Training Status as a Marker of the Relationship between Nitric Oxide, Oxidative Stress, and Blood Pressure in Older Adult Women

The purpose of this study was to evaluate the influence of functional fitness and oxidative capacity on the nitric oxide concentration associated with hemodynamic control in older adult women. The sample consisted of 134 women (65.73 ± 6.14 years old). All subjects underwent a physical examination to assess body mass index, waist-hip ratio, body fat measurement by dual energy X-ray absorptiometry, and blood pressure (BP). Training status (TS) was evaluated by indirect determination of maximal oxygen uptake by a treadmill test using Balke protocol modified for older adults. Functional fitness was also evaluated through a “Functional Fitness Battery Test” to determine the general fitness functional index (GFFI). All participants were separated according to the functional fitness (TS1, very weak and weak; TS2, regular; TS3, good and very good). Plasma blood samples were used to evaluate prooxidant and antioxidant activity and nitrite and nitrate concentrations. The general results of this study showed that good levels of TS were related to lower levels of lipoperoxidation and protein damage, higher levels of antioxidant, and higher concentration of nitrite and nitrate. This combination may be responsible for the lower levels of BP in subjects with better TS.

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Effect of Multicomponent Training on Blood Pressure, Nitric Oxide, Redox Status, and Physical Fitness in Older Adult Women: Influence of Endothelial Nitric Oxide Synthase (NOS3) Haplotypes

Oxidative Medicine and Cellular Longevity ◽

10.1155/2017/2578950 ◽

2017 ◽

Vol 2017 ◽

pp. 1-12 ◽

Cited By ~ 5

Author(s):

Atila Alexandre Trapé ◽

Elisangela Aparecida da Silva Lizzi ◽

Thiago Correa Porto Gonçalves ◽

Jhennyfer Aline Lima Rodrigues ◽

Simone Sakagute Tavares ◽

...

Keyword(s):

Nitric Oxide ◽

Blood Pressure ◽

Physical Fitness ◽

Older Adult ◽

Elbow Flexion ◽

Redox Status ◽

Adult Women ◽

Nitrite Concentration ◽

Oxide Synthase ◽

Endothelial Nitric Oxide

The purpose of this study was to verify the influence of the genotype or haplotype (interaction) of the NOS3 polymorphisms [-786T>C, 894G>T (Glu298Asp), and intron 4b/a] on the response to multicomponent training (various capacities and motor skills) on blood pressure (BP), nitrite concentration, redox status, and physical fitness in older adult women. The sample consisted of 52 participants, who underwent body mass index and BP assessments. Physical fitness was evaluated by six-minute walk, elbow flexion, and sit and stand up tests. Plasma/blood samples were used to evaluate redox status, nitrite concentration, and genotyping. Associations were observed between isolated polymorphisms and the response of decreased systolic and diastolic BP and increased nitrite concentration and antioxidant activity. In the haplotype analysis, the group composed of ancestral alleles (H1) was the only one to present improvement in all variables studied (decrease in systolic and diastolic BP, improvement in nitrite concentration, redox status, and physical fitness), while the group composed of variant alleles (H8) only demonstrated improvement in some variables of redox status and physical fitness. These findings suggest that NOS3 polymorphisms and physical training are important interacting variables to consider in evaluating redox status, nitric oxide availability and production, and BP control.

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1412 INDIRECT DETERMINATION OF BLOOD PRESSURE (BP) IN NEONATES

Pediatric Research ◽

10.1203/00006450-198104001-01441 ◽

1981 ◽

Vol 15 ◽

pp. 678-678 ◽

Cited By ~ 1

Author(s):

Sergio O Saia ◽

Frank Mannino ◽

Louis Gluck

Keyword(s):

Blood Pressure ◽

Indirect Determination

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Indirect determination of the blood pressure in the dental pulp

Archives of Oral Biology ◽

10.1016/0003-9969(68)90008-3 ◽

1968 ◽

Vol 13 (8) ◽

pp. 929-IN18 ◽

Cited By ~ 8

Author(s):

D.D. Upthegrove ◽

J.G. Bishop ◽

H.L. Dorman

Keyword(s):

Blood Pressure ◽

Dental Pulp ◽

Indirect Determination

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New Modified Method for Determination of Nitric Oxide Synthase Activity in Plasma of Vitiligo Patients

Oriental Journal Of Chemistry ◽

10.13005/ojc/340536 ◽

2018 ◽

Vol 34 (5) ◽

pp. 2502-2509

Author(s):

Jwan Abdulmohsin Zainulabdeen ◽

Aymen Abdulsattar Al-kinani

Keyword(s):

