Expression of Genes Related to Germ Cell Lineage and Pluripotency in Single Cells and Colonies of Human Adult Germ Stem Cells

The aim of this study was to elucidate the molecular status of single human adult germ stem cells (haGSCs) and haGSC colonies, which spontaneously developed from the CD49f MACS and matrix- (collagen−/laminin+ binding-) selected fraction of enriched spermatogonia. Single-cell transcriptional profiling by Fluidigm BioMark system of a long-term cultured haGSCs cluster in comparison to human embryonic stem cells (hESCs) and human fibroblasts (hFibs) revealed that haGSCs showed a characteristic germ- and pluripotency-associated gene expression profile with some similarities to hESCs and with a significant distinction from somatic hFibs. Genome-wide comparisons with microarray analysis confirmed that different haGSC colonies exhibited gene expression heterogeneity with more or less pluripotency. The results of this study confirm that haGSCs are adult stem cells with a specific molecular gene expression profilein vitro, related but not identical to true pluripotent stem cells. Under ES-cell conditions haGSC colonies could be selected and maintained in a partial pluripotent state at the molecular level, which may be related to their cell plasticity and potential to differentiate into cells of all germ layers.

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Effect of 3D scaffold and dynamic culture condition on the global gene expression profile of mouse embryonic stem cells

Biomaterials ◽

10.1016/j.biomaterials.2006.05.053 ◽

2006 ◽

Vol 27 (36) ◽

pp. 5978-5989 ◽

Cited By ~ 109

Author(s):

Hui Liu ◽

Jian Lin ◽

Krishnendu Roy

Keyword(s):

Gene Expression ◽

Stem Cells ◽

Embryonic Stem Cells ◽

Gene Expression Profile ◽

Expression Profile ◽

Culture Condition ◽

Embryonic Stem ◽

Global Gene Expression ◽

Global Gene Expression Profile ◽

3D Scaffold

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Alteration of gene expression profile in mouse embryonic stem cells and neural differentiation deficits by ethephon

Human & Experimental Toxicology ◽

10.1177/0960327120930255 ◽

2020 ◽

Vol 39 (11) ◽

pp. 1518-1527

Author(s):

S Mohammadi Nejad ◽

M Hodjat ◽

SA Mousavi ◽

M Baeeri ◽

MA Rezvanfar ◽

...

Keyword(s):

Gene Expression ◽

Cell Cycle ◽

Stem Cells ◽

Embryonic Stem Cells ◽

Gene Expression Profile ◽

Expression Profile ◽

Neural Differentiation ◽

Embryonic Stem ◽

Mouse Embryonic Stem Cells ◽

Differentiation Potential

Ethephon, a member of the organophosphorus compounds, is one of the most widely used plant growth regulators for artificial ripening. Although million pounds of this chemical is being used annually, the knowledge regarding its molecular toxicity is yet not sufficient. The purpose of this study was to evaluate the potential developmental toxicity of ethephon using embryonic stem cell model. The mouse embryonic stem cells (mESCs) were exposed to various concentrations of ethephon and the viability, cell cycle alteration and changes in the gene expression profile were evaluated using high-throughput RNA sequencing. Further, the effect of ethephon on neural differentiation potential was examined. The results showed that ethephon at noncytotoxic doses induced cell cycle arrest in mESCs. Gene ontology enrichment analysis showed that terms related to cell fate and organismal development, including neuron fate commitment, embryo development and cardiac cell differentiation, were markedly enriched in ethephon-treated cells. Neural induction of mESCs in the presence of ethephon was inhibited and the expression of neural genes was decreased in differentiated cells. Results obtained from this work clearly demonstrate that ethephon affects the gene expression profile of undifferentiated mESCs and prevents neural differentiation. Therefore, more caution against the frequent application of ethephon is advised.

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