Sattar ◽

Sumera Shaheen ◽

Fatma Hussain ◽

Amer Jamil

Keyword(s):

Vitamin D ◽

Vitamin D Receptor ◽

Fragment Length Polymorphism ◽

Receptor Gene ◽

Vitamin D Receptor Gene ◽

Chain Reaction ◽

The North ◽

Polymerase Chain ◽

Restriction Fragment Length

Background: Numerous diabetes susceptibility loci, include a region consisting vitamin D receptor gene found in chromo- some 12q, have been known using genome wide screens. Aim: The aim of present study is to probe the relationship between polymorphism of vitamin D receptor gene (single nu- cleotide polymorphisms) and type 2 diabetes mellitus (T2DM). Five hundred T2DM patients and 200 healthy subjects with normal HbA1c( ≤ 5.0 %), fasting blood sugar (≤ 120 mg/dL) and random blood sugar (≤ 140 mg/dL) were enrolled. Metholodgy: The genotypes were found by polymerase chain reaction restriction fragment length polymorphism and DNA sequencing. Results: revealed that no considerable differences in frequencies of genotype and allele of the Bsm I and Fok I polymor- phisms between healthy and patients in the North England (For Fok I: OR = 1.11, 95% CI: 0.72–1.12; for Bsm I: OR = 1.35, 95% CI: 0.79–1.98). Conclusion: It is recommended that both following polymorphisms of vitamin D receptor gene may not considerably add to the progression of T2DM in the North England. Keywords: T2DM; DNA Sequencing; Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP); Single nucleotide Polymorphism (SNP);VDR.

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