Oxidative Stress ◽

Nitric Oxide ◽

Nitric Oxide Synthase ◽

Healthy Individuals ◽

Modified Method ◽

Oxide Concentration ◽

Nitric Oxide Concentration ◽

And Gender ◽

Oxide Synthase

Vitiligo is a non-contagious skin disorder that characterized by depigmentation of skin due to melanocyte impairment which may be caused to increase levels of free radicals (such as superoxide and nitric oxide) that causing an increase in oxidative stress. The purpose of this study was measured by the activity of oxide synthase (NOS) by our modified method and nitric oxide concentration in plasma of vitiligo patients. The activity of nitric oxide synthase was determined via a modified method by coupling two methods; the first method was based on converting L-arginine to L-citrulline and nitric oxide and the second was used to measure the concentration of nitric oxide. This modified method was applied to patients with vitiligo disease and healthy individuals who matched in age and gender with patients. The condition of this modified method was optimized and the results revealed the following: the activity of NOS was higher in a solution that contains: Tris buffer (50mM), arginine (100mM), calcium chloride (20mM), and NADPH (5mM) during 30 minutes, meanwhile the precision of this method was 2.03. In the current study, the results show that the levels of NOS activity and nitric oxide were affected by the disease in which both parameters appeared highly significant increases in vitiligo patients (p=0.000 and 0.002 respectively) in comparison with the healthy individuals. Results of the experiments proved that it is possible to depend on the modified method to measure the activity of nitric oxide synthase (NOS). Also, the increased levels of NOS activity and nitric oxide concentration in vitiligo patients support the autocytotoxic hypothesis which suggests that oxidative stress may have a role in melanocyte impairment.

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Office Procedures for the Indirect Determination of Blood Pressure

Clinical and Experimental Optometry ◽

10.1111/j.1444-0938.1978.tb02946.x ◽

1978 ◽

Vol 61 (4) ◽

pp. 137-147

Author(s):

Paul D. Ballard

Keyword(s):

Blood Pressure ◽

Indirect Determination ◽

Office Procedures

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Microphonic Manometer for Indirect Determination of Systolic Blood Pressure in the Rat.

Experimental Biology and Medicine ◽

10.3181/00379727-70-17028 ◽

1949 ◽

Vol 70 (4) ◽

pp. 670-672 ◽

Cited By ~ 168

Author(s):

M. Friedman ◽

S. C. Freed

Keyword(s):

Blood Pressure ◽

Systolic Blood Pressure ◽

Indirect Determination

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Effects of 16 Weeks of Resistance Training on Muscle Quality and Muscle Growth Factors in Older Adult Women with Sarcopenia: A Randomized Controlled Trial

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18136762 ◽

2021 ◽

Vol 18 (13) ◽

pp. 6762

Author(s):

Myong-Won Seo ◽

Sung-Woo Jung ◽

Sung-Woo Kim ◽

Jung-Min Lee ◽

Hyun Chul Jung ◽

...

Keyword(s):

Body Weight ◽

Growth Factors ◽

Resistance Training ◽

Older Adult ◽

Muscle Growth ◽

Muscle Quality ◽

Adult Women ◽

Functional Fitness ◽

Elastic Band ◽

Age Related

This study examined the effects of resistance training on muscle quality, muscle growth factors, and functional fitness in older adult women with sarcopenia. Twenty-two older adult women aged over 65 with sarcopenia were randomly assigned to either resistance training (RT, n = 12) or non-exercise control group (CG, n = 10). The body weight-based and elastic band RT were performed three times a week, 60 min per session, for 16 weeks. Body composition and thigh muscle quality were estimated by dual-energy x-ray absorptiometry (DEXA) and computed tomography (CT), respectively. The muscle growth factors, including growth differentiation factor-8 (GDF-8), growth differentiation factor-15 (GDF-15), activin A, and follistatin, were analyzed via blood samples. Statistical analyses were performed using repeated measures multivariate analysis of variance (MANOVA), analysis of variance (ANOVA), and effect size (i.e., cohen’s d, partial eta square), and the significance level was set at 0.05. The RT group improved their functional fitness, grip strength, gait speed, and isometric muscle strength (p < .01, d > .99; large), while these variables did not change in the CG. An increase in intramuscular fat was only observed in the CG (p < .01, 1.06; large). Muscle growth factors such as follistatin were significantly increased in the RT (p < .05, .81; large), but other variables did not change following resistance training. Sixteen weeks of resistance training improved functional fitness and prevented age-related increases in intramuscular fat in the thigh area. However, there were only some changes in muscle growth factors, such as follistatin, suggesting that the effectiveness of resistance training on muscle growth factors is limited. Body weight-based and elastic band resistance training is an alternative training method for sarcopenia to minimize the age-related adverse effects on muscle function and quality.

